Comparative Transcriptome Analysis of Wheat Leaf in Response to Glufosinate-ammonium Stress

doi:10.7668/hbnxb.20193925

Abstract

Abstract:

In order to excavate the key genes in wheat which were resistant to glufosinate-ammonium stress and analyze family members chromosome distribution and expression pattern.Winter wheat Jinnong 6 was selected as the research object and different concentrations of glufosinate-ammonium was used in our study and observed symptoms of wheat after treatments.Total RNA was extracted from wheat leaves at 0,3 and 9 h after treatment.Transcriptomic sequencing technology was used to analyze and excavate the key genes in wheat.To confirm genes expression,protein structure of their family members and expression pattern.The results showed that TraesCS4A02G044000 was up-regulated induced by glufosinate-ammonium stress according to transcriptome analysis.Sequence analysis showed that TraesCS4A02G044000 was located in the 4th homologous gene A genome of wheat.The protein domain analysis showed that the protein encoded of TraesCS4A02G044000 had a signal peptide,a DUF568 and a b561.Twelve genes containing DUF568 and b561 domains were excavated from the genome family members,which were located in the 4th,5th and 7th homologous groups.The expression pattern analysis showed that TraesCS4D02G265000,TraesCS4B02G265100 and TraesCS4A02G044000 located in chromosomes A,B and D of the 4th homologous group which encoded cytochrome b561 were up-regulated obviously in wheat under glufosinate-ammonium stress. TraesCS4A02G044000 and its homologous genes encoding cytochrome b561 were significantly up-regulated under glufosinate-ammonium stress, indicated that b561 had a positive response to glufosinate-ammonium stress and participated in the metabolism of glufosinate-ammonium in wheat.

Key words: Wheat, Glufosinate-ammonium stress, Transcriptome analysis, Gene localization and expression pattern

YU Jinping, ZHANG Wei, LI Qi, BAI Penghua, CUI Xinyi, LIU Yixue. Comparative Transcriptome Analysis of Wheat Leaf in Response to Glufosinate-ammonium Stress[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(3): 184-190. doi: 10.7668/hbnxb.20193925.

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Figures/Tables 6

Fig.1 Phenotypes of wheat treated under glufosinate-ammonium at different concentrations

Fig.2 FPKM of TraesCS4A02G044000 after treatment of glufosinate-ammonium at different time Different letters indicate significant difference at the level of P<0.05. The same as Fig.5.

Fig.3 Structure of TraesCS4A02G044000 and encoding protein A. Sequence structure of TraesCS4A02G044000; B. Protein domain of TraesCS4A02G044000 encoded (protein domain consists of signal peptide, DUF568 and b561).

Tab.1 Family members of TraesCS4A02G044000

基因ID Gene ID	染色体位置 Chromosomal location	起始位置/bp Start position	终止位置/bp End position
TraesCS5B02G278100	5B	463 991 797	463 994 199
TraesCS4D02G265500	4D	435 626 360	435 629 320
TraesCS4B02G265500	4B	536 894 334	536 896 673
TraesCS7A02G255600	7A	243 646 927	243 650 472
TraesCS4B02G265100	4B	536 117 250	536 120 707
TraesCS7B02G152000	7B	202 160 045	202 164 168
TraesCS4A02G044400	4A	37 006 387	37 010 128
TraesCS5A02G278800	5A	488 022 430	488 024 910
TraesCS4A02G044000	4A	36 611 408	36 614 890
TraesCS4D02G265000	4D	435 206 769	435 210 339
TraesCS7D02G254000	7D	230 814 306	230 817 980
TraesCS5D02G285700	5D	386 150 155	386 152 541

Fig.4 Cluster analysis of TraesCS4A02G044000 family members

Fig.5 Gene expression pattern analysis of TraesCS4A02G044000 family members under glufosinate-ammonium stress

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