Association Analysis of Verticillium Wilt-resistance in Upland Cotton Germplasm Population Based on SSR Markers

doi:10.7668/hbnxb.2018.06.021

Abstract

Abstract: The molecular markers related with Verticillium wilt-resistance were explored by association analysis, which could provide candidate markers for disease resistance for use in marker-assisted breeding (MAS). In 214 upland cotton materials, 237 SSR markers were assayed for polymorphism, and cluster analysis was performed by PowerMarker v3.25, population structure was analyzed by Structure 2.2, an association analysis between SSR markers and Verticillium wilt-resistance traits were performed using the Tassel 2.1 GLM(general linear model) program. The results showed that the 237 SSR markers included 695 alleles belong to 280 loci,with an average of 2.479 0 alleles per SSR marker(range, 2-6). Structure analysis showed that all the accessions could be divided into 2 subgroups. Of the SSR loci, 27 were significantly(P<0.01) associated with Verticilium wilt-resistance under GLM program in different years, among which 2 marker loci (BNL3442 and BNL1064) were found to be consistent associated with Verticillium wilt-resistance in 3 years, and the ratio of explanation on the phenotype variation of above markers was 12.10% and 8.02% respectively. These stable SSR markers in different years are likely to be closely linked to disease resistance genes and are expected to be used in the screening of cotton Verticillium wilt-resistance material and resistance gene mining.

Key words: Upland cotton, SSR, Verticillium wilt-resistance, Association analysis

CLC Number:

S435.62

ZHANG Sujun, TANG Liyuan, LI Xinghe, WANG Haitao, LIU Cunjing, ZHANG Xiangyun, ZHANG Jianhong. Association Analysis of Verticillium Wilt-resistance in Upland Cotton Germplasm Population Based on SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 152-159. doi: 10.7668/hbnxb.2018.06.021.

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