Effector Protein Pt2567 Secried by Puccinia triticina Shows Avirulence in Wheat TcLr28

doi:10.7668/hbnxb.20195743

Abstract

Abstract:

The aim was to reveal the role of effector protein Pt2567 secreted by Puccinia triticina(Pt) in the pathogenesis and to lay a foundation for clarifying the interaction mechanism between effector protein and wheat.Tobacco transient expression technology,bacterial type Ⅲ secretion system,host induced gene silencing (HIGS) and other technologies were employed to carry out the subcellular localization,inhibition of BAX (mouse Bcl-2 family death promoting protein),effects on callose deposition and reactive oxygen species accumulation and preliminary function analysis of Pt2567.The results showed that the effector protein Pt2567 was subcellular located in the nucleus,and could inhibit BAX-induced programed cell death (PCD).Compared with control group,overexpression of effector protein Pt2567 in TcLr28 significantly enhanced the callose deposition and could increase the active oxygen accumulation at 24 h after the overexpression of the effector protein Pt2567 significantly.The silencing of effector protein Pt2567 on the TcLr28 inoculated with physiological race THTT significantly reduced the disease resistance of TcLr28,and increased the number of uredinium of Pt,and histological observation by confocal laser showed that silencing effector protein Pt2567 accelerated the development of wheat leaf rust.These results all suggest that effector protein Pt2567 plays a positive regulatory role in TcLr28,and is presumed to be a candidate avirulent gene for Lr28.

Key words: Puccinia triticina, Effector protein, Pt2567, Avirulent gene, Pathogenicity

CLC Number:

ZHANG Yingdan, ZHANG Yue, CHANG Jiaying, ZHANG Na, YANG Wenxiang. Effector Protein Pt2567 Secried by Puccinia triticina Shows Avirulence in Wheat TcLr28[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 177-183. doi: 10.7668/hbnxb.20195743.

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http://www.hbnxb.net/EN/Y2026/V41/I1/177

Figures/Tables 5

Fig.1 Subcellular localization of Pt2567 in N.benthamiana N.Nucleus;Bar=50 μm.

Fig.2 Pt2567 inhibits BAX-induced PCD in tobacco A.Schematic diagram of tobacco injection;B.Inside the red circle represents Pt2567 co-injected with BAX.

Fig.3 Overexpression of Pt2567 in TcLr28 promotes callose deposition and reactive oxygen species accumulation A.The callose of wheat leaves after injection of four bacterial solutions and aniline blue staining was observed(Bar=100 μm);B.Data statistics per square millimeter after four groups of treatments,different lowercase letters indicate significant differences(P<0.05),the same as Fig.4;C.Accumulation of reactive oxygen species after 24 h of inoculation with leaf rust fungus(Bar=50 μm);D.The area of reactive oxygen species per square millimeter after the three groups of treatments was counted.

Fig.4 Phenotypic observation and expression results of silencing Pt2567 A.Observation of viral inoculation phenotypes for 15 days;B.Observation of leaf phenotype at 10 days after inoculation with KHHT;C.Detecting the gene silencing efficiency of Pt2567.

Fig.5 Histological observation of silencing Pt2567 in TcLr28 under laser confocal microscopy AP.Attachment cell;IH.Infection hyphae;SV.Stomatal sac;HMC.Haustorial mother cells;H.Suction device;Bar=30 μm.

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