Cloning,Bioinformatics Analysis and Protein Induced Expression of 2-Cys Prx Gene in Feicheng Peach Fruits

doi:10.7668/hbnxb.2018.03.009

Abstract

Abstract: In order to study the regulation mechanism of 2-Cys Prx gene to redox signal molecules. 2-Cys Prx of Feicheng peach was studied by gene cloning and prokaryotic expression including PCR technique and RT-PCR technique. The experimental results showed that the full length of 2-Cys Prx gene was 1 424 bp induced 813 bp of coding sequence encoding a polypeptide of 270 amino acids,with predicted molecular weight of 29.7 ku. The predicted protein molecular formula of 2-Cys Prx was C₁₃₄₂H₂₁₁₀N₃₄₈O₄₀₃S₄ with a molecular weight of 29.696 ku,which contained 4 207 atoms. For 2-Cys Prx,the theoretical isoelectric point was about 6.23,and the total average hydrophobicity index was -0.126,which indicated that 2-Cys Prx protein was a hydrophilic protein. The two level prediction of 2-Cys Prx protein composed of 9 segment of fold and 4 segment of alpha helix and the irregular. Phylogenetic tree analysis showed that the 2-Cys Prx was most closely related to Prunus avium,with 98.15% amino acid homology rate. The high homology of amino acids of 2-Cys Prx in peach fruit and other plants,indicating that 2-Cys Prx was more conservative. These results would lay a foundation for researching the structure and function of 2-Cys Prx protein to explore the mechanism of 2-Cys Prx regulation of redox signal molecules in the further study.

Key words: Peach fruit, 2-Cys Prx, Gene cloning, Protein induced expression

CLC Number:

Q78
S662.03

ZHANG Yonglin, GAO Bin, ZHOU Jie, ZHU Shuhua. Cloning,Bioinformatics Analysis and Protein Induced Expression of 2-Cys Prx Gene in Feicheng Peach Fruits[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 50-55. doi: 10.7668/hbnxb.2018.03.009.

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