Abstract:

This study aims to provide theoretical guidance for the effective use of sister line materials,accelerate the promotion of the wheat Annong 1687 variety,and offer a reference for the genetic improvement of new wheat varieties.Wheat Annong 1687 and its sister lines and parents was used as materials.We combined phenotypic identification of field agronomic traits with genotypic identification of wheat materials using 55K microarrays.The data was analysed using SPSS and mapped it with the RIdeogram package.Significant differences in spike length were found between parents and some sister line combinations.Specifically,parents Annong 1106 and Xinong 822,and sister lines Annong 1687 and Xinong 822,showed very significant differences.The parental lines Annong 1106 and Xinong 822,as well as the sister lines Q6,8 and 105,133,exhibited a total of 909 differential SNP sites across 19 chromosomes.Notably,chromosome 5B contained 500 of these sites,which were concentrated in intervals of 63—87 Mb and 400—410 Mb.Cloning and sequencing of candidate genes within the intervals revealed that two genes encoding growth hormone-inducible proteins,TraesCS5B01G225000 and TraesCS5B01G058700,had missense mutations resulting in amino acid changes.Mutations in TraesCS5B01G225000 resulted in a codon change to the termination codon TGA and termination of amino acid synthesis.The bi-parental and sister line combinations had five and two missense mutations,respectively.It is hypothesized that these two genes may have an effect on the length of wheat spikes.

Key words: Wheat, Spike length, 55K chip, Sister line, Missense mutation