Analysis of Cytology and SSR for 6 New Potato Strains

doi:10.7668/hbnxb.20191721

Abstract

Abstract: In order to determine the difference of molecular and cellular genetics of six new potato strains (NNS-P 11, NNS-H 6,NNS-H 4, NNS-C 10, NNS-L 9 and NNS-Y 40), with MIN-021 as control material, conventional compression and SSR molecular labeling were used to compare and analyze the traits of the six new strains, include pollen fertility, PMCMⅠ chromosome pairing behavior, SSR fingerprint characteristics. This would provide the basis for further breeding, identification and registration of new potato varieties. The results showed that:There were some differences in pollen fertility among six new potato strains. The new strain NNS-Y 40 had the lowest pollen fertility (39.95%), the new strains NNS-P 11, NNS-H 4, NNS-C 10 had the highest pollen fertility (81.12%, 77.94%, 77.76%), the new strains NNS-H 6 and NNS-L 9 pollen fertility in the middle of the lowest and highest (67.27%, 69.71%). The chromosome pairing configuration of six new strains NNS-P 11, NNS-H 6, NNS-H 4, NNS-C 10, NNS-L 9 and NNS-Y 40 were 1.08Ⅰ+14.89Ⅱ+1.18Ⅲ+3.4 0Ⅳ, 2.35Ⅰ+11.96Ⅱ+3.59Ⅲ+2.74Ⅳ, 2.10Ⅰ+14.21Ⅱ+1.35Ⅲ+3.02Ⅳ, 3.31Ⅰ+13.98Ⅱ+2.99Ⅲ+1.94Ⅳ, 3.45Ⅰ+12.94Ⅱ+2.81Ⅲ+2.56Ⅳ and 8.23Ⅰ+6.92Ⅱ+6.07Ⅲ+1.93Ⅳ. The genomic DNA of six potato strains and control sample was amplified with 10 pairs SSR selected primers and 82 polymorphic bands were obtained, with the polymorphic ratio was 78.10%. The genetic distance (GD) of new potato strains ranged from 0.244 4-0.687 5. Base on 0.40 genetic distance, seven test materials were divided into three categories:the first category was new strains NNS-P 11, NNS-L 9, NNS-Y 40 and MIN-021, the second category was new strain NNS-H 6 and NNS-H 4, and the third category was new strain NNS-C 10. In addition, a SSR fingerprint was established to distinguish 7 test materials.

Key words: Potato, Pollen fertility, Chromosome configuration, SSR analysis

CLC Number:

S532.03

LI Liang, YU Zhuo, YU Xiaoxia, YANG Dongsheng, LU Qianqian, LI Jingwei, LI Jiaqi, LIU Yufei. Analysis of Cytology and SSR for 6 New Potato Strains[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 74-80. doi: 10.7668/hbnxb.20191721.

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