Acta Agriculturae Boreali-Sinica ›› 2025, Vol. 40 ›› Issue (3): 166-172. doi: 10.7668/hbnxb.20195409

Special Issue: Plant protection Biotechnology

• Resources & Environment·Plant Protection • Previous Articles     Next Articles

Cloning and Analysis of a FMRFamide-like Neuropeptide Gene from Bursaphelenchus doui

LIN Shifeng1,2, WANG Rengang1,2, , WANG Zili1,2, LI Li3, CHEN Yuenan3, WU Shasha3, LIU Guoqin4,   

  1. 1 Guizhou Academy of Tobacco Science,Guiyang 550081,China
    2 Key Laboratory of Molecular Genetics of CNTC,Guiyang 550081,China
    3 Guizhou Normal University,Guiyang 550081,China
    4 Guizhou University,Guiyang 550081,China
  • Received:2024-09-04 Published:2025-07-04

Abstract:

In order to explore the molecular mechanism of the interaction between Bursaphelenchus doui and host plants,an FMRFamide-like neuropeptide gene,named Bd-FLP-12,was cloned from B.doui by using RACE-PCR technique in a specific cDNA library of the anterior end of B.doui.Further,the nucleotide sequence and amino acid sequence of Bd-FLP-12 were analyzed using bioinformatics methods,the gene copy number was identified by Southern Blot,and the developmental expression pattern was investigated by semi-quantitative RT-PCR.The Bd-FLP-12 gene encoded a protein of 90 amino acids with a mature peptide sequence of FLP-12 and a signal peptide sequence but no transmembrane structure,indicating that its encoded protein was a secretory protein.Southern Blot indicated that the Bd-FLP-12 gene was a single copy gene in the B.doui genome,and the RT-PCR result showed that the transcription level of Bd-FLP-12 was lower in the egg stage than in the other stages.In summary,the full-length sequence of the Bd-FLP-12 gene was cloned for the first time in B.doui,and the structure,nature and expression of the gene were characterized,which could serve as a foundation for further study of the gene function.

Key words: Bursaphelenchus doui, FMRFamide-like neuropeptide, Bioinformatics, Southern Blot, Expression analysis

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Cite this article

LIN Shifeng, WANG Rengang, WANG Zili, LI Li, CHEN Yuenan, WU Shasha, LIU Guoqin. Cloning and Analysis of a FMRFamide-like Neuropeptide Gene from Bursaphelenchus doui[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 166-172. doi: 10.7668/hbnxb.20195409.

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