Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (1): 42-49. doi: 10.7668/hbnxb.20192406

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Transcriptome Sequencing and Bioinformatics Analysis of Astragalus complanatus

WU Yue1,2, CHEN Yang2, WANG Xingzhe2, SHAN Feibiao2, ZHANG Yong2, SUN Hongju1   

  1. 1. School of Life Sciences,Inner Mongolia University,Hohhot 010020,China
    2. Bayannur Academy of Agricultural and Animal Sciences,Linhe 015000,China
  • Received:2021-10-04 Published:2022-02-28

Abstract:

In order to obtain transcriptome information and functional genes expression characteristics of Astragalus complanatus,the leaves of A.complanatus were used as material,the high-throughput transcriptome sequencing and bioinformatics analysis were performed using Illumina HiSeq platform.A total of 19 280 Unigenes were assembled with total sequence length of 23 472 470 bp,of which the GC content was 42.74%,indicating a high quality of sequencing and assembly.Blast analysis showed 12 541,10 120,9 412,8 953,7 494,5 052 Unigenes got annotation in Nr,Swiss-Prot,GO,KEGG,KOG and COG databases,respectively.The annotated Unigenes of A.complanatus were mainly homologuous to leguminous plants,in particular,highly matched to the subfamily Pteriformis plant Cicer arietinum,Medicago truncatula and Abrus precatorius with a respective percentage of 31.49%,14.50% and 11.65%.Annotated Unigenes were divided into 3 GO classifications,354 KEGG metabolic pathways and 25 KOG function categories.The most abundant categories in each database were metabolic process,general function prediction only and purine metabolism,respectively.These results showed that the leaf cells of Astragalus membranaceus had active metabolism and abundant gene expression in the seedling stage.Additionally,a lot of Unigenes were annotated to the infectious disease category in KEGG database,suggesting a potential medicinal value of the plant part of Astragalus membranaceus.A total of 5 849 simple sequence repeats(SSRs)were identified by MISA software,with an occurrence frequency of 30.34%.The SSRs in the genome of Astragalus membranaceus were abundant and diversified,containing single base to six base repeats,among which the mono-nucleotide SSRs had the largest number with a frequency of 40.56%.The main types of SSR motifs involved A/T,AG/CT and AAG/CTT.

Key words: Astragalus complanatus, Transcriptome, Bioinformatics, Unigene, Molecular marker

Cite this article

WU Yue, CHEN Yang, WANG Xingzhe, SHAN Feibiao, ZHANG Yong, SUN Hongju. Transcriptome Sequencing and Bioinformatics Analysis of Astragalus complanatus[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 42-49. doi: 10.7668/hbnxb.20192406.

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