Curcumin Improves TGF-&#x003B2;1-induced BEND Epithelial-Mesenchymal Transition by Promoting Autophagy

doi:10.7668/hbnxb.20195408

Abstract

Abstract:

To study the mechanism of curcumin(CUR)improving epithelial-mesenchymal transition(EMT)of bovine endometrial epithelial cells(BEND)induced by transforming growth factor-β1(TGF-β1)by regulating autophagy,different concentrations of TGF-β1(2.5,5.0 and 10.0 ng/mL)were used to induce the EMT model.Western Blot was used to detect the effects of different concentrations of curcumin(5,10 and 20 μmol/L)on the expression of the EMT-related proteins and autophagy-related proteins in BEND induced by TGF-β1.The expression levels of the EMT-related proteins(E-cadherin,N-cadherin and α-SMA)were detected by immunofluorescence.BEND was treated with autophagy inhibitor chloroquine(CQ)(10 μmol/L)to detect the expression of the EMT-related proteins and autophagy-related proteins.The expression levels of E-cadherin,Beclin-1 and LC3 proteins in BEND induced by TGF-β1 were significantly decreased,and the expression levels of N-cadherin,α-SMA and P62 proteins were significantly increased.Curcumin treatment could significantly increase the expression levels of E-cadherin,Beclin-1 and LC3 proteins,and significantly reduce the expression levels of N-cadherin,α-SMA and P62 proteins.Compared with curcumin treatment,CQ treatment significantly increased the expression levels of N-cadherin,α-SMA,P62 and LC3 proteins,and significantly decreased the expression levels of E-cadherin and Beclin-1 proteins.Curcumin can improve TGF-β1 induced BEND EMT function by promoting autophagy.

Key words: Curcumin, TGF-β1, Bovine endometrial epithelial cells, Autophagy, Epithelial-mesenchymal transition

CLC Number:

R285.5

WANG Linnan, LI Xiangchen, WANG Guanqing, TU Jiusu, YANG Yuqiu, SONG Liangli. Curcumin Improves TGF-β1-induced BEND Epithelial-Mesenchymal Transition by Promoting Autophagy[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 224-230. doi: 10.7668/hbnxb.20195408.

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Figures/Tables 4

Fig.1 The effects of TGF-β1 on the expression of EMT and autophagy-related proteins in BEND A—B.EMT-related proteins expression;C—D.Autophagy-related proteins expression. Different lowercase letters showed significant difference(P<0.05).The same as Fig.2,4.

Fig.2 The effects of curcumin(CUR)on the expression of EMT and autophagy-related proteins in BEND induced by TGF-β1 A—B.EMT-related proteins expression; C—D.Autophagy-related proteins expression.

Fig.3 The effect of curcumin on the expression of BEND EMT-related proteins induced by TGF-β1 was detected by immunofluorescence Immunofluorescence expression of EMT-related proteins (400×).

Fig.4 The mechanism of curcumin on TGF-β1-induced BEND EMT

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