Effects of Low Temperature Stress at Flowering Stage on Ultra Structure of Pollen Grains and Leaf of Rice in Cold Region

doi:10.7668/hbnxb.20193881

Abstract

Abstract:

In order to study the effect of low temperature on the ultrastructure of pollen grains and flag leaves of rice in cold regions,Japonica rice varieties Kongyu 131 and Longjing 25 with strong cold tolerance and varieties Longjing 11 and Longjing 28 with poor cold tolerance were selected as research materials in cold regions,low temperature treatment during flowering period (13,15,17 ℃),the samples were observed and photographed using scanning and transmission electron microscopy.The results showed that:Under low temperature stress,the reticular patterns on the pollen grains of Kongyu 131,Longjing 11 and Longjing 25 changed significantly,the reticular carvings on the surface of rice pollen grains changed from deep to shallow,with the decrease of stress temperature,the reticular carvings on the surface of rice pollen grains changed from deep to shallow,reduction of surface protrusions,the surface of pollen grains became smooth,which was not conducive to the attachment of pollen on stigma because made the surface of pollen grains smooth;however,the pollen grain surface characteristics of Longjing 28 under low temperature stress were not significantly different from those of the control.With the decrease of temperature,starch grains in chloroplasts of rice varieties decreased significantly,osmiophilic grains increased significantly,grana became loose,and the proportion of superposed thylakoid membranes decreased;however,under the influence of low temperature,the varieties Longjing 25 and Kongyu 131 with strong cold tolerance and the varieties Longjing 11 and Longjing 28 with poor cold tolerance did not show the same law in grana lamella and matrix lamella,while the changes in starch grain synthesis and osmiophilic particle number were consistent.Compared with rice pollen grains,the effect of low temperature on sword leaf ultrastructure was more obvious. Under low temperature stress,leaf function declined and chloroplast photosynthetic changed among rice varieties with strong and poor cold tolerance were significantly different,with obvious rules.

Key words: Japonica rice in cold region, Cold resistance, Cells, Ultrastructure

DU Xiaodong, CAI Yongsheng, ZHAO Haixin, CHEN Shuqiang, XU Lingqi, YANG Limin. Effects of Low Temperature Stress at Flowering Stage on Ultra Structure of Pollen Grains and Leaf of Rice in Cold Region[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 78-85. doi: 10.7668/hbnxb.20193881.

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Figures/Tables 9

Tab.1 Cold resistant treatment numbers

编号 Number	处理 Handle	品种 Variety	处理温度/℃ Temperature
1	1-1	空育131	CK
2	1-2	空育131	13
3	1-3	空育131	17
4	2-1	龙粳11	CK
5	2-2	龙粳11	13
6	2-3	龙粳11	17
7	3-1	龙粳25	CK
8	3-2	龙粳25	13
9	3-3	龙粳25	17
10	4-1	龙粳28	CK
11	4-2	龙粳28	13
12	4-3	龙粳28	17
13	T1	冷灌空育131	15
14	T2	冷灌龙粳11	15
15	T3	冷灌龙粳25	15
16	T4	冷灌龙粳28	15

Fig.1 Scanning characteristics of Kongyu 131 pollen grains at different temperatures A.CK(Under natural conditions);B.At 13 ℃;C.At 17 ℃;D.Under the condition of field cold irrigation.The same as Fig.2—4.

Fig.2 Scanning characteristics of Longjing 11 pollen grains at different temperatures

Fig.3 Scanning characteristics of Longjing 25 pollen grains at different temperatures

Fig.4 Scanning characteristics of Longjing 28 pollen grains at different temperatures

Fig.5 Chloroplast ultrastructure of Kongyu 131 at different temperatures A.CK(Under natural conditions);B.At 13 ℃;C.At 17 ℃.The same as Fig.6—8.

Fig.6 Chloroplast ultrastructure of Longjing 11 at different temperatures

Fig.7 Chloroplast ultrastructure of Longjing 25 at different temperatures

Fig.8 Chloroplast ultrastructure of Longjing 28 at different temperatures

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