Comparative Analysis on the Transcriptome Profile of Wheat with Different Flour Qualities

doi:10.7668/hbnxb.20194398

Abstract

Abstract:

In order to elucidate the molecular mechanism of flour-quality regulation at the transcriptional level,wheat mutant varieties A94 and A261 were obtained by ⁶⁰Co-γ irradiation mutagenesis of Shimai 15,and the developing seeds at days 7,14,21 and 28 after flowering were subjected to transcriptome sequencing,and water absorption,dough development time,stable time,softening degree and farinogram index of the three varieties were measured.The results showed that farinogram parameters of two mutant varieties were better than those of Shimai 15.Twenty-four samples were sequenced,and after the data were filtered and annotated,a total of 26 714 genes were identified.With respect to Shimai 15,there were 1 042,258,301 and 366 differentially expressed genes(DEGs)at four time points in A94,respectively,and there were 2 178,248,117 and 959 DEGs in A261,respectively.KEGG enrichment showed that DEGs were mainly involved in pathways of starch and sucrose metabolism,protein processing in endoplasmic reticulum.GO enrichment showed that DEGs were mainly involved in glycogen biosynthesis processes,amyloplast and other biological processes.By analyzing the differential genes in these pathways and biological processes,it was hypothesized that the reasons for the improved flour quality in the mutant varieties include the up-regulation of genes related to sucrose and starch synthesis and metabolism in the early stages of filling,the degradation of misfolded proteins in the middle and late stages,and the differential expression of genes regulating protein and starch accumulation.

Key words: Wheat, Flour quality, Mutagenesis, Transcriptome, Starch, Protein

CLC Number:

LI Yaqing, ZHANG Nan, PENG Yifeng, ZHANG Shichang, HE Mingqi, SHI Zhanliang, LI Mengjun. Comparative Analysis on the Transcriptome Profile of Wheat with Different Flour Qualities[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 20-28. doi: 10.7668/hbnxb.20194398.

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References 33

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品种 Variety	吸水率/% Water absorption	形成时间/min Development time	稳定时间/min Stable time	弱化度/BU Softening degree	粉质质量指数 Farinogram quality number
石麦15 Shimai 15	60.6	2.2	1.2	195	29
A94	59.3	3.5	3.2	118	49
A261	59.7	2.9	3.0	109	45

品种 Variety	吸水率/% Water absorption	形成时间/min Development time	稳定时间/min Stable time	弱化度/BU Softening degree	粉质质量指数 Farinogram quality number
石麦15 Shimai 15	60.6	2.2	1.2	195	29
A94	59.3	3.5	3.2	118	49
A261	59.7	2.9	3.0	109	45

样品 Samples	清洁序列 Clean reads	清洁碱基数 Clean bases	GC含量/% GC content	Q30碱基率/% ≥Q30	配对序列(百分比) Mapped reads(percentage)
A261-14-1	35 481 965	10 603 342 024	52.77	91.71	57 193 498(80.60%)
A261-14-2	32 898 865	9 723 562 004	50.29	90.72	46 989 482(71.42%)
A261-21-1	33 946 628	10 165 735 340	52.93	90.29	53 956 147(79.47%)
A261-21-2	38 972 555	11 648 691 086	53.20	90.88	61 017 431(78.28%)
A261-28-1	44 257 733	13 231 568 456	55.23	92.37	77 802 729(87.90%)
A261-28-2	49 355 341	14 745 610 004	52.95	90.41	72 956 542(73.91%)
A261-7-1	36 185 229	10 823 893 150	55.72	94.05	67 154 530(92.79%)
A261-7-2	36 228 337	10 835 819 066	55.96	93.89	67 353 995(92.96%)
A94-14-1	35 418 707	10 574 388 830	52.88	90.18	54 682 919(77.19%)
A94-14-2	36 077 963	10 759 597 154	52.79	91.31	56 627 752(78.48%)
A94-21-1	35 582 247	10 626 652 626	52.77	90.65	53 968 799(75.84%)
A94-21-2	36 046 005	10 752 254 566	52.26	90.62	52 936 574(73.43%)
A94-28-1	43 508 983	12 968 858 488	53.44	90.44	65 095 024(74.81%)
A94-28-2	39 868 305	11 901 710 834	53.36	90.42	60 524 470(75.91%)
A94-7-1	39 638 786	11 852 116 112	55.84	93.40	73 767 842(93.05%)
A94-7-2	34 712 438	10 388 916 222	55.39	93.93	64 567 990(93.00%)
S15-14-1	34 535 415	10 321 437 346	52.76	91.35	52 834 434(76.49%)
S15-14-2	37 297 128	11 117 844 784	52.36	91.00	54 218 161(72.68%)
S15-21-1	34 796 650	10 374 910 010	51.97	90.33	47 807 168(68.70%)
S15-21-2	36 874 933	11 021 599 918	52.46	91.07	52 894 588(71.72%)
S15-28-1	37 430 294	11 164 262 956	55.05	90.80	59 261 949(79.16%)
S15-28-2	35 727 879	10 686 500 928	53.94	90.07	53 327 190(74.63%)
S15-7-1	37 006 995	11 067 505 840	55.19	92.75	66 579 079(89.95%)
S15-7-2	38 794 986	11 579 487 666	53.69	91.91	60 253 378(77.66%)

样品 Samples	清洁序列 Clean reads	清洁碱基数 Clean bases	GC含量/% GC content	Q30碱基率/% ≥Q30	配对序列(百分比) Mapped reads(percentage)
A261-14-1	35 481 965	10 603 342 024	52.77	91.71	57 193 498(80.60%)
A261-14-2	32 898 865	9 723 562 004	50.29	90.72	46 989 482(71.42%)
A261-21-1	33 946 628	10 165 735 340	52.93	90.29	53 956 147(79.47%)
A261-21-2	38 972 555	11 648 691 086	53.20	90.88	61 017 431(78.28%)
A261-28-1	44 257 733	13 231 568 456	55.23	92.37	77 802 729(87.90%)
A261-28-2	49 355 341	14 745 610 004	52.95	90.41	72 956 542(73.91%)
A261-7-1	36 185 229	10 823 893 150	55.72	94.05	67 154 530(92.79%)
A261-7-2	36 228 337	10 835 819 066	55.96	93.89	67 353 995(92.96%)
A94-14-1	35 418 707	10 574 388 830	52.88	90.18	54 682 919(77.19%)
A94-14-2	36 077 963	10 759 597 154	52.79	91.31	56 627 752(78.48%)
A94-21-1	35 582 247	10 626 652 626	52.77	90.65	53 968 799(75.84%)
A94-21-2	36 046 005	10 752 254 566	52.26	90.62	52 936 574(73.43%)
A94-28-1	43 508 983	12 968 858 488	53.44	90.44	65 095 024(74.81%)
A94-28-2	39 868 305	11 901 710 834	53.36	90.42	60 524 470(75.91%)
A94-7-1	39 638 786	11 852 116 112	55.84	93.40	73 767 842(93.05%)
A94-7-2	34 712 438	10 388 916 222	55.39	93.93	64 567 990(93.00%)
S15-14-1	34 535 415	10 321 437 346	52.76	91.35	52 834 434(76.49%)
S15-14-2	37 297 128	11 117 844 784	52.36	91.00	54 218 161(72.68%)
S15-21-1	34 796 650	10 374 910 010	51.97	90.33	47 807 168(68.70%)
S15-21-2	36 874 933	11 021 599 918	52.46	91.07	52 894 588(71.72%)
S15-28-1	37 430 294	11 164 262 956	55.05	90.80	59 261 949(79.16%)
S15-28-2	35 727 879	10 686 500 928	53.94	90.07	53 327 190(74.63%)
S15-7-1	37 006 995	11 067 505 840	55.19	92.75	66 579 079(89.95%)
S15-7-2	38 794 986	11 579 487 666	53.69	91.91	60 253 378(77.66%)

分组 Group	基因 Gene	基因个数 Gene count	上调/下调 Up/Down
A261-7 vs. S15-7	Cul1	4	上调
	EDEM	1	上调
	ERO1	3	上调
	GH47	1	上调
	Hsp20	11	上调
	Hsp70	5	混合
	Hsp90	3	上调
	Sec61	3	上调
	Skp1	1	下调
	PDIs	4	上调
	RMA3	2	上调
A261-14 vs. S15-14	HSP90	2	上调

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