Screening and Detection of Resistance to Phytophthora Blight Gene in Pepper Using Molecular Markers

doi:10.7668/hbnxb.20191405

Abstract

Abstract: In order to speed up the selection and breeding new varieties of resistance to Phytophthora blight peppers,breeding resistant varieties is an effective way to prevent or reduce the damage of pepper disease.With the development of molecular biology,molecular marker-assisted breeding of pepper has become a fast and effective method for breeding resistant varieties.The genes of resistance to Phytophthora bligh twere identified in 40 pepper materials by molecular marker technology,and 6 molecular markers of resistance to Phytophthora blight were screened at the same time.The identification results showed that three STS molecular markers Phy-1,Phy-4 and Phy-6 had target strips,and a total of 18 materials of resistance to Phytophthora blight and 2 susceptible materials were identified.Among them,14 disease-resistant materials were screened by Phy-1,7 disease-resistant materials were screened by Phy-4. 3 materials containing 2 disease-resistant genes,and 2 disease-susceptible materials was screened by Phy-6.Using molecular markers to screen disease-resistant resources can effectively improve the process of molecular breeding of disease-resistant in pepper and lay a foundation for accelerating the breeding of new disease-resistant varieties and industrial development of pepper.

Key words: Pepper, Phytophthora blight, Screening, Detection, Molecular marker

CLC Number:

S432.4
Q78

MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. Screening and Detection of Resistance to Phytophthora Blight Gene in Pepper Using Molecular Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341. doi: 10.7668/hbnxb.20191405.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20191405

http://www.hbnxb.net/EN/Y2020/V35/IS1/338

References

[1] Parra G,Ristaino J B.Resistance to mefenoxam and metalaxyl among fieldi solates of Phytophthora blight of bell pepper[J]. Plant Dis,2001,85:1069-1075.doi:10.1094/PDIS.2001.85.10.1069.
[2] Obata T S,Witt S,Lisec J,Palacios R N,Florez S I,Yousfi S.Metabolite profiles of maize leaves in drough the atand combined stress field trials reveal there lation ship between metabolism and grainyield[J]. Plant Physiol,2015,169:2665-2683.doi:10.1104/pp.15.01164.
[3] Wang J E,Li D W,Zhang Y L,Zhao Q,He Y M,Gong Z H.Defence responses of pepper(Capsicum annuum L.)infected with in compatible and compatible strains of Phytophthora capsici[J]. Plant Pathol,2013,136:625-638.doi:10.1007/s10658-013-0193-8.
[4] Reyes T,Alfredo,Castro R,Arturo,Rodrguez A,Gerardo P S,Martha E,Lamour K L,John F P,Sylvia P.Virulence phenotypes on chili pepper for Phytophthora capsici isolates from Michoacán Mexico[J]. Hort Science,2019,54(9):1526-1531.doi:10.21273/HORTSCI13964-19.
[5] Wang P Y,Wang L R,Guo J J,Yang W C,Shen H L.Molecular mapping of agene conferring resistance to Phytophthora capsici Leonian race 2 inpepper line PI201234(Capsicum annuum L.)[J]. Mol Breeding,2016,36:66.doi:10.1007/s11105-009-0178-0.
[6] Liu W Y,Kang J H,Jeong H S,Choi H J,Yang H B,Kim K T,Choi D,Choi G J,Jahn M,Kang B C.Combined use of bulked segregant analysis and microarrays reveals SNP markers pinpointing a major QTL for resistance to Phytophthora capsici in pepper[J]. Theor Appl Genet,2014,127(11):2503-2513.doi:10.1007/s00122-014-2394-8.
[7] 刁卫平,王述彬,刘金兵,潘宝贵,郭广君,戈伟,高长洲.辣椒疫病抗性相关,NAC基因的鉴定及表达分析[J].分子植物育种,2018(16):5183-5191.doi:10.13271/j.mpb.016.005183.
Diao W P,Wang S B,Liu J B,Pan B G,Guo G J,Ge W,Gao C Z.Identification and expression analysis of NAC gene related to phytoph-thora blight resistance in pepper[J]. Molecular Plant Breeding, 2018(16):5183-5191.
[8] 王博,左星,李永新,巩振辉,李大伟.辣椒抗疫病相关基因的RGA-STS标记的开发[J].西北农业学报,2010,19(10):124-127.
Wang B,Zuo X,Li Y X,Gong Z H,Li D W.Development of a sequence tagged sites marker derived from resistance gene analog(RGA-STS)associated with resistance to Phytophthora capsici in pepper(Capsicum annuum L.)[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010,19(10):124-127.
[9] Muhammad A Z,Muhammad S U H,Mukhtar A,Abdul M K,Kashif A,Hidayat U,Abid K Gang,Lu Z H.The Ca ChiVI2 gene of Capsicum annuum L.confers resistance agains the at stress and in fection of Phytophthora capsici[J]. Frontiersin Plant Science,2020(19):1-16.doi:10.3389/fpls.2020.00219.
[10] 王博,李永新,巩振辉,李大伟.辣椒疫病抗性相关的共显性RGA-STS标记的开发及应用[J].西北农业学报,2011,20(6):123-127.
Wang B,Li Y X,Gong Z H,Li D W.Development of a sequence tagged sites marker derived from resistancegene analog(RGA-STS)associated with resistance to Phytophthora capsici in pepper(Capsicum annuum L.)[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2011,20(6):123-127.
[11] 巩振辉,刘珂珂,逯明辉,李大伟.一种利用分子标记淘汰辣椒感疫病材料的方法,中国,ZL200910021240.9[P].2011-04-06.
Gong Z H,Liu K K,Lu M H,Li D W.a method of using molecular markers to eliminate the pestilential materials of pepper,China,ZL200910021240.9[P].2011-04-06.
[12] Qririn E A,Ogundiwin E A,Prince J P.Development of sequence characterized amplified region(SCAR)primers for the detection of Phyto.5.2 a major QTL for resistance to Phytophtho racapsici Leoninpepper[J]. Theor Appl Genet,2005(110):605-612.doi:10.1007/s00122-004-1874-7.
[13] 王得元,安康,李颖.一种辣椒抗疫病育种的分子标记辅助选择方法[P].中国专利:CN1970790,2007-05-30.
Wang D Y,An K,Li Y.A molecular marker assisted selection method for pepper disease resistance breeding[P].Chinese patent:CN1970790,2007-05-30.
[14] 马寿宾,孙艳,王琛.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013(10):107-110.
Ma S B,Sun Y,Wang C.Molecular marker assisted selection of pepper materials with resistance to Phytophthora blight[J].Northern Horticulture,2013(10):107-110.
[15] Pflieger S,Palloix A,Caranta C.Defense response genescolocalize with quantitative disease resistance loci in pepper[J]. Theor Appl Genet,2001(103):920-929.doi:10.1007/s001220100726.
[16] Thabuis A,Lefebvre V,Bernard G.Phenotypic and molecular evaluation of a recurrent selection program for a polygenic resistance to Phytophthora capsici in pepper[J]. Theor Appl Genet,2004(109):342-351.doi:10.1007/s00122-004-1633-9.
[17] Thabuis A,Palloix A,Pflieger S.Comparative mapping of Phytophthora resistance loci in pepper germplasm:evidence forconserved resistance loci across solanceae and for a large genetic diversity[J]. Theor Appl Genet,2003(106):1473-1485.doi:10.1007/s00122-003-1206-3.
[18] 张晓芬,韩华丽,陈斌,耿丽华,耿三省.甜椒疫病抗性遗传及相关基因分子标记研究[J].园艺学报,2011,38(7):1325-1332.
Zhang X F,Han H L,Chen B,Geng L H,Geng S S.Genetic and molecular marker analysis of resistance to Phythophtora capsici in sweet pepper[J].Acta Horticulturae Sinica,2011,38(7):1325-1332.
[19] Kim B S,Kwon Y S.Inheritance of resistance to bacterial spot and Phytophhora blight in pepper[J]. Journal of Korean Society for Horticultural Science,1991,7:17-24.doi:10.1590/S1982-56762010000300002.
[20] 张爱民,蓬桂华,梁传静,袁圆,熊世娟,涂祥敏,王兰.贵州地方辣椒材料疫病抗性鉴定的分子标记引物筛选[J].贵州农业科学,2017,45(11):10-14.
Zhang A M,Peng G H,Liang C J,Yuan Y,Xiong S J,Tu X M,Wang L.Molecular marker primers screening of identification on local pepper with Phytophthora blight resistance in Guizhou[J].Guizhou Agricultural Sciences,2017,45(11):10-14.

Please choose a citation manager

Content to export