Effects of Bacillus velezensis and Streptomyces lydicus on Soil Microbes and Physicochemical Properties in Peach Orchards

doi:10.7668/hbnxb.20195768

Abstract

Abstract:

To evaluate the effects of microbial inoculants on soil microbial population and physicochemical properties,this study investigated the effects of the microbial inoculants,including Bacillus velezensis BPC6(B), Streptomyces lydicus (S), and their combined inoculants(BS) on the soil microbial abundance and physicochemical properties of field-grown and potted peach trees in Pinggu District,Beijing.The results showed that applying microbial inoculants(B,BS,and S) significantly increased the abundance of bacteria,fungi,and actinomycetes in field soils.Additionally,the microbial inoculants enhanced soil nutrient utilization efficiency by increasing the contents of soil organic matter,total carbon,total nitrogen,available nitrogen(alkaline-hydrolysis nitrogen),nitrate nitrogen,ammonium nitrogen,available potassium,total phosphorus,and available phosphorus,while no significant effect was observed on total potassium.Among them,during the peach harvest period,application of microbial inoculants B,BS,and S significantly increased soil available nitrogen by 11.89%,15.19%,and 35.27%,respectively,compared to the control.Available potassium increased significantly by 35.58%,22.92%,and 41.75%,respectively,while available phosphorus rose significantly by 37.97%,3.65%,and 62.60%,respectively.Correlation analysis further revealed that soil microbial abundance was significantly influenced by multiple soil environmental factors,particularly showing a strong positive correlation with available potassium content.The effect of improving soil fertility with different microbial inoculants(B,BS,and S) was as follows:microbial inoculant S was the most effective,and the combination application of B and S was better than that of individual application of inoculant B.The pot experiment further confirmed the important role of microbial inoculants in increasing soil available nitrogen levels.Compared to the control,application of microbial agents B,BS,and S significantly increased soil available nitrogen in potted peach Zhongpan 100 by 15.65%,16.67% and 13.45%,respectively;while microbial agents BS and S significantly increased available nitrogen in Zhongtao 14 by 13.65% and 18.13%,respectively.Overall,microbial inoculants effectively improved soil microbial abundance and fertility,providing scientific support and practical references for sustainable peach production.

Key words: Peach, Microbial inoculants, Bacillus velezensis, Streptomyces lydicus, Soil physicochemical properties, Soil microbial abundance

CLC Number:

HU Nana, ZUO Qiang, DONG Dan, YU Yang, TIAN Yu, JING Yanfu, WU Huiling, XIE Hua. Effects of Bacillus velezensis and Streptomyces lydicus on Soil Microbes and Physicochemical Properties in Peach Orchards[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 163-176. doi: 10.7668/hbnxb.20195768.

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References 63

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环境因子 Environmental factors	S1期 S1 stage	S3期 S3 stage	S4期 S4 stage
总碳 Total carbon	0.400 9^*	0.070 5	0.389 2^**
有机质 Organic matter	0.504 9^**	0.136 4	0.189 6
总氮 Total nitrogen	0.207 8	-0.050 5	0.256 5
铵态氮 Ammonium nitrogen	0.511 1^**	-0.005 9	-0.004 0
硝态氮 Nitrate nitrogen	0.590 5^**	-0.017 2	-0.101 2
碱解氮 Alkaline-hydrolyzable nitrogen	0.078 2	0.450 2^**	0.377 0^**
总钾 Total potassium	-0.146 2	-0.041 2	0.299 0^*
速效钾 Available potassium	0.445 3^*	0.463 3^**	0.780 0^**
总磷 Total phosphorus	0.073 8	0.068 9	0.415 8^**
有效磷 Available phosphorus	-0.249 4	0.190 9	0.160 6

环境因子 Environmental factors	S1期 S1 stage	S3期 S3 stage	S4期 S4 stage
总碳 Total carbon	0.400 9^*	0.070 5	0.389 2^**
有机质 Organic matter	0.504 9^**	0.136 4	0.189 6
总氮 Total nitrogen	0.207 8	-0.050 5	0.256 5
铵态氮 Ammonium nitrogen	0.511 1^**	-0.005 9	-0.004 0
硝态氮 Nitrate nitrogen	0.590 5^**	-0.017 2	-0.101 2
碱解氮 Alkaline-hydrolyzable nitrogen	0.078 2	0.450 2^**	0.377 0^**
总钾 Total potassium	-0.146 2	-0.041 2	0.299 0^*
速效钾 Available potassium	0.445 3^*	0.463 3^**	0.780 0^**
总磷 Total phosphorus	0.073 8	0.068 9	0.415 8^**
有效磷 Available phosphorus	-0.249 4	0.190 9	0.160 6

源 Source	因变量 Dependent variable	Ⅲ类平方和 Type Ⅲ sum of squares	自由度 Degree of freedom	均方 Mean square	F检验 F tests	显著性 Significance
修正模型	总碳	0.773a	11	0.070	79.787	0.000
Corrected model	有机质	353.529b	11	32.139	80.941	0.000
	总氮	0.007c	11	0.001	23.018	0.000
	铵态氮	5.862d	11	0.533	26.774	0.000
	硝态氮	376.136e	11	34.194	462.553	0.000
	碱解氮	2 480.990f	11	225.545	111.603	0.000
	总钾	0.122g	11	0.011	20.847	0.000
	速效钾	27 785.134h	11	2 525.921	163.010	0.000
	总磷	0.276i	11	0.025	184.495	0.000
	有效磷	13 901.219j	11	1 263.747	213.644	0.000
截距	总碳	32.967	1	32.967	37 438.565	0.000
Intercept	有机质	10 658.153	1	10 658.153	26 842.227	0.000
	总氮	0.593	1	0.593	21 344.400	0.000
	铵态氮	38.958	1	38.958	1 957.435	0.000
	硝态氮	4 333.150	1	4 333.150	58 615.489	0.000
	碱解氮	213 590.325	1	213 590.325	105 687.499	0.000
	总钾	158.256	1	158.256	298 284.314	0.000
	速效钾	2 273 521.150	1	2 273 521.150	146 721.328	0.000
	总磷	24.784	1	24.784	182 085.082	0.000
	有效磷	623 626.090	1	623 626.090	105 427.677	0.000
菌剂种类	总碳	0.407	3	0.136	154.081	0.000
Types of microbial	有机质	181.234	3	60.411	152.144	0.000
inoculants	总氮	0.004	3	0.001	48.667	0.000
	铵态氮	2.420	3	0.807	40.525	0.000
	硝态氮	68.964	3	22.988	310.965	0.000
	碱解氮	1 085.941	3	361.980	179.113	0.000
	总钾	0.002	3	0.001	1.159	0.346
	速效钾	7 801.240	3	2 600.413	167.817	0.000
	总磷	0.032	3	0.011	78.061	0.000
	有效磷	1 191.323	3	397.108	67.133	0.000
时期 Stages	总碳	0.193	2	0.096	109.461	0.000
	有机质	78.742	2	39.371	99.155	0.000
	总氮	0.001	2	0.001	24.300	0.000
	铵态氮	2.382	2	1.191	59.850	0.000
	硝态氮	239.521	2	119.760	1 620.024	0.000
	碱解氮	1 015.825	2	507.913	251.322	0.000
	总钾	0.112	2	0.056	105.188	0.000
	速效钾	98.727	2	49.363	3.186	0.059
	总磷	0.112	2	0.056	412.184	0.000
	有效磷	5 481.617	2	2 740.808	463.350	0.000
菌剂种类×时期	总碳	0.173	6	0.029	32.750	0.000
Types of microbial	有机质	93.553	6	15.592	39.268	0.000
inoculants×stages	总氮	0.002	6	0.000	9.767	0.000
	铵态氮	1.060	6	0.177	8.873	0.000
	硝态氮	67.651	6	11.275	152.522	0.000
	碱解氮	379.224	6	63.204	31.274	0.000
	总钾	0.008	6	0.001	2.578	0.045
	速效钾	19 885.168	6	3 314.195	213.881	0.000
	总磷	0.132	6	0.022	161.816	0.000
	有效磷	7 228.279	6	1 204.713	203.664	0.000
误差 Error	总碳	0.021	24	0.001
	有机质	9.530	24	0.397
	总氮	0.001	24	2.78E-05
	铵态氮	0.478	24	0.020
	硝态氮	1.774	24	0.074
	碱解氮	48.503	24	2.021
	总钾	0.013	24	0.001
	速效钾	371.892	24	15.496
	总磷	0.003	24	0.000
	有效磷	141.965	24	5.915
总计	总碳	33.761	36
Total	有机质	11 021.212	36
	总氮	0.601	36
	铵态氮	45.298	36
	硝态氮	4 711.060	36
	碱解氮	216 119.818	36
	总钾	158.391	36
	速效钾	2 301 678.180	36
	总磷	25.063	36
	有效磷	637 669.274	36
修正后总计	总碳	0.794	35
Corrected total	有机质	363.058	35
	总氮	0.008	35
	铵态氮	6.339	35
	硝态氮	377.910	35
	碱解氮	2 529.493	35
	总钾	0.134	35
	速效钾	28 157.027	35
	总磷	0.279	35
	有效磷	14 043.184	35

源 Source	因变量 Dependent variable	Ⅲ类平方和 Type Ⅲ sum of squares	自由度 Degree of freedom	均方 Mean square	F检验 F tests	显著性 Significance
修正模型	总碳	0.773a	11	0.070	79.787	0.000
Corrected model	有机质	353.529b	11	32.139	80.941	0.000
	总氮	0.007c	11	0.001	23.018	0.000
	铵态氮	5.862d	11	0.533	26.774	0.000
	硝态氮	376.136e	11	34.194	462.553	0.000
	碱解氮	2 480.990f	11	225.545	111.603	0.000
	总钾	0.122g	11	0.011	20.847	0.000
	速效钾	27 785.134h	11	2 525.921	163.010	0.000
	总磷	0.276i	11	0.025	184.495	0.000
	有效磷	13 901.219j	11	1 263.747	213.644	0.000
截距	总碳	32.967	1	32.967	37 438.565	0.000
Intercept	有机质	10 658.153	1	10 658.153	26 842.227	0.000
	总氮	0.593	1	0.593	21 344.400	0.000
	铵态氮	38.958	1	38.958	1 957.435	0.000
	硝态氮	4 333.150	1	4 333.150	58 615.489	0.000
	碱解氮	213 590.325	1	213 590.325	105 687.499	0.000
	总钾	158.256	1	158.256	298 284.314	0.000
	速效钾	2 273 521.150	1	2 273 521.150	146 721.328	0.000
	总磷	24.784	1	24.784	182 085.082	0.000
	有效磷	623 626.090	1	623 626.090	105 427.677	0.000
菌剂种类	总碳	0.407	3	0.136	154.081	0.000
Types of microbial	有机质	181.234	3	60.411	152.144	0.000
inoculants	总氮	0.004	3	0.001	48.667	0.000
	铵态氮	2.420	3	0.807	40.525	0.000
	硝态氮	68.964	3	22.988	310.965	0.000
	碱解氮	1 085.941	3	361.980	179.113	0.000
	总钾	0.002	3	0.001	1.159	0.346
	速效钾	7 801.240	3	2 600.413	167.817	0.000
	总磷	0.032	3	0.011	78.061	0.000
	有效磷	1 191.323	3	397.108	67.133	0.000
时期 Stages	总碳	0.193	2	0.096	109.461	0.000
	有机质	78.742	2	39.371	99.155	0.000
	总氮	0.001	2	0.001	24.300	0.000
	铵态氮	2.382	2	1.191	59.850	0.000
	硝态氮	239.521	2	119.760	1 620.024	0.000
	碱解氮	1 015.825	2	507.913	251.322	0.000
	总钾	0.112	2	0.056	105.188	0.000
	速效钾	98.727	2	49.363	3.186	0.059
	总磷	0.112	2	0.056	412.184	0.000
	有效磷	5 481.617	2	2 740.808	463.350	0.000
菌剂种类×时期	总碳	0.173	6	0.029	32.750	0.000
Types of microbial	有机质	93.553	6	15.592	39.268	0.000
inoculants×stages	总氮	0.002	6	0.000	9.767	0.000
	铵态氮	1.060	6	0.177	8.873	0.000
	硝态氮	67.651	6	11.275	152.522	0.000
	碱解氮	379.224	6	63.204	31.274	0.000
	总钾	0.008	6	0.001	2.578	0.045
	速效钾	19 885.168	6	3 314.195	213.881	0.000
	总磷	0.132	6	0.022	161.816	0.000
	有效磷	7 228.279	6	1 204.713	203.664	0.000
误差 Error	总碳	0.021	24	0.001
	有机质	9.530	24	0.397
	总氮	0.001	24	2.78E-05
	铵态氮	0.478	24	0.020
	硝态氮	1.774	24	0.074
	碱解氮	48.503	24	2.021
	总钾	0.013	24	0.001
	速效钾	371.892	24	15.496
	总磷	0.003	24	0.000
	有效磷	141.965	24	5.915
总计	总碳	33.761	36
Total	有机质	11 021.212	36
	总氮	0.601	36
	铵态氮	45.298	36
	硝态氮	4 711.060	36
	碱解氮	216 119.818	36
	总钾	158.391	36
	速效钾	2 301 678.180	36
	总磷	25.063	36
	有效磷	637 669.274	36
修正后总计	总碳	0.794	35
Corrected total	有机质	363.058	35
	总氮	0.008	35
	铵态氮	6.339	35
	硝态氮	377.910	35
	碱解氮	2 529.493	35
	总钾	0.134	35
	速效钾	28 157.027	35
	总磷	0.279	35
	有效磷	14 043.184	35

理化特性 Physicochemical properties	微生物菌剂 Microbial inoculant (I)	微生物菌剂 Microbial inoculant (J)		平均值差值 Mean difference (I-J)		标准差 Standard error		显著性 Significance		95% 置信区间 95% Confidence interval
理化特性 Physicochemical properties	微生物菌剂 Microbial inoculant (I)	微生物菌剂 Microbial inoculant (J)		平均值差值 Mean difference (I-J)		标准差 Standard error		显著性 Significance		下限 Lower bound		上限 Upper bound
总碳	CK		B		-0.07^*		0.014		0.000 0		-0.11		-0.03
Total carbon			BS		-0.18^*		0.014		0.000 0		-0.22		-0.14
			S		-0.28^*		0.014		0.000 0		-0.32		-0.24
	S		BS		0.21^*		0.014		0.000 0		0.17		0.25
			B		0.10^*		0.014		0.000 0		0.07		0.14
	BS		B		0.10^*		0.014		0.000 0		0.07		0.14
有机质	CK		B		-0.98^*		0.297		0.015 0		-1.80		-0.16
Organic matter			BS		-3.60^*		0.297		0.000 0		-4.42		-2.78
			S		-5.72^*		0.297		0.000 0		-6.54		-4.90
	S		BS		4.74^*		0.297		0.000 0		3.92		5.56
			B		2.12^*		0.297		0.000 0		1.30		2.94
	BS		B		2.62^*		0.297		0.000 0		1.80		3.44
总氮	CK		B		0.00		0.002		0.970 0		-0.01		0.01
Total nitrogen			BS		-0.02^*		0.002		0.000 0		-0.02		-0.01
			S		-0.03^*		0.002		0.000 0		-0.03		-0.02
	S		BS		0.02^*		0.002		0.000 0		0.02		0.03
			B		0.01^*		0.002		0.003 0		0.00		0.02
	BS		B		0.01^*		0.002		0.000 0		0.01		0.02
铵态氮	CK		B		0.18		0.067		0.058 0		0.00		0.36
Ammonium nitrogen			BS		-0.26^*		0.067		0.003 0		-0.45		-0.08
			S		-0.50^*		0.067		0.000 0		-0.69		-0.32
	S		BS		0.68^*		0.067		0.000 0		0.50		0.87
			B		0.24^*		0.067		0.007 0		0.06		0.43
	BS		B		0.44^*		0.067		0.000 0		0.26		0.62
硝态氮	CK		B		2.18^*		0.128		0.000 0		1.83		2.53
Nitrate nitrogen			BS		-0.90^*		0.128		0.000 0		-1.25		-0.54
			S		-1.46^*		0.128		0.000 0		-1.81		-1.10
	S		BS		3.63^*		0.128		0.000 0		3.28		3.99
			B		0.56^*		0.128		0.001 0		0.21		0.91
	BS		B		3.08^*		0.128		0.000 0		2.72		3.43
碱解氮	CK		B		-5.69^*		0.670		0.000 0		-7.53		-3.84
Alkaline-hydrolyzable			BS		-8.22^*		0.670		0.000 0		-10.07		-6.37
nitrogen			S		-15.29^*		0.670		0.000 0		-17.14		-13.45
	S		BS		9.61^*		0.670		0.000 0		7.76		11.46
			B		7.07^*		0.670		0.000 0		5.22		8.92
	BS		B		2.54^*		0.670		0.005 0		0.69		4.39
总钾	CK		B		-0.01		0.011		0.845 0		-0.04		0.02
Total potassium			BS		-0.01		0.011		0.956 0		-0.04		0.02
			S		0.01		0.011		0.794 0		-0.02		0.04
	S		BS		-0.02		0.011		0.492 0		-0.05		0.01
			B		-0.02		0.011		0.326 0		-0.05		0.01
	BS		B		0.00		0.011		0.990 0		-0.03		0.03
速效钾	CK		B		-21.61^*		1.856		0.000 0		-26.73		-16.49
Available potassium			BS		-30.51^*		1.856		0.000 0		-35.63		-25.39
			S		-39.72^*		1.856		0.000 0		-44.84		-34.60
	S		BS		18.11^*		1.856		0.000 0		12.99		23.22
			B		9.21^*		1.856		0.000 0		4.09		14.33
	BS		B		8.89^*		1.856		0.000 0		3.78		14.01
总磷	CK		B		-0.06^*		0.005		0.000 0		-0.07		-0.04
Total phosphorus			BS		-0.03^*		0.005		0.000 0		-0.05		-0.02
			S		-0.08^*		0.005		0.000 0		-0.10		-0.06
	S		BS		0.02^*		0.005		0.002 0		0.01		0.04
			B		0.05^*		0.005		0.000 0		0.03		0.06
	BS		B		-0.03^*		0.005		0.001 0		-0.04		-0.01
有效磷	CK		B		-3.16^*		1.147		0.050 0		-6.33		0.00
Available			BS		-3.16		1.147		0.051 0		-6.32		0.01
phosphorus			S		-15.05^*		1.147		0.000 0		-18.22		-11.89
	S		BS		11.89^*		1.147		0.000 0		8.73		15.05
			B		11.90^*		1.147		0.000 0		8.73		15.06
	BS		B		-0.01		1.147		1.000 0		-3.17		3.16

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