Acta Agriculturae Boreali-Sinica ›› 2025, Vol. 40 ›› Issue (3): 157-165. doi: 10.7668/hbnxb.20195636

Special Issue: Plant protection Biotechnology

• Resources & Environment·Plant Protection • Previous Articles     Next Articles

Establishment of Virus-Mediated Gene Silencing System in Cabbage

WANG Jiang1,2, LIU Yonggui2, HAN Rui2, ZHONG Xionghui2, , XIE Jianming1, KANG Jungen1,2,   

  1. 1 Colleage of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
    2 Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Beijing 100097,China
  • Received:2024-12-18 Published:2025-06-28

Abstract:

Virus-induced gene silencing(VIGS)is a post-transcriptional gene silencing technique widely used in plant gene function research.However,there are few reports on the establishment of a VIGS system for cabbage in China.The aim of this study was to establish a PCVA/PCVB-mediated gene silencing system using phytoene desaturase(PDS) as an effective visual indicator gene in cabbage.Cabbage,Chinese cabbage,and radish were used as plant materials.The PDS gene was silenced by constructing the PCVA-PDS vector.The PCVA/PCVB-PDS-GFP vector was then generated and transformed into Agrobacterium tumefaciens,which was used to infect cabbage and tobacco epidermal cells via injection and to perform vacuum infiltration in cabbage seedlings.Real-time Quantitative PCR was used to study the applicability of the VIGS system in cabbage,and the system was also applied to another representative Brassicaceae crops-Chinese cabbage and radish.The results showed that green fluorescence could be observed on the cell membranes of cabbage and tobacco leaf cells after infection with Agrobacterium tumefaciens transformed with PCVA/PCVB-PDS-GFP.After 14 d of vacuum infiltration in cabbage seedlings,photobleaching appeared on the new leaves,with the affected area gradually expanding.Real-time Quantitative PCR analysis indicated that the relative expression of PDS homologous genes in the experimental groups decreased 3.2-fold and 1.7-fold compared to the control group,respectively.After infecting Chinese cabbage and radish seedlings,whitening of the leaf veins and some leaves was observed,along with some leaf curling.In conclusion,the photobleaching observed in cabbage leaves after PDS gene silencing demonstrates that the VIGS system effectively replicates and spreads within cabbage plants.The whitening of leaves in Chinese cabbage and radish also indicates that the VIGS system can be applied to other Brassicaceae crops,thus expanding the application scope of this silencing system.The establishment of the cabbage VIGS system provides a theoretical foundation for functional gene studies in Brassicaceae crops.

Key words: Cabbage, Photobleaching, Gene silencing, Virus-mediation, PDS

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Cite this article

WANG Jiang, LIU Yonggui, HAN Rui, ZHONG Xionghui, XIE Jianming, KANG Jungen. Establishment of Virus-Mediated Gene Silencing System in Cabbage[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 157-165. doi: 10.7668/hbnxb.20195636.

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