A Rapid Identification Method and Application of a New Brassica napus CMS in Chinese Cabbage

doi:10.7668/hbnxb.20191301

Abstract

Abstract: At present, in the identification process of Pol CMS, Ogu CMS and New Brassica napus CMS, two pairs of primers must be used for molecular marker screening from orf138, orf222 and orf224. In order to develop a new type of molecular markers for rapid identification of New Brassica napus CMS, PCR amplification was carried out by orf222 that based on genomic DNA of CMS-AQ29 and CMS Hou36gao which respectively contained Pol CMS and New Brassica napus CMS.The results showed that the main difference genomic DNA between the two varieties was from 180 to 210 bp. A special primer of New Brassica napus CMS was designed and developed by T/G base-based mismatch on the end of the orf222 upstream primer terminal. It was named O-2. It could identify male sterile lines with New Brassica napus CMS immediately, 470 bp nucleotide sequence could be amplified,but nucleotide sequence could't be amplified in other resources without New Brassica napus CMS. The defect was solved that identification of male sterility must be pairs of primer. This method was resulted that is stable and reliable, the CMS-D571 and CMS-D574 are successfully identified by using this method that including New Brassica napus CMS from D571, D574, CMS-D571 and CMS-D574.

Key words: Chinese cabbage, New Brassica napus, Male sterile lines, Primer design, Molecular marker

CLC Number:

S634.03

LIU Xiaodong, LI Haishan, MENG Chuan, WANG Yuhai, WU Fang, MU Jingui, MA Junxian, WANG Mingqiu. A Rapid Identification Method and Application of a New Brassica napus CMS in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 26-31. doi: 10.7668/hbnxb.20191301.

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