Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone

doi:10.7668/hbnxb.2017.02.020

Abstract

Abstract: In order to evaluate the breeding application value of two kind of allelic variations (TaCwi-A1a and TaCwi-A1b)of TaCwi-A1 gene,which was related to wheat kernel weight.In this study,539 wheat varieties (lines)in Yellow and Huai River Valleys Facultative Wheat Zone in China were genotyped using functional markers of TaCwi-A1 to determine the allelic variation types,in which the frequencies of TaCwi-A1a and TaCwi-A1b could be detected.To compare the differences of phenotypic traits of TaCwi-A1a and TaCwi-A1b,thousand kernel weight (TKW)of released varieties,region test varieties,and breeding varieties was evaluated in three environments;and kernel length (KL),kernel width (KW),and kernel area (KA)was also evaluated in one environment.The results showed that the frequencies of TaCwi-A1a and TaCwi-A1b were 65.03% and 34.97% in all test materials,in which the frequency of TaCwi-A1a was significantly higher than TaCwi-A1b.Analysis of phenotypic traits of grain showed that TKW of TaCwi-A1a was significantly higher than TaCwi-A1b in released varieties,region test varieties and breeding varieties.Similarly,KL and KW of TaCwi-A1a were also significantly higher than TaCwi-A1b.This study further verified the additive effect of TaCwi-A1a on phenotypic traits of grain,which showed that TaCwi-A1a was superior allelic variation for the grain weight and its constituent elements.In addition,the study identified the distribution of TaCwi-A1 in Yellow and Huai River Valleys Facultative Wheat Zone in China,and provided useful information for the marker-assisted selection of wheat.

Key words: Common wheat, TaCwi-A1, Molecular marker, Allelic variation, Thousand kernel weight, Kernel length, Kernel width

CLC Number:

S512.01

LIU Yongwei, ZHOU Shuo, WANG Xuezheng, SUN Guozhong, ZHU Jinyong, HAN Qiufen, LI Chunjie, ZHAO He, WANG Haibo. Functional Analysis and Distribution of Allelic Variations of TaCwi-A1 Gene Related to Kernel Weight in Yellow and Huai River Valleys Facultative Wheat Zone[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 131-137. doi: 10.7668/hbnxb.2017.02.020.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.02.020

http://www.hbnxb.net/EN/Y2017/V32/I2/131

References

[1] 林作楫,揭声慧,雷振生,等.近60年黄淮麦区冬小麦育种规律演变研究[J].现代农业科技,2011(24):103-105,108.
[2] 庄巧生.中国小麦品种改良及系谱分析[M].北京:中国农业出版社,2003:497-518.
[3] Wang L,Ge H,Hao C,et al.Identifying loci influencing 1,000-kernel weight in wheat by microsatellite screening for evidence of selection during breeding[J].PLoS One,2012,7(2):e29432.
[4] Ma D Y,Yan J,He Z H,et al.Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers[J].Molecular Breeding,2012,29(1):43-52.
[5] Jiang Q,Hou J,Hao C,et al.The wheat(T. aestivum)sucrose synthase 2 gene(TaSus2)active in endosperm development is associated with yield traits[J].Functional & Integrative Genomics,2011,11(1):49-61.
[6] Su Z,Hao C,Wang L,et al.Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat(Triticum aestivum L.)[J].Theoretical and Applied Genetics,2011,122(1):211-223.
[7] Yang Z,Bai Z,Li X,et al.SNP identification and allelic-specific PCR markers development for TaGW2,a gene linked to wheat kernel weight[J].Theoretical and Applied Genetics,2012,125(5):1057-1068.
[8] Qin L,Hao C,Hou J,et al.Homologous haplotypes,expression,genetic effects and geographic distribution of the wheat yield gene TaGW2[J].BMC Plant Biology,2014,14:107.
[9] Zhang L,Zhao Y,Gao L,et al.TaCKX6-D1,the ortholog of rice OsCKX2,is associated with grain weight in hexaploid wheat[J].The New Phytologist,2012,195(3):574-584.
[10] Chang J,Zhang J,Mao X,et al.Polymorphism of TaSAP1-A1 and its association with agronomic traits in wheat[J].Planta,2013,237(6):1495-1508.
[11] Guo Y,Sun J J,Zhang G Z,et al.Haplotype,molecular marker and phenotype effects associated with mineral nutrient and grain size traits of TaGS1a in wheat[J].Field Crops Research,2013,154(3):119-125.
[12] Zhang Y J,Liu J D,Xia X C,et al.TaGS-D1,an ortholog of rice OsGS3,is associated with grain weight and grain length in common wheat[J].Molecular Breeding,2014,34(3):1097-1107.
[13] Dong L L,Wang F M,Liu T,et al.Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions[J].Molecular Breeding,2014,34(3):937-947.
[14] 韩利明,杨芳萍,夏先春,等.株高、粒重及抗病相关基因在不同国家小麦品种中的分布[J].麦类作物学报,2011,31(5):824-831.
[15] 相吉山,穆培源,桑伟,等.小麦粒重基因TaCwi-A1功能标记CWI22、CWI21的验证及应用[J].中国农业科学,2014,47(13):2671-2709.
[16] 张祖建,郎有忠,潘美红,等.粳稻小粒穗的症状及其籽粒性状的变化特征[J].中国农业科学,2006,39(8):1536-1544.
[17] 王国英.基因工程实验技术[M].北京:中国农业科技出版,1997:43-46.
[18] Huang X,Kempf H,Ganal M,et al.Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat(Triticum aestivum L.)[J].Theoretical and Applied Genetics,2004,109(5):933-943.
[19] Mccartney C A,Somers D J,Humphreys D G,et al.Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL4452×AC Domain[J].Genome,2005,48(5):870-883.
[20] Wang R,Hai L,Zhang X,et al.QTL mapping for grain filling rate and yield-related traits in RILs of the Chinese winter wheat population Heshangmai×Yu8679[J].Theoretical and Applied Genetics,2009,118(2):313-325.
[21] 常成,张海萍,张秀英,等.小麦PEBP-like基因等位变异与籽粒大小、粒重关系研究[J].分子植物育种,2009,7(1):23-27.
[22] Sun X Y,Wu K,Zhao Y,et al.QTL analysis of kernel shape and weight using recombinant inbred lines in wheat[J].Euphytica,2008,165(3):615-624.

Please choose a citation manager

Content to export