Effects of Nano-selenium on Serum Antioxidant and Immune Indexes of Oncorhynchus mykiss under Heat Stress

doi:10.7668/hbnxb.20192837

Abstract

Abstract:

In order to study the effects of nano-selenium on serum antioxidant and immune indexes of rainbow trout under heat stress,600 8-month-old rainbow trout with an average body weight of 80 g were selected and randomly divided into 3 groups:the control group was fed with the basal diet,and the experimental groups were fed with 5,10 mg/kg nano-selenium diets.The rainbow trout was fed at the suitable growth water temperature(18 ℃)for 9 days,At 24 ℃ (heat stress for rainbow trout)for 48 h.10 fish were randomly selected from each group before heat stress(18 ℃,0,3,6,9 d)and after heat stress(24 ℃,4,8,12,24,48 h)and serum was collected to determine antioxidant and immune indexes.The results showed that compared with the control group,the experimental group significantly increased the contents of TP,ALB and GLB in serum(P<0.05);the activity of GSH-Px and SOD were significantly decreased,as well as CAT and MDA were significantly increased in heat stress,but the addition of nano-selenium significantly increased the activity of GSH-Px,SOD,CAT and decreased MDA activity;the contents of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in serum of the control group were gradually increased by heat stress. The contents of TNF-α and IL-1β in serum of the 5 mg/kg nano-selenium group were significantly increased at 4 h, and the contents of IL-1β were significantly decreased at 12 h compared with the control group.The content of IgM decreased at 4 h and increased at 12 h after heat stress,and nano-selenium supplementation significantly increased the activity of IgM;after heat stress,the content of C3 decreased gradually,but the addition of nano-selenium significantly increased the activity of C3.In conclusion,dietary nano-selenium supplementation can improve the antioxidant capacity and immune capacity of rainbow trout under heat stress.Compared with 10 mg/kg nano-selenium,the antioxidant effect and immune effect of 5 mg/kg nano-selenium are more stable.

Key words: Rainbow trout, Nano-selenium, Heat stress, Antioxidant index, Immune index

LU Junhao, LI Lanlan, QUAN Jinqiang, ZHAO Guiyan, SUN Jun, LIN Qiaorong, LIU Zhe. Effects of Nano-selenium on Serum Antioxidant and Immune Indexes of Oncorhynchus mykiss under Heat Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 231-238. doi: 10.7668/hbnxb.20192837.

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Figures/Tables 4

Tab.1 Determination results of selenium content in feedmg/kg

编号 Sample	0 mg/kg			5 mg/kg			10 mg/kg
编号 Sample	1	2	3	1	2	3	1	2	3
硒含量 Selenium content	0.44	0.66	0.59	5.75	5.74	5.66	10.07	10.53	10.63

Fig 1. Effects of nano-selenium on serum protein content of rainbow trout under heat stress(n=10) Different lowercase letters indicate significant difference of enzyme activity between different treatment groups at the same time(P<0.05).The same as Fig.2,3.

Fig.2 Effects of nano-selenium on antioxidant enzymes in serum of rainbow trout under heat stress(n=10)

Fig.3 Effects of nano-selenium on immune related enzymes in rainbow trout serum under heat stress(n=10)

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