The Mechanism of DL-Methionine Methylsulfonium Chloride Alleviating Lipopolysaccharide-Induced Apoptosis in IPEC-J2 Cells

doi:10.7668/hbnxb.20195572

Abstract

Abstract:

This study aimed to investigate the effect of DL-methionine methylsulfonium chloride(MMC)on apoptosis and the expression of related protein genes in lipopolysaccharide(LPS)-induced porcine jejunal epithelial cells(IPEC-J2),and to elucidate its potential mechanism of action.By setting up a blank group,a model group(LPS-treated)and an experimental group(MMC+LPS-treated),the study compared the effects of MMC on the viability,apoptosis rate,migration number and apoptosis-related proteins of LPS-induced IPEC-J2 cells.It was found that the experimental group significantly increased cell activity by 18% and migration number by about 2 times,and significantly decreased apoptosis rate by 40% compared with the model group.Additionally,MMC also inhibited LPS-induced activation of the JNK pathway,as evidenced by a highly significant decrease of 25.5% in the p-JNK protein expression level in the LPS model group.Further analysis showed that MMC significantly reduced the expression of pro-apoptotic proteins Cleaved-caspase-3 and BAX,which decreased by 31.5% and 26.3% respectively in the LPS model group,while it markedly increased the expression level of the anti-apoptotic protein BCL-2.In conclusion,MMC effectively alleviates LPS-induced apoptosis in IPEC-J2 cells by regulating the expression of apoptosis-related proteins and inhibiting JNK pathway activation,which provides a scientific basis for the application of MMC in protecting intestinal health in pigs.

Key words: DL-methionine methylsulfonium chloride, Lipopolysaccharide, IPEC-J2 cells, Cell viability, Cell apoptosis

CLC Number:

S816.71无机(矿物质)饲料

JIANG Dongfeng, QIAO Yingying, YANG Jianping, NIE Furong, LI Rui, WANG Ke, LEI Lei, ZHANG Weixian. The Mechanism of DL-Methionine Methylsulfonium Chloride Alleviating Lipopolysaccharide-Induced Apoptosis in IPEC-J2 Cells[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 232-238. doi: 10.7668/hbnxb.20195572.

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Figures/Tables 7

Fig.1 The effect of MMC on the viability of LPS-induced IPEC-J2 cells Different uppercase letters indicate significant differences at the 0.01 level,different lowercase letters indicate significant differences at the 0.05 level.The same as Fig.3,5,6,7.

Fig.2 Flow cytometry analysis of the effect of MMC on apoptosis in LPS-induced IPEC-J2 cells A.Blank group;B.LPS model group;C.MMC+LPS treatment group.

Fig.3 The effect of MMC on apoptosis in LPS-induced IPEC-J2 cells

Fig.4 Migration analysis of IPEC-J2 cells induced by LPS and treated with MMC A.Blank group;B.LPS model group;C.MMC+LPS treatment group.

Fig.5 The effect of MMC on the migration of LPS-induced IPEC-J2 cells

Fig.6 The effect of different treatments on JNK and p-JNK expression in IPEC-J2 cells 1.Blank group;2.LPS model group;3.MMC+LPS treatment group.The same as Fig.7.

Fig.7 The effect of different treatments on the expression of apoptosis-related proteins

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