Analysis of CEL Gene Polymorphisms and Their Association with Eye Muscle Area Traits in Hu Sheep

doi:10.7668/hbnxb.20195076

Abstract

Abstract:

The aim of this study was to investigate the correlation of single nucleotide polymorphisms of the carboxyl ester lipase gene (CEL) with eye muscle area in Hu sheep.The 1 049 Hu sheep with accurate phenotypic records and in good health were selected,their eye muscle area was determined after slaughter,and blood was collected for genomic DNA extraction.Amplification of target fragments and genotyping of CEL gene by PCR and KASPar SNP typing,and association analysis with eye muscle area of Hu sheep,RT-qPCR was used to detect the expression of CEL gene in 10 tissues of Hu sheep.The results showed that the Hu sheep CEL gene was expressed in different tissues and was highest in the duodenum.There was a synonymous mutation of g.4039718 C>T in this gene,which was moderately polymorphic.Trait association analysis showed that this polymorphic locus was significantly associated with eye muscle area in Hu sheep,with TT-type individuals having significantly higher eye muscle area than CC-type individuals.Descriptive statistics showed that the coefficient of variation for eye muscle area was 12.29%,which had high selection potential.Correlation analysis showed that eye muscle area was significantly and positively correlated with growth and slaughter traits such as body weight,body height,breast circumference,slaughter rate,carcass weight and live weight before slaughter.The results indicated that the g.4039718 C>T polymorphic locus could be used as a candidate molecular marker for genetic improvement of eye muscle area traits in Hu sheep.

Key words: Hu sheep, Carboxyl ester lipase (CEL), Eye muscle area, Association analysis

CAI Youxin, WANG Weimin, TIAN Huibin, ZHANG Deyin, ZHAO Yuan, ZHANG Yukun, XU Dan, YANG Xiaobin, MA Zongwu, HUANG Zhiqiang, LIU Xiaoqiang, HE Lijuan, HAN Kunchao, WU Weiwei, GAO Fei, WANG Lizhong, ZHANG Xiaoxue. Analysis of CEL Gene Polymorphisms and Their Association with Eye Muscle Area Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 231-238. doi: 10.7668/hbnxb.20195076.

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References 31

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引物功能 Primer functions	引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	产物长度/bp Product length	最佳退火温度/℃ Optimum annealing temperature
PCR扩增引物	CEL-F	CTCGGGACCTGGCCCATCCTCA	360	63
PCR amplification primer	CEL-R	ATTGAACCAGCTCCTCCGGTGA
RT-qPCR 引物	CEL-F	ATGTGCCAGCCATCAACAGCAA	190	61
RT-qPCR primer	CEL-R	ATATCGGTTTCCAGGTCCACCA
	UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	60
	UXT-R	ATGGAGTCCTTGGTGAGGCTGT

引物功能 Primer functions	引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	产物长度/bp Product length	最佳退火温度/℃ Optimum annealing temperature
PCR扩增引物	CEL-F	CTCGGGACCTGGCCCATCCTCA	360	63
PCR amplification primer	CEL-R	ATTGAACCAGCTCCTCCGGTGA
RT-qPCR 引物	CEL-F	ATGTGCCAGCCATCAACAGCAA	190	61
RT-qPCR primer	CEL-R	ATATCGGTTTCCAGGTCCACCA
	UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	60
	UXT-R	ATGGAGTCCTTGGTGAGGCTGT

项目 Items	天数 Days	最小值 Min	最大值 Max	平均值 Average	标准差 s	变异系数/% CV
体质量/kg	100	18.00	23.90	20.73	1.67	8.04
Body weight	140	28.50	34.40	31.55	1.73	5.48
	180	38.50	44.50	41.59	1.73	4.17
体高/cm	100	53.00	58.50	55.48	1.63	2.94
Body height	140	58.50	64.00	61.49	1.65	2.68
	180	62.00	67.00	64.66	1.57	2.43
体长/cm	100	55.50	61.34	58.36	1.66	2.85
Body length	140	63.00	68.50	65.73	1.70	2.59
	180	68.00	73.50	70.69	1.61	2.28
胸围/cm	100	59.50	65.00	62.25	1.77	2.84
Chest circumference	140	68.50	74.00	71.49	1.69	2.37
	180	78.00	83.00	80.50	1.72	2.13
初生质量/kg Birth weight	1	2.60	6.80	4.05	0.76	18.80
断奶质量/kg Weaning weight	56	11.80	17.70	14.49	1.64	11.31
饲料转化率/%	100~140	4.08	9.44	5.70	0.87	15.30
Feed conversion rate	140~180	3.41	9.11	6.96	1.08	15.57
胴体质量/kg Carcass weight	180	20.80	26.70	23.63	1.73	7.34
宰前活质量/kg Live weight before slaughter	180	41.20	47.10	43.83	1.71	3.91
屠宰率/% Dressing percentage	180	0.50	0.56	0.53	0.02	3.08
眼肌面积/cm² Eye muscle area	180	8.95	14.92	11.86	1.46	12.29

项目 Items	天数 Days	最小值 Min	最大值 Max	平均值 Average	标准差 s	变异系数/% CV
体质量/kg	100	18.00	23.90	20.73	1.67	8.04
Body weight	140	28.50	34.40	31.55	1.73	5.48
	180	38.50	44.50	41.59	1.73	4.17
体高/cm	100	53.00	58.50	55.48	1.63	2.94
Body height	140	58.50	64.00	61.49	1.65	2.68
	180	62.00	67.00	64.66	1.57	2.43
体长/cm	100	55.50	61.34	58.36	1.66	2.85
Body length	140	63.00	68.50	65.73	1.70	2.59
	180	68.00	73.50	70.69	1.61	2.28
胸围/cm	100	59.50	65.00	62.25	1.77	2.84
Chest circumference	140	68.50	74.00	71.49	1.69	2.37
	180	78.00	83.00	80.50	1.72	2.13
初生质量/kg Birth weight	1	2.60	6.80	4.05	0.76	18.80
断奶质量/kg Weaning weight	56	11.80	17.70	14.49	1.64	11.31
饲料转化率/%	100~140	4.08	9.44	5.70	0.87	15.30
Feed conversion rate	140~180	3.41	9.11	6.96	1.08	15.57
胴体质量/kg Carcass weight	180	20.80	26.70	23.63	1.73	7.34
宰前活质量/kg Live weight before slaughter	180	41.20	47.10	43.83	1.71	3.91
屠宰率/% Dressing percentage	180	0.50	0.56	0.53	0.02	3.08
眼肌面积/cm² Eye muscle area	180	8.95	14.92	11.86	1.46	12.29

位点 Loci	基因型 Genotype	个数 Number	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	Ne	Ho	He	PIC	HWE-χ²
g.4039718C>T	TT	64	0.06	T	0.28
	CT	424	0.43			1.68	0.6	0.4	0.32	0.59
	CC	511	0.51	C	0.72

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