Preliminary Study on the Mechanism of Laying Reflective Film to Promote the Coloring of Protected Grape

doi:10.7668/hbnxb.20193808

Abstract

Abstract:

To find out the effect of laying reflective film on fruit quality in facilities and the mechanism of promoting coloring,the changes of the appearance and anthocyanin content of Jingyan grape fruits were monitored with the film laid between rows,then transcriptome sequencing and differential expression gene analysis of the peels with different coloring were carried out using high-throughput sequencing and Illumina Novaseq 6000 sequencing platform.Finally,41 151 986—46 673 908 Clean reads were obtained from 12 samples,with Q30 was over 94% and Q20 was over 98%.Besides,the mapping ratio between Clean reads and grape reference genome was 92.69%—94.22%.The analysis results of differentially expressed genes (DEGS) showed that there were 3 768 DEGs in CK 0 d—CK 7 d,with 1 488 up-regulated and 2 280 down-regulated.And 5 129 in RF 0 d—RF 7 d after film mulching,with 2 048 up-regulated and 3 081 down-regulated.It showed that film mulching can increase the differential expression of genes.Compared with CK,934 DEGs were obtained after 7 days mulching,of which 441 genes were up-regulated and 493 were down-regulated.KEGG enrichment analysis showed that,a total of 179 DEGs were annotated on 64 signal pathways,which were mainly enriched in five metabolic pathways:metabolism,cellular process,environmental information processing,genetic information processing and organismal systems.Particularly,many genes vere differentially expressed in benzene propane biosynthesis,endoplasmic reticulum protein processing,plant pathogen interaction and plant hormone signaling pathway.Results showed some transcripts of PAL,F3'H and 3GT were up-expressed,and some auxin-related protein genes were down-regulated.For transcription factors,WRKY gene was mainly up-regulated,while bHLH,C2H2,NAC,MIKC-MADS,Dof were down-regulated.In conclusion,laying reflective film may improve the activity of phenylalanine ammonia-lyase(PAL)and the expression of related genes(F3'H,3GT),thus promoting anthocyanin synthesis.Meanwhile,transcriptional factors WRKY,bHLH,C2H2,MYB,NAC,MIKC-MADS and Dof may also play an important role in regulation.

Key words: Grape, Reflective film, Colour, Transcriptome, DEGs

NIU Zaozhu, ZHAO Yanzhuo, CHEN Zhan, XUAN Lifeng, NIU Shuaike, LI Yanan, YANG Lili. Preliminary Study on the Mechanism of Laying Reflective Film to Promote the Coloring of Protected Grape[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 203-210. doi: 10.7668/hbnxb.20193808.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20193808

http://www.hbnxb.net/EN/Y2023/V38/IS1/203

Figures/Tables 10

References 32

[1]	王立红. 设施果树栽培技术的应用初探[J]. 果树资源学报, 2020, 1(3):71-73.doi:10.16010/j.cnki.14-1127/s.2020.03.027.
	Wang L H. A preliminary study on the application of modern facility fruit tree cultivation technology[J]. Journal of Fruit Resources Guoshu, 2020, 1(3):71-73.
[2]	王世平, 李勃. 中国设施葡萄发展概况[J]. 落叶果树, 2019, 51(1):1-5.doi:10.13855/j.cnki.lygs.2019.01.001.
	Wang S P, Li B. General situation of protected grape development in China[J]. Deciduous Fruits, 2019, 51(1):1-5.
[3]	颜丽菊, 朱潇婷, 任海英, 朱建军, 马志方, 戚行江. 设施大棚铺设银色反光膜栽培杨梅试验研究[J]. 中国农学通报, 2014, 30(16):211-215.doi:10.3969/j.issn.1672-8246.2012.04.006.
	Yan L J, Zhu X T, Ren H Y, Zhu J J, Ma Z F, Qi X J. Experimental study on bayberry plants in the greenhouse with the soil covered with the silvery plastic film[J]. Chinese Agricultural Science Bulletin, 2014, 30(16):211-215. doi: 10.11924/j.issn.1000-6850.2013-3345
[4]	刘林, 许雪峰, 王忆, 李天忠, 韩振海. 不同反光膜对设施葡萄光合特性和叶片糖代谢的影响[J]. 西北植物学报, 2008, 28(3):559-563.doi:10.3321/j.issn:1000-4025.2008.03.022.
	Liu L, Xu X F, Wang Y, Li T Z, Han Z H. Effect of different reflecting films on photosynthetic characteristics and sucrose metabolism of grape leaves in greenhouse[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(3):559-563.
[5]	成果, 吴代东, 余欢, 冯启艳, 张劲, 谢林君, 白扬, 谢太理, 周咏梅, 周思泓. 反光膜和摘叶对毛葡萄果际微气候及其果实品质的影响[J]. 西南农业学报, 2020, 33(12):2912-2920.doi:10.16213/j.cnki.scjas.2020.12.033.
	Cheng G, Wu D D, Yu H, Feng Q Y, Zhang J, Xie L J, Bai Y, Xie T L, Zhou Y M, Zhou S H. Effect of application of reflecting film and leaf removal on microclimate around fruiting zone and fruit quality of Vitis quinquangularis Rehd.Grape[J]. Southwest China Journal of Agricultural Sciences, 2020, 33(12):2912-2920.
[6]	李志强, 刘洪亮, 田铃枝, 陈虎, 刘晓伟, 陈红梅, 吕军, 王磊. 反光膜对北疆滴灌葡萄生长、光合作用及品质的影响[J]. 浙江农业科学, 2019, 60(11):2034-2038,2041.doi:10.16178/j.issn.0528-9017.20191130.
	Li Z Q, Liu H L, Tian L Z, Chen H, Liu X W, Chen H M, Lü J, Wang L. Effects of reflective film on grape growth,photosynthesis and quality of drip irrigation grapes in northern Xinjiang[J]. Journal of Zhejiang Agricultural Sciences, 2019, 60(11):2034-2038,2041.
[7]	王立如, 房聪玲, 徐绍清, 高长达, 徐永江, 付涛, 王忠华, 吴月燕. 反光膜应用对大棚葡萄光合特性及果实品质的影响[J]. 江苏农业学报, 2014, 30(4):863-869.doi:10.3969/j.issn.1000-4440.2014.04.027.
	Wang L R, Fang C L, Xu S Q, Gao C D, Xu Y J, Fu T, Wang Z H, Wu Y Y. Effects of reflective films on photosynthetic characteristics and fruit quality of greenhouse grape[J]. Jiangsu Journal of Agricultural Sciences, 2014, 30(4):863-869.
[8]	王鑫, 于军, 鲍荆凯, 王艳, 闫敏, 吴翠云. 采前铺设反光膜对骏枣光合特性及果实品质的影响[J]. 塔里木大学学报, 2021, 33(1):85-91.doi:10.3969/j.issn.1009-0568.2021.01.011.
[9]	张卓, 王磊, 陈秋菊, 李俞涛, 高洁, 潘凤荣, 王世平, 张才喜. 铺设反光膜对日光温室甜樱桃果实品质的影响初报[J]. 上海交通大学学报(农业科学版), 2019, 37(4):24-28.doi:10.3969/J.ISSN.1671-9964.2019.04.005.
	Zhang Z, Wang L, Chen Q J, Li Y T, Gao J, Pan F R, Wang S P, Zhang C X. Effect of reflective film on fruit quality of sweet cherry(Prunus aviumcv.Tieton)in solar greenhouse[J]. Journal of Shanghai Jiao Tong University(Agricultural Science), 2019, 37(4):24-28.
[10]	陆泰良, 阳爱民, 李海炎, 梁瑞郑, 万保雄. 南方寡日照条件下铺设反光膜对避雨栽培早熟油桃品质的影响[J]. 西南农业学报, 2021, 34(6):1188-1194.doi:10.16213/j.cnki.scjas.2021.6.008.
	Lu T L, Yang A M, Li H Y, Liang R Z, Wan B X. Effect of laying reflective film on quality of early nectarine cultivated in rain-sheltered cultivation in South China with few sunshine[J]. Southwest China Journal of Agricultural Sciences, 2021, 34(6):1188-1194.
[11]	吴黎明, 蒋迎春, 王志静, 周民生, 许淼, 仝铸, 涂俊凡, 甘国平, 张楚雄. 地面覆盖反光膜对温州蜜柑果实着色及品质的影响研究[J]. 中国南方果树, 2009, 38(6):39-41.doi:10.3321/j.issn:0513-353X.2004.05.004.
	Wu L M, Jiang Y C, Wang Z J, Zhou M S, Xu M, Tong Z, Tu J F, Gan G P, Zhang C X. Effect of ground covering with reflective film on fruit coloring and quality of Satsuma mandarin[J]. South China Fruits, 2009, 38(6):39-41.
[12]	于咏, 孟江飞, 惠竹梅, 张振文. 反光膜和摘叶处理对红地球葡萄光合特性及果实品质的影响[J]. 北方园艺, 2017(11):33-40.doi:10.11937/bfyy.201711007.
	Yu Y, Meng J F, Xi Z M, Zhang Z W. Effects of reflective film and leaf removal on photosynthetic characteristics and quality of Red globe grape[J]. Northern Horticulture, 2017(11):33-40.
[13]	马瑞娟, 张斌斌, 张春华, 蔡志翔, 颜志梅. 采前除袋铺设反光膜对桃果实着色及相关基因表达的影响[J]. 园艺学报, 2015, 42(11):2123-2132.doi:10.16420/j.issn.0513-353x.2015-0290.
	Ma R J, Zhang B B, Zhang C H, Cai Z X, Yan Z M. Effect of bag removing with reflective film mulching before harvest on fruit coloration and expression of anthocyanin related genes in peach[J]. Acta Horticulturae Sinica, 2015, 42(11):2123-2132.
[14]	李云萍, 郭晋雅, 高峰. 紫心甘薯花青素积累与PAL活性的关系[J]. 西南大学学报(自然科学版), 2010, 32(2):68-72.doi:10.13718/j.cnki.xdzk.2010.02.018.
	Li Y P, Guo J Y, Gao F. The relationship between anthocyanidin accumulation and phenylalnine ammonialyase activity in purple-fleshed sweet potato(Ipomoea batatas(L.)lam.)[J]. Journal of Southwest University(Natural Science), 2010, 32(2):68-72.
[15]	Chen X J, Wang P J, Gu M Y, Hou B H, Zhang C R, Zheng Y C, Sun Y, Jin S, Ye N X. Identification of PAL genes related to anthocyanin synthesis in tea plants and its correlation with anthocyanin content[J]. Horticultural Plant Journal, 2022, 8(3):381-394.doi:10.1016/j.hpj.2021.12.005. URL
[16]	谢灵玲, 赵武玲, 沈黎明. 光照对大豆叶片苯丙氨酸裂解酶(PAL)基因表达及异黄酮合成的调节[J]. 植物学通报, 2000, 17(5):443-449.doi:10.3969/j.issn.1674-3466.2000.05.011.
	Xie L L, Zhao W L, Shen L M. Light regulation of the expression of PAL gene in soybean leaves and isoflavone synthesis[J]. Chinese Bulletin of Botany, 2000, 17(5):443-449.
[17]	刘卫红, 程水源. 光照及机械损伤对银杏叶苯丙氨酸解氨酶活性的影响[J]. 湖北农业科学, 2003, 42(3):73-75.doi:10.3969/j.issn.0439-8114.2003.03.032.
	Liu W H, Cheng S Y. Effects of the light and mechanical hurt on PAL activities in Ginkgo biloba leaf[J]. Hubei Agricultural Sciences, 2003, 42(3):73-75.
[18]	Sui X, Gao X, Ao M, Wang Q M, Yang D, Wang M, Fu Y, Wang L. cDNA cloning and characterization of UDP-glucose:anthocyanidin 3-O-glucosyltransferase in Freesia hybrida[J]. Reports, 2011, 30(7):1209-1218.doi:10.1007/s00299-011-1029-7.
[19]	Zhao Z C, Hu G B, Hu F C, Wang H C, Yang Z Y, Lai B. The UDP glucose:flavonoid-3-O-glucosyltransferase(UFGT)gene regulates anthocyanin biosynthesis in Litchi(Litchi chinesis Sonn.) during fruit coloration[J]. Molecular Biology Reports, 2012, 39(6):6409-6415.doi:10.1007/s11033-011-1303-3. URL
[20]	杜灵娟, 陈凯利, 刘雅莉. 葡萄风信子花青素3-O-葡糖基转移酶基因的克隆及其表达分析[J]. 草业科学, 2017, 34(11):2235-2244.doi: 10.11829/j.issn.1001-0629.2017-0049.
	Du L J, Chen K L, Liu Y L. Cloning and expression analysis of anthocyanidin 3-O-glucosyltransferase gene in Grape hyacinth[J]. Pratacultural Science, 2017, 34(11):2235-2244.
[21]	王应丽, 黄文俊, 王瑛. 箭叶淫羊藿EsUF3GT基因的克隆及表达分析[J]. 植物科学学报, 2014, 32(6):602-611.doi: 10.11913/PSJ.2095-0837.2014.60602.
	Wang Y L, Huang W J, Wang Y. Cloning and expression analysis of the EsUF3GT gene in Epimedium sagittatum(sieb.and zucc.)maxim[J]. Plant Science Journal, 2014, 32(6):602-611.
[22]	Kobayashi S, Goto-Yamamoto N, Hirochika H. Association of VvmybA1 gene expression with anthocyanin production in grape(Vitis vinifera)skin-color mutants[J]. Journal of the Japanese Society for Horticultural Science, 2005, 74(3):196-203.doi:10.2503/jjshs.74.196. URL
[23]	Walker A R, Lee E, Bogs J, McDavid D A J, Thomas M R, Robinson S P. White grapes arose through the mutation of two similar and adjacent regulatory genes[J]. The Plant Journal, 2007, 49(5):772-785.doi:10.1111/j.1365-313x.2006.02997.x. URL
[24]	Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, Richard T, Carde J P, Mérillon J M, Hamdi S. Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway[J]. Plant Physiology, 2006, 140(2):499-511.doi:10.1104/pp.105.067231. pmid: 16384897
[25]	Deluc L, Bogs J, Walker A R, Ferrier T, Decendit A, Merillon J M, Robinson S P, Barrieu F. The transcription factor VvMYB5b contributes to the regulation of anthocyanin and proanthocyanidin biosynthesis in developing grape berries[J]. Plant Physiology, 2008, 147(4):2041-2053.doi:10.1104/pp.108.118919. pmid: 18539781
[26]	Xie S, Qiao X L, Chen H W, Nan H, Zhang Z W. Coordinated regulation of grape berry flesh color by transcriptional activators and repressors[J]. Journal of Agricultural and Food Chemistry, 2019, 67(42):11815-11824.doi:10.1021/acs.jafc.9b05234. pmid: 31550160
[27]	许海峰. bHLH33与MYB抑制子参与苹果花青苷生物合成的分子机理[D]. 泰安: 山东农业大学, 2020.doi:10.27277/d.cnki.gsdnu.2020.000040.
	Xu H F. The molecular mechanism underlying anthocyanin biosynthesis in apple using bHLH33 and MYB repressors[D]. Taian: Shandong Agricultural University, 2020.
[28]	Hichri I, Heppel S C, Pillet J, Léon C, Czemmel S, Delrot S, Lauvergeat V, Bogs J. The basic helix-loop-helix transcription factor MYC1 is involved in the regulation of the flavonoid biosynthesis pathway in grapevine[J]. Molecular Plant, 2010, 3(3):509-523.doi:10.1093/mp/ssp118. pmid: 20118183
[29]	Martín-Pizarro C, Vallarino J G, Osorio S, Meco V, Urrutia M, Pillet J, Casañal A, Merchante C, Amaya I, Willmitzer L, Fernie A R, Giovannoni J J, Botella M A, Valpuesta V, Posé D. The NAC transcription factor FaRIF controls fruit ripening in strawberry[J]. The Plant Cell, 2021, 33(5):1574-1593.doi:10.1093/plcell/koab070. pmid: 33624824
[30]	Zhou H, Wang K L, Wang H L, Gu C, Dare A P, Espley R V, He H P, Allan A C, Han Y P. Molecular genetics of blood-fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors[J]. The Plant Journal, 2015, 82(1):105-121.doi:10.1111/tpj.12792. pmid: 25688923
[31]	毕思琦, 安建平, 王小非, 郝玉金, 芮麟, 李彤, 韩月彭, 由春香. 苹果乙烯响应因子MdERF3促进花青苷和原花青苷积累[J]. 园艺学报, 2019, 46(12):2277-2285.doi:10.16420/j.issn.0513-353x.2019-0122.
	Bi S Q, An J P, Wang X F, Hao Y J, Rui L, Li T, Han Y P, You C X. Ethylene response factor MdERF3 promotes anthocyanin and proanthocyanidin accumulation in apple[J]. Acta Horticulturae Sinica, 2019, 46(12):2277-2285. doi: 10.16420/j.issn.0513-353x.2019-0122
[32]	罗曼. C₂H₂型锌指蛋白DkZF6在柿原花青素生物合成中的功能解析[D]. 武汉: 华中农业大学, 2021.doi:10.27158/d.cnki.ghznu.2021.000243.
	Luo M. Functional characterization of C₂H₂ type zinc finger DkZF6 in persimmon proanthocyanidins biosynthesis[D]. Wuhan: Huazhong Agricultural University, 2021.
[8]	Wang X, Yu J, Bao J K, Wang Y, Yan M, Wu C Y. Effect of mulching reflective film on photosynthetic characteristics and fruit quality of Junzao jujube before harvest[J]. Journal of Tarim University, 2021, 33(1):85-91.

处理 Treatment	光照强度/lx Light intensity	净光合速率/ (μmol/(m²·s)) Pn
对照CK	5 635±645.20a	5.64±0.68a
反光膜Reflective films(RF)	8 345±1 029.69b	7.96±2.14b

处理 Treatment	光照强度/lx Light intensity	净光合速率/ (μmol/(m²·s)) Pn
对照CK	5 635±645.20a	5.64±0.68a
反光膜Reflective films(RF)	8 345±1 029.69b	7.96±2.14b

处理 Treatment	葡萄糖含量 Glucose content	果糖含量 Fructose content	酒石酸含量 Tartaric acid content	苹果酸含量 Malic acid content	柠檬酸含量 Citric acid content
对照CK	81.57±0.55a	96.24±0.53a	1.31±0.10a	2.61±0.18a	0.02a
反光膜Reflective films(RF)	83.16±0.42b	97.92±0.43b	1.25±0.15a	2.40±0.16a	0.02a

处理 Treatment	葡萄糖含量 Glucose content	果糖含量 Fructose content	酒石酸含量 Tartaric acid content	苹果酸含量 Malic acid content	柠檬酸含量 Citric acid content
对照CK	81.57±0.55a	96.24±0.53a	1.31±0.10a	2.61±0.18a	0.02a
反光膜Reflective films(RF)	83.16±0.42b	97.92±0.43b	1.25±0.15a	2.40±0.16a	0.02a

样品 Sample	原始序列 Raw reads	高质量序列 Clean reads	Q30/%	Q20/%	比对总数/% Total mapped reads	多比对读数/% Multiple mapped reads	唯一比对读数/% Uniquely mapped reads
CK 0 d 1	45 341 798	42 736 792	95.21	98.43	93.72	2.81	97.19
CK 0 d 2	48 254 250	45 442 318	94.40	98.04	92.69	3.08	96.92
CK 0 d 3	48 529 154	45 675 330	95.02	98.31	93.12	2.90	97.10
RF 0 d 1	46 623 352	43 904 078	94.80	98.24	94.16	2.82	97.18
RF 0 d 2	44 326 878	41 758 354	94.48	98.11	93.94	2.74	97.26
RF 0 d 3	43 667 922	41 151 986	94.78	98.25	93.76	2.70	97.30
CK 7 d 1	49 598 232	46 673 908	94.83	98.26	93.28	2.41	97.59
CK 7 d 2	48 166 082	45 329 912	94.88	98.28	92.85	2.44	97.56
CK 7 d 3	45 800 946	42 999 090	95.28	98.44	94.22	2.41	97.59
RF 7 d 1	49 097 782	46 201 014	94.82	98.27	93.70	2.43	97.57
RF 7 d 2	46 015 534	43 283 724	94.80	98.27	93.97	2.42	97.58
RF 7 d 3	46 802 176	44 036 412	94.76	98.24	93.58	2.38	97.62

Please choose a citation manager

Content to export