Analysis of ANO5 Gene Polymorphism and Its Association with Fat Deposition Traits in Hu Sheep

doi:10.7668/hbnxb.20194928

Abstract

Abstract:

Anoctamin 5(ANO5)is a multichannel membrane protein localized in the sarcoplasmic and sarcoplasmic reticulum that primarily plays a role in myosin membrane repair and phospholipid scrambling,mutations in the ANO5 gene can lead to jaw hypoplasia as well as various myopathies.It aimed to investigate the association of SNPs in the ANO5 gene with fat deposition traits in sheep.A population of 1 005 healthy and clearly genealogical Hu sheep male lambs was selected for the study,and PCR amplification and KASPar typing techniques were used to detect the locus polymorphisms of the ANO5 gene in the experimental population and analyze the associations with fat deposition traits.The expression level of ANO5 gene in different tissues was analyzed by qPCR.The results showed that sheep ANO5 gene was widely expressed in a variety of tissues in Hu sheep,and the highest expression of ANO5 gene was found in heart tissue compared with other tissues.Three genotypes of CC,CT and TT with the g.58010 C>T polymorphic locus were detected in the 10th intron of the sheep ANO5 gene.Descriptive statistics showed that the perirenal fat weight was the most different and had the highest degree of variability compared with other fat weights.Correlation analysis showed that fat deposition related traits were positively correlated with growth and feed efficiency traits,and the results of association analyses showed that the polymorphic locus was significantly associated with perirenal fat weight and its related traits in the Hu sheep.Among them,the perirenal fat weight of individuals with CC genotype was significantly lower than that of individuals with TT genotype.In conclusion,the g.58010 C>T mutation locus of the ANO5 gene can be used as a candidate molecular marker for perirenal fat deposition traits in Hu sheep.

Key words: Hu sheep, Anoctamin 5(ANO5), Polymorphism, Perirenal fat, Association analysis

HUANG Zhiqiang, WANG Weimin, ZHANG Deyin, ZHAO Yuan, ZHANG Yukun, XU Dan, YANG Xiaobin, MA Zongwu, HE Lijuan, CAI Youxin, LIU Xiaoqiang, ZHANG Xiaoxue. Analysis of ANO5 Gene Polymorphism and Its Association with Fat Deposition Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 207-214. doi: 10.7668/hbnxb.20194928.

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引物名称 Primer name	引物序列(5'—3') Primer sequences (5'—3')	产物大小/bp Size	最适温度/℃ TM
ANO5-PCR	F: GTGCTCCTTCATTATCCTT	710	46.3
	R: CAGAACAACTGCCTTCACA
ANO5-qPCR	F: AACCCCTTTTAGTTGTATGCTT	202	64.0
	R: ATCCTCCATGCCAAAAGGACA
UXT-qPCR	F: GCAAGTGGATTTGGGCTGTAAC	180	59.0
	R: ATGGAGTCCTTGGTGAGGCTGT

引物名称 Primer name	引物序列(5'—3') Primer sequences (5'—3')	产物大小/bp Size	最适温度/℃ TM
ANO5-PCR	F: GTGCTCCTTCATTATCCTT	710	46.3
	R: CAGAACAACTGCCTTCACA
ANO5-qPCR	F: AACCCCTTTTAGTTGTATGCTT	202	64.0
	R: ATCCTCCATGCCAAAAGGACA
UXT-qPCR	F: GCAAGTGGATTTGGGCTGTAAC	180	59.0
	R: ATGGAGTCCTTGGTGAGGCTGT

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
ANO5-Ra	GAAGGTGACCAAGTTCATGCTATCAAGTTTTAATCAGTTTGAATTTGTA
ANO5-Rg	GAAGGTCGGAGTCAACGGATTTCAAGTTTTAATCAGTTTGAATTTGTG
ANO5-F	GCCTTTTAATTTGATTAGGTAAGTTTC

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
ANO5-Ra	GAAGGTGACCAAGTTCATGCTATCAAGTTTTAATCAGTTTGAATTTGTA
ANO5-Rg	GAAGGTCGGAGTCAACGGATTTCAAGTTTTAATCAGTTTGAATTTGTG
ANO5-F	GCCTTTTAATTTGATTAGGTAAGTTTC

项目 Items	最大值 Max	最小值 Min	均值 Mean	标准差 s	变异系数/% CV
尾脂质量/kg The weight of tail fat	2.850	0.000	1.121	0.467	41.66
尾脂相对质量(体质量) The relative weight of tail fat (Body weight)	0.057	0.000	0.025	0.008	32.08
尾脂相对质量(胴体质量) The relative weight of tail fat (Carcass weight)	0.106	0.000	0.046	0.014	30.42
肾周脂肪质量/kg The weight of perirenal fat	6.850	0.005	0.562	0.439	78.11
肾周脂肪相对质量(体质量) The relative weight of perirenal fat (Body weight)	0.141	0.000	0.012	0.008	67.62
肾周脂肪相对质量(胴体质量) The relative weight of perirenal fat (Carcass weight)	0.254	0.001	0.022	0.014	65.27
肠系膜脂肪质量/kg The weight of mesenteric fat	3.060	0.005	0.877	0.488	55.67
肠系膜脂肪相对质量(体质量) The relative weight of mesenteric fat (Body weight)	0.051	0.001	0.019	0.008	44.20
肠系膜脂肪相对质量(胴体质量) The relative weight of mesenteric fat (Carcass weight)	0.092	0.001	0.035	0.015	42.47

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