Cloning and Bioinformatics Analysis of Resistant Gene StWRKY11 Induced by Sodium Silicate in Potato by Rhizoctonia solani

doi:10.7668/hbnxb.20192773

Abstract

Abstract:

In order to study the molecular mechanism of sodium silicate enhancing risistance of potato to Rhizoctonia solani,a gene StWRKY11 with high expression level in potato transcriptome induced by sodium silicate was cloned and its bioinformatics was analysed.Total RNA was extracted from potato,amplified by RT-PCR and cloned.Through bioinformatics related software,the structure prediction and prediction analysis were carried out.The results showed that StWRKY11 gene with a open reading frame of 1 005 bp was cloned from potato Atlantic,encoding 334 amino acids.The molecular formula of the expressed protein was C₃₀₁₃H₅₀₂₃N₁₀₀₅O₁₂₆₀S₁₉₉,the molecular weight was 81.867 94 ku,the theoretical isoelectric point(pI)was 5.09,and the total number of atoms was 10 500.The expressed protein contained a typical WRKYGQK conserved domain,and the zinc finger structure was CX₅CX₂₃HXH,belonging to the second Ⅱ d subfamily.The secondary structural elements were α-helix,extended chain,β-folding and random coiling,among which the proportion of random curl was the highest,up to 61.68%.There were 29 phosphorylation sites in total,which might be located in the nucleus.There were cis-regulatory elements upstream of the promoter that might related to resistance stress response and cis-regulatory elements related to growth and development and hormone response.The gene was closely related to potato StWRKY5 gene,and the amino acid homology of the coding protein reached 95%.

Key words: Potato, StWRKY11, Gene cloning, Bioinformatics

ZHANG Dongmei, FENG Yayan, XIU Zhijun, YANG Chunfang, DU Meie, LI Dezhou, ZHANG Xiaoyu. Cloning and Bioinformatics Analysis of Resistant Gene StWRKY11 Induced by Sodium Silicate in Potato by Rhizoctonia solani[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 194-203. doi: 10.7668/hbnxb.20192773.

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Figures/Tables 11

References 44

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物种 Species	登录号 GenBank ID
辣椒 Capsicum annuum	AHD24521.1
茄子 Solanum melongena	AHI90951.1
枸杞 Lycium barbarum	ANW07460.1
天仙子 Hyoscyamus albus	BAN58138.1
番茄 Solanum lycopersic	NP001306910.1
烟草 Nicotiana tabacum	XP016497266.1
矮牵牛 Ipomoea nil	XP019174085.1
拟南芥 Arabidopsis thaliana	NP182248.1
马铃薯 Solanum tuberosum	XP006358112.1
	NP001274847.1

物种 Species	登录号 GenBank ID
辣椒 Capsicum annuum	AHD24521.1
茄子 Solanum melongena	AHI90951.1
枸杞 Lycium barbarum	ANW07460.1
天仙子 Hyoscyamus albus	BAN58138.1
番茄 Solanum lycopersic	NP001306910.1
烟草 Nicotiana tabacum	XP016497266.1
矮牵牛 Ipomoea nil	XP019174085.1
拟南芥 Arabidopsis thaliana	NP182248.1
马铃薯 Solanum tuberosum	XP006358112.1
	NP001274847.1

顺式元件 Cis-elements	序列 Sequence	元素的数量/个 Numbers of element	功能 Function
CAAT区 CAAT-box	CAAT	10	转录起始及增强元件
TATA区 TATA-box	TTATTT/TATAAAT	2	转录起始的关键调控位点
铜响应元件CURECORECR	GTAC	4	参与铜和氧的反应
MYB顺式元件 MYB2AT	TAACTG/AACGG	5	MYB 结合位点
DNA结合蛋白调控元件 DOFCOREZM	AAAG	12	DNA结合蛋白
转录激活元件 ARR1	GGATT/AGATT/CGATT/TGATT	17	转录因子参与基因表达的激活
CGCG区 CGCG-box	ACGCG/CCGCG/GCGCG	2	Ca²⁺钙调蛋白
W-区 W-box	TTGAC/TGAC	10	抗性胁迫响应调控元件
GATA区 GATA-box	GATA	9	光信号响应元件
光调控顺式元件 GT1CONSENSUS	CGTCA	11	光信号响应元件
ARE调控元件 ARE	AAACCA	1	缺氧诱导必需元件
叶肉特异基因表达调控元件CACTFTPPCA1	TACT/CACT	14	参与叶肉特异性表达
花粉调控元件 GTGANTG10	GTGA/AGAAA	12	花粉启动子元件
脱水顺式元件 CBFHV	ACCGAC/ATCGACGCCGAC/GTCGAC	4	脱水响应元件
赤霉素调控元件 PYRIMIDINEBOXOSRAMY1A	CCTTTT	1	赤霉素应答响应元件
茉莉酸调控元件 ASF1MOTIFCAMV	TGACG	2	茉莉酸信号响应调控元件
脱落酸响应元件 ABRE	ACGT/ACGTG	7	脱落酸响应元件

顺式元件 Cis-elements	序列 Sequence	元素的数量/个 Numbers of element	功能 Function
CAAT区 CAAT-box	CAAT	10	转录起始及增强元件
TATA区 TATA-box	TTATTT/TATAAAT	2	转录起始的关键调控位点
铜响应元件CURECORECR	GTAC	4	参与铜和氧的反应
MYB顺式元件 MYB2AT	TAACTG/AACGG	5	MYB 结合位点
DNA结合蛋白调控元件 DOFCOREZM	AAAG	12	DNA结合蛋白
转录激活元件 ARR1	GGATT/AGATT/CGATT/TGATT	17	转录因子参与基因表达的激活
CGCG区 CGCG-box	ACGCG/CCGCG/GCGCG	2	Ca²⁺钙调蛋白
W-区 W-box	TTGAC/TGAC	10	抗性胁迫响应调控元件
GATA区 GATA-box	GATA	9	光信号响应元件
光调控顺式元件 GT1CONSENSUS	CGTCA	11	光信号响应元件
ARE调控元件 ARE	AAACCA	1	缺氧诱导必需元件
叶肉特异基因表达调控元件CACTFTPPCA1	TACT/CACT	14	参与叶肉特异性表达
花粉调控元件 GTGANTG10	GTGA/AGAAA	12	花粉启动子元件
脱水顺式元件 CBFHV	ACCGAC/ATCGACGCCGAC/GTCGAC	4	脱水响应元件
赤霉素调控元件 PYRIMIDINEBOXOSRAMY1A	CCTTTT	1	赤霉素应答响应元件
茉莉酸调控元件 ASF1MOTIFCAMV	TGACG	2	茉莉酸信号响应调控元件
脱落酸响应元件 ABRE	ACGT/ACGTG	7	脱落酸响应元件

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