Polymorphism at 3'UTR of the Heat Shock Protein 70 Gene and Its Relationship with Thermal Tolerance in Chinese Holstein

doi:10.7668/hbnxb.2010.03.004

Abstract

Abstract: Heat shock protein 70( HSP70) gene was regarded as a candidate gene for thermo resistance of cows.In this study,a 549 bp fragment of HSP70 gene of Chinese Holstein was amplified by polymerase chain reaction( PCR), the polymorphisms of three loci of HSP70 gene were detected by PCR-SSCP technique.By sequencing, three point mutations, G/C at nucleotide 6 400,C/T at nucleotide 6 600 and G/A at nucleotide 6 601,were identified in HSP70 gene sequence.χ2 test indicated that three polymorphisms in the population unfitted with Hardy-Weinberg equilibrium( P <0.01).Meanwhile, the relationship between milk performance and heat tolerance was analyzed.The results showed that in the three loci, the different genotypes of HSP70 gene had no significant influence on milk protein percent and fat percent,but had significant influence on 305-day milk yield.Individuals with genotype BB showed higher 305-day milk yield than those with genotypes AA and CC( P <0.05).And the cows with genotype BB showed lower potassium content in erythrocytes ( PCE) and decrease rate of milk yield than those with genotypes AA and CC.It indicated that BB genotype was the advantageous genotype.These mutations could be used in MAS for anti-heat stress cows in our breeding program．

Key words: Holstein cows, HSP70 gene, Polymorphism, Heat stress, PCR-SSCP

CLC Number:

DU Fang-lei,, WANG Hong-mei, HUANG Jin-ming, LI Jian-bin, ZHONG Ji-feng, ZHANG Ting-rong, LIU Jian-fei, LI Qiu-ling, WANG Chang-fa. Polymorphism at 3'UTR of the Heat Shock Protein 70 Gene and Its Relationship with Thermal Tolerance in Chinese Holstein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 14-18. doi: 10.7668/hbnxb.2010.03.004.

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References

[1] Schwein M.Perspective of molecular genome analysis in livestock improvement an overview[J].J Anim Res Dev,1995,42:16-26.
[2] McComb M A,Spurlock ME．Expression of stress proteins in porcine tissues:Developmental changes and effect of immunological challenge[J].Anmi Sci,1997,75:195-201.
[3] Yahav S,Shamay A,Horev G,et al.Effect of acquisition of improved thermotolerance on the induction of heat shock proteins in broiler chickens[J].Poultry Science,1997,76:1428-1434.
[4] Mezquita B.Marked differences between avian and mammalian testicular cells in the heat-shock induction and polyadenylation of HSP70 and abiquitin transcripts[J].FEBS Letters,1998,436:382-386.
[5] Schwerin M,Maak S,Kalbe C.Functional promoter variants of highly conserved inducible HSP70 genes significantly affect stress response[J].Biophysica Acta,2001,1522(2):108-111.
[6] 王枫.热休克蛋白与热耐力研究进展[J].国外医学卫生学分册,1997,24(2);65-67.
[7] 鲍恩东,龚远英,Hartung J,等.肉鸡热应激病理损伤与应急蛋白(HSP70)相关性研究[J].中国农业科学,2004,37(2):301-305.
[8] Krobitsch S,Lindquist S.Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins[J].Proceedings of the National Academy of Science of the United States of America,2000,97(4):1589-1594.
[9] 程维杰,李秋玲,王洪梅,等.荷斯坦牛HSP70-1基因遗传多态性与乳腺炎抗性关系分析[J].遗传,2007,31(2):169-174.
[10] 陈强,李浩忠,王根林.奶牛HSP70基因多态性与生产性能的关系[J].江西农业学报,2007,19(7):84-86.
[11] Huang S Y,Chen M Y,Lin E C,et al.Effects of single nucleotide polymorphisms in the 5'flanking region of heat-shock protein 70.2 gene on semen qulity in boars[J].Animal Reproduction Science,2002,70:99-109.
[12] Tumer H G.Genetic variation of rectal temperature in cows and its relationship to fertility[J].Animal Production,1982,35:401-412.
[13] West J W.Effects of heat-stress on production in dairy science[J].Journal of Dairy Science,2003,86:2131-2144.

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