Improving Seedling Blast Resistance of Rice CMS Line Taonong1A Through Marker-assisted Selection of the Pigm Gene

doi:10.7668/hbnxb.20194645

Abstract

Abstract:

To improve seedling blast resistance of rice cytoplasmic male sterile(CMS)line Taonong1A and its maintainer Taonong1B,the Chinese local rice variety Gumei 4 was used as the donor parent harboring the broad-spectrum and durable blast resistance gene Pigm,and the Pigm-tightly-linked codominant marker T9E3 was developed and employed in marker-assisted selection(MAS)breeding practice.T9E3 showed clear and stable polymorphism between the donor and acceptor parents,amplifying a 926 bp DNA band from Gumei 4 genome,while the PCR product from either genome of Taonong1B or Taonong1A was about 2 000 bp.Greenhouse inoculation assay was performed using thirty-two Magnaporthe oryzae isolates collected from different rice areas,Gumei 4 and the improved rice lines showed much higher resistance frequency(90.63%)than that of two acceptor parents Taonong1B and Taonong1A(9.38%).Accordingly,Gumei 4 and the improved rice lines showed high-level seedling blast resistance(0 grade)but highly susceptible(8 grade)for two acceptor parents in natural nursery.Three blast resistant CMS pure lines(Taonong1A-Pigm-1—Taonong1A-Pigm-3)and the corresponding resistant maintainers(Taonong1B-Pigm-1—Taonong1B-Pigm-3)were preliminarily bred through MAS breeding,and one pair of them,Taonong1A-Pigm-2/Taonong1B-Pigm-2,was screened for potential application duo to the high-level blast resistance and elite sterility,agronomic and yield traits.Taonong1A-Pigm-2 showed complete male sterility with nearly 60% typical abortive pollens and 40% round abortive pollens,higher stigma exsertion rate(71.1%)and lower enclosed spikelet rate(33.6%),Taonong1B-Pigm-2 exhibited excellent agronomic and yield trait indexes,such as moderate sowing-heading duration(69 days),plant height(76.2 cm),main panicle length(28.1 cm),panicles per plant(15.8),spikelets per panicle(115.3)and 1000-grain weight(27.7 g)。New parent materials were created for the application of cytoplasmic male sterile(CMS)line hybrid rice breeding,and the comprehensive improvement of seedling blast resistance and characters of Taonong1A and Taonong1B were realized.

Key words: Rice, Rice blast resistance improvement, Molecular marker-assisted selection, Pigm, Taonong1A, Taonong 1B

ZHOU Geng, HUANG Jun, ZOU Yuying, DENG Jiqi, LI Jiaxin, CHEN Qiang, GUO Chenglong, LI Bowen, CHE Fanhao, YAO Wei, HUANG Xilai, LIU Jinling, LIU Xionglun. Improving Seedling Blast Resistance of Rice CMS Line Taonong1A Through Marker-assisted Selection of the Pigm Gene[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 39-46. doi: 10.7668/hbnxb.20194645.

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Figures/Tables 8

References 29

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菌株 Isolates	来源 Origin	CO39 (CK)	谷梅4号 Gumei 4	桃农1B Taonong1B	桃农1A Taonong1A	桃农1 B-Pigm Taonong 1B-Pigm	桃农1 A-Pigm Taonong 1A-Pigm
CHL1743	广东	S	R	S	S	R	R
21GX003	广西	S	R	S	S	R	R
21ZJ120	浙江	S	R	S	S	R	R
21ZJ126	浙江	S	R	R	R	R	R
CHL645	贵州	S	R	S	S	R	R
E2007046A2	湖北	S	S	S	S	S	S
RB20	福建	S	R	S	S	R	R
21ZJ004	福建	S	R	S	S	R	R
21XX090	福建	S	R	R	R	R	R
21SC004	四川	S	R	S	S	R	R
110-2	湖南	S	R	S	S	R	R
195-2-2	湖南	S	R	S	S	R	R
X2007A-1	湖南	S	R	S	S	R	R
X2007A-3	湖南	S	R	S	S	R	R
X2007A-7	湖南	S	R	S	S	R	R
318-2	湖南	S	R	S	S	R	R
21XX051	湖南	S	R	S	S	R	R
21ZZ01	湖南	S	R	S	S	R	R
CHL441	湖南	S	S	S	S	S	S
236-1	湖南	S	R	S	S	R	R
21XX062	湖南	S	R	S	S	R	R
KJ201	韩国	S	R	S	S	R	R
ROR1	韩国	S	R	S	S	R	R
KOH	日本	S	R	S	S	R	R
GUY11	法国	S	R	S	S	R	R
P06-b	菲律宾	S	R	S	S	R	R
95097AZC13	中国	S	R	S	S	R	R
RB10	中国	R	R	R	R	R	R
CHL1755	中国	S	R	S	S	R	R
YW538	中国	S	R	S	S	R	R
RB11	中国	S	S	S	S	S	S
che86061	中国	S	R	S	S	R	R
抗性频率/%		3.13	90.63	9.38	9.38	90.63	90.63
Resistance frequency

菌株 Isolates	来源 Origin	CO39 (CK)	谷梅4号 Gumei 4	桃农1B Taonong1B	桃农1A Taonong1A	桃农1 B-Pigm Taonong 1B-Pigm	桃农1 A-Pigm Taonong 1A-Pigm
CHL1743	广东	S	R	S	S	R	R
21GX003	广西	S	R	S	S	R	R
21ZJ120	浙江	S	R	S	S	R	R
21ZJ126	浙江	S	R	R	R	R	R
CHL645	贵州	S	R	S	S	R	R
E2007046A2	湖北	S	S	S	S	S	S
RB20	福建	S	R	S	S	R	R
21ZJ004	福建	S	R	S	S	R	R
21XX090	福建	S	R	R	R	R	R
21SC004	四川	S	R	S	S	R	R
110-2	湖南	S	R	S	S	R	R
195-2-2	湖南	S	R	S	S	R	R
X2007A-1	湖南	S	R	S	S	R	R
X2007A-3	湖南	S	R	S	S	R	R
X2007A-7	湖南	S	R	S	S	R	R
318-2	湖南	S	R	S	S	R	R
21XX051	湖南	S	R	S	S	R	R
21ZZ01	湖南	S	R	S	S	R	R
CHL441	湖南	S	S	S	S	S	S
236-1	湖南	S	R	S	S	R	R
21XX062	湖南	S	R	S	S	R	R
KJ201	韩国	S	R	S	S	R	R
ROR1	韩国	S	R	S	S	R	R
KOH	日本	S	R	S	S	R	R
GUY11	法国	S	R	S	S	R	R
P06-b	菲律宾	S	R	S	S	R	R
95097AZC13	中国	S	R	S	S	R	R
RB10	中国	R	R	R	R	R	R
CHL1755	中国	S	R	S	S	R	R
YW538	中国	S	R	S	S	R	R
RB11	中国	S	S	S	S	S	S
che86061	中国	S	R	S	S	R	R
抗性频率/%		3.13	90.63	9.38	9.38	90.63	90.63
Resistance frequency

水稻品系 Rice line	播始历期/d Sowing- heading duration	株高/cm Plant height	剑叶长宽比 Flag leaf length- width ratio	主穗穗长 /cm Main panicle length	单株有效穗数 Panicles per plant	着粒密度 /(粒/cm) Spikelet density	单穗总颖花数 Spikelets per panicle	千粒质量/g 1000-grain weight	结实率/% Seed setting rate	单株理论产量/g Theoretical yield per plant
桃农1B Taonong1B	70	78.3±0.59bc	22.4±0.93a	26.2±0.53b	14.9±0.74a	3.9±0.14a	101.4±3.31b	24.3±0.41d	77.3±1.03a	28.4±1.36c
桃农1B-Pigm-1 Taonong1B-Pigm-1	69	78.7±2.11b	22.3±0.74a	26.3±0.67b	14.8±1.62a	4.2±0.26a	111.6±7.53a	26.7±0.23b	77.4±1.54a	34.1±4.26b
桃农1B-Pigm-2 Taonong1B-Pigm-2	69	76.2±2.13c	22.8±0.56a	28.1±1.20a	15.8±1.40a	4.1±0.43a	115.3±11.26a	27.7±0.19a	78.3±1.30a	39.5±5.29a
桃农1B-Pigm-3 Taonong1B-Pigm-3	69	83.0±1.45a	22.8±0.82a	27.5±0.81a	15.4±1.35a	4.1±0.24a	113.4±5.91a	26.3±0.05c	77.8±1.04a	35.8±4.12ab
桃农1A Taonong1A	73	66.0±1.05a	22.5±0.84a	26.3±0.62c	14.7±0.48b	4.2±0.25a	110.2±5.69b	—	—	—
桃农1A-Pigm-1Taonong1A-Pigm-1	71	66.1±1.51a	22.0±0.74a	26.4±0.59c	15.9±1.29ab	4.5±0.23a	119.0±5.68ab	—	—	—
桃农1A-Pigm-2 Taonong1A-Pigm-2	72	65.3±1.85a	21.7±1.18a	29.3±0.86a	17.2±0.79a	4.4±0.33a	125.7±7.66a	—	—	—
桃农1A-Pigm-3 Taonong1A-Pigm-3	71	67.2±1.26a	22.4±0.76a	27.5±0.78b	16.1±1.20ab	4.4±0.28a	120.3±6.48a	—	—	—

水稻品系 Rice line	播始历期/d Sowing- heading duration	株高/cm Plant height	剑叶长宽比 Flag leaf length- width ratio	主穗穗长 /cm Main panicle length	单株有效穗数 Panicles per plant	着粒密度 /(粒/cm) Spikelet density	单穗总颖花数 Spikelets per panicle	千粒质量/g 1000-grain weight	结实率/% Seed setting rate	单株理论产量/g Theoretical yield per plant
桃农1B Taonong1B	70	78.3±0.59bc	22.4±0.93a	26.2±0.53b	14.9±0.74a	3.9±0.14a	101.4±3.31b	24.3±0.41d	77.3±1.03a	28.4±1.36c
桃农1B-Pigm-1 Taonong1B-Pigm-1	69	78.7±2.11b	22.3±0.74a	26.3±0.67b	14.8±1.62a	4.2±0.26a	111.6±7.53a	26.7±0.23b	77.4±1.54a	34.1±4.26b
桃农1B-Pigm-2 Taonong1B-Pigm-2	69	76.2±2.13c	22.8±0.56a	28.1±1.20a	15.8±1.40a	4.1±0.43a	115.3±11.26a	27.7±0.19a	78.3±1.30a	39.5±5.29a
桃农1B-Pigm-3 Taonong1B-Pigm-3	69	83.0±1.45a	22.8±0.82a	27.5±0.81a	15.4±1.35a	4.1±0.24a	113.4±5.91a	26.3±0.05c	77.8±1.04a	35.8±4.12ab
桃农1A Taonong1A	73	66.0±1.05a	22.5±0.84a	26.3±0.62c	14.7±0.48b	4.2±0.25a	110.2±5.69b	—	—	—
桃农1A-Pigm-1Taonong1A-Pigm-1	71	66.1±1.51a	22.0±0.74a	26.4±0.59c	15.9±1.29ab	4.5±0.23a	119.0±5.68ab	—	—	—
桃农1A-Pigm-2 Taonong1A-Pigm-2	72	65.3±1.85a	21.7±1.18a	29.3±0.86a	17.2±0.79a	4.4±0.33a	125.7±7.66a	—	—	—
桃农1A-Pigm-3 Taonong1A-Pigm-3	71	67.2±1.26a	22.4±0.76a	27.5±0.78b	16.1±1.20ab	4.4±0.28a	120.3±6.48a	—	—	—

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