Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (5): 194-203. doi: 10.7668/hbnxb.20192773

Special Issue: Potato crops Plant protection Biotechnology

• Resources & Environment·Plant Protection • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of Resistant Gene StWRKY11 Induced by Sodium Silicate in Potato by Rhizoctonia solani

ZHANG Dongmei1,2, FENG Yayan1, XIU Zhijun1, YANG Chunfang1, DU Meie3, LI Dezhou4,5, ZHANG Xiaoyu1   

  1. 1 College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010020,China
    2 Plant Protection Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031,China
    3 Ecological Engineering Department,Ordos Vocational College of Ecological Environment, Ordos 017010,China
    4 College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010019, China
    5 Development Planning Office,Inner Mongolia Agricultural University,Hohhot 010018,China
  • Received:2022-06-16 Published:2022-10-28


In order to study the molecular mechanism of sodium silicate enhancing risistance of potato to Rhizoctonia solani,a gene StWRKY11 with high expression level in potato transcriptome induced by sodium silicate was cloned and its bioinformatics was analysed.Total RNA was extracted from potato,amplified by RT-PCR and cloned.Through bioinformatics related software,the structure prediction and prediction analysis were carried out.The results showed that StWRKY11 gene with a open reading frame of 1 005 bp was cloned from potato Atlantic,encoding 334 amino acids.The molecular formula of the expressed protein was C3013H5023N1005O1260S199,the molecular weight was 81.867 94 ku,the theoretical isoelectric point(pI)was 5.09,and the total number of atoms was 10 500.The expressed protein contained a typical WRKYGQK conserved domain,and the zinc finger structure was CX5CX23HXH,belonging to the second Ⅱ d subfamily.The secondary structural elements were α-helix,extended chain,β-folding and random coiling,among which the proportion of random curl was the highest,up to 61.68%.There were 29 phosphorylation sites in total,which might be located in the nucleus.There were cis-regulatory elements upstream of the promoter that might related to resistance stress response and cis-regulatory elements related to growth and development and hormone response.The gene was closely related to potato StWRKY5 gene,and the amino acid homology of the coding protein reached 95%.

Key words: Potato, StWRKY11, Gene cloning, Bioinformatics

Cite this article

ZHANG Dongmei, FENG Yayan, XIU Zhijun, YANG Chunfang, DU Meie, LI Dezhou, ZHANG Xiaoyu. Cloning and Bioinformatics Analysis of Resistant Gene StWRKY11 Induced by Sodium Silicate in Potato by Rhizoctonia solani[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 194-203. doi: 10.7668/hbnxb.20192773.

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