Effect of Baicalin on Oxidative Damage of Mouse Oviduct Induced by Heat Stress

doi:10.7668/hbnxb.2017.06.022

Abstract

Abstract: In order to reduce the heat stress caused by animal body reactive oxygen species (ROS) increased, reducing tissue oxidative damage and cell apoptosis.The effect of heat stress on the oxidative damage of oviduct tissues was studied by using the mouse model, and the protective effect of baicalin on the oxidative damage of the oviduct was investigated. The mice were divided into control(group Ⅰ), Bai(group Ⅱ),41℃ heat stress (groupⅢ) and 41℃ heat stress+Bai (group Ⅳ). Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were detected by kits, respectively. The expression of Nrf2, Keap1, HO-1 and NQO1 proteins were detected by using Western Blot and the expressions of Apaf-1 and Caspase-9 were detected by immunohistochemistry method. The results showed that compared with the control group, the content of MDA in oviduct tissue was significantly increased (P<0.05) and the activities of CAT and GSH-Px were significantly decreased after heat stress.The expression of Nrf2 and Keap1 proteins were significantly increased(P<0.05). Also Apaf-1 and Caspase-9 proteins were significantly increased as compared with control group. However, compared with group Ⅲ, baicalin significantly (P<0.05) decreased the content of MDA and the expression of Nrf2 and Keap1 proteins. The apoptotic related proteins of Apaf-1 and Caspase-9 were also decreased. The activities of SOD, CAT and GSH-Px increased significantly (P<0.05).These results indicate that baicalin can reduce the oxidative damage and apoptosis of oviduct tissue of mouse under heat stress, and its mechanism may be related to activation of antioxidant signaling pathway of Keap1-Nrf2/ARE.

Key words: Baicalin, Heat stress, Oviduct, Oxidative damage, Cell apoptosis

CLC Number:

WANG Xue, JIANG Zhongling, WANG Xin, JIANG Aimin, CONG Xia, CAO Rongfeng, LI Huatao, TIAN Wenru. Effect of Baicalin on Oxidative Damage of Mouse Oviduct Induced by Heat Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 147-153. doi: 10.7668/hbnxb.2017.06.022.

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References

[1] Stecyk J A, Couturier C S, Fagernes C E, et al. Quantification of heat shock protein mRNA expression in warm and cold anoxic turtles (Trachemys scripta) using an external RNA control for normalization[J]. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 2012, 7(1):59-72.
[2] Hansen P J. Genetic variation in resistance of the preimplantation bovine embryo to heat shock[J]. Reproduction Fertility and Development, 2014, 27(1):22-30.
[3] Tegethoff V, Hartung J. Animal housing climatic quality in various harmful conditions in broiler production to avoid heat stress in summer[J]. Deutsche Tierarztliche Wochenschrift, 1996, 103(3):87-91.
[4] Hansen P J. Cellular and molecular basis of therapies to ameliorate effects of heat stress on embryonic development in cattle[J]. Animal Reproduction, 2013, 10(3):322-333.
[5] Gupta S, Agarwal A, Banerjee J, et al. The role of ox-idative stress in spontaneous abortion and recurrent preg-nancy loss:a systematic review[J]. Obstetrical & Gynecological Survey, 2007, 629(5):335-347.
[6] Qian Y, Shi W Q, Ding J T, et al. Effects of type and state of co-culture cells on in-vitro development of porcine oocytes matured and fertilized in vitro[J]. Journal of Assisted Reproduction and Genetics, 2005, 22(6):233-238.
[7] 孙先枝,程建波,卜登攀,等.黄芩苷的生物学功能和黄芩及其提取物在畜禽生产中的应用研究进展[J].动物营养学报, 2013(7):1459-1464.
[8] 孙春玲,国晓瞳,赵园,等.黄芩苷对热应激条件下猪近端肾小管(LLC-PK1)细胞凋亡率及B细胞淋巴瘤-2基因(Bcl-2)和Bcl-2 相关X蛋白基因(Bax)表达的影响[J].农业生物技术学报, 2014,22(12):1553-1560.
[9] 关翠雯,金晶,李佳,等.丹参酮ⅡA激活Nrf2/ARE通路保护雷公藤甲素所致急性肝损伤[J].药学学报, 2013(9):1397-1402.
[10] Corsi M M, Fulgenzi A, Gaja G, et al. Effect of acute and chronic inflammation on plasma growth hormone levels in rats[J]. Drugs Under Experimental and Clinical Research, 1997, 23(3/4):117-122.
[11] Valls-Belles V, Torres Mdel C, Boix L.Alpha-Tocopherol, MDA-HNE and 8-OHd G levels in liver and heart mitochondria of adriamycin-treated rats fed with alcohol-free beer[J]. Toxicology,2008,249(213):97-101.
[12] Lu X T, Yu C. Enantiomer-specific profenofos-induced cytotoxicity and DNA damage mediated by oxidative stress in rat adrenal pheochromocytoma (PC12) cells[J]. Journal of Applied Toxicology,2014,34(2):166-175.
[13] 国晓瞳,张倩,于枫,等.黄芩苷对热应激犊牛睾丸支持细胞胶质细胞源性神经生长因子(GDNF)和干细胞因子(SCF)表达的影响[J].农业生物技术学报, 2015(4):441-449.
[14] Yang X, Yao W, Shi H, et al. Paeoniflorin protects Schwann cells against high glucose induced oxidative injury by activating Nrf2/ARE pathway and inhibiting apoptosis[J]. Journal of Ethnopharmacology, 2016, 185:361-369.
[15] Meng H X, Guo J H, Wang H F, et al. Erythropoietin activates Keap1-Nrf2/ARE pathway in rat brain after ischemia[J]. International Journal of Neuroscience, 2014, 124(5):362-368.
[16] Tong K I, Katoh Y, Kusunoki H, et al. Keap1 recruits Neh2 through binding to ETGE and DLG motifs:characterization of the two-site molecular recognition model[J]. Molecular and Cellular Biology, 2006, 26(8):2887-2900.
[17] Wakabayashi N, Dinkova-Kostova A T, Holtzclaw W D, et al. Protection against electrophile and oxidant stress by induction of the phase 2 response:fate of cysteines of the Keap1 sensor modified by inducers[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(7):2040-2045.
[18] Li L, Dong H, Song E, et al. Nrf2/ARE pathway activation, HO-1 and NQO1 induction by polychlorinated biphenyl quinone is associated with reactive o xygen species and PI3K/AKT signaling[J]. Chemico-Biological Interactions, 2014, 209(1):56-67.
[19] Draheim T, Liessem A, Scheld M, et al. Activation of the astrocytic Nrf2/ARE system ameliorates the formation of demyelinating lesions in a multiple sclerosis animal model[J]. Glia, 2016, 64(12):2219-2230.
[20] Jiang T, Huang Z, Lin Y, et al. The protective role of Nrf2 in streptozotocin-induced diabetic nephropathy[J]. Diabetes, 2010, 59(4):850-860.
[21] 庞纯,蒋萍,季莉莉.黄芩素激活核转录因子Nrf2拮抗肝毒性的研究[J].中国药理学通报, 2014, 30(4):546-549.
[22] Anichini A, Mortarini R, Sensi M, et al. APAF-1 signaling in human melanoma[J]. Cancer Letters, 2006, 238(2):168-179.

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