Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (1): 27-34. doi: 10.7668/hbnxb.20192488

Special Issue: Potato crops Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Cloning of DoIPTs Genes in Yam and Its Expression Analysis During Bulbil Germination

TANG Wenfang1, XU Shengsheng1, DUAN Yanbi2, LONG Wenhong1, YANG Rongping1, ZHANG Xuemei3, MENG Jingui1   

  1. 1. College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201,China
    2. Agricultural Teaching Experiment Center,Yunnan Agricultural University,Kunming 650201,China
    3. College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China
  • Received:2021-10-10 Published:2022-02-28


The aim of this research was to clone IPT(Isopentenyl transferase,IPT ) genes in yam,predict the structure and property of DoIPT proteins and identify the expression of DoIPTs during bulbil sprouting.DoIPTs were cloned by homologous cloning techniques.The structure and property of DoIPT proteins were predicted by bioinformatics analysis.Real-time PCR technique was applied to identify the expression of these genes.Three nucleic acid sequences of 1 398,1 044,1 349 bp were obtained from yam.The three sequences were named DoIPT1,DoIPT5a and DoIPT5b,and the registration numbers of these genes were MW353160,MW353016 and MW353017,respectively.The reading frame lengths were 1 137,861,1 011 bp respectively,and the encoded amino acid lengths were 378,286,336 aa,respectively.Bioinformatics analysis showed that DoIPTs were unstable,hydrophilic and external family proteins.There were two signal peptides in DoIPT5b,and no signal peptide was found in the other two proteins.The secondary structures in the three proteins were consistent with the prediction models of the tertiary structures of three genes.The encoded regions of DoIPTs had high similarity with the isopentenyl transferase protein of other plants.Coding areas of DoIPTs showed that the N-terminal regions of DoIPTs contained the P-loop NTPase structural domain GXXGXGKS(T),which was able to catalyse cytokinin synthesis.The results of Real-time PCR technique showed that the expression of DoIPT5a in sprouting bulbils was highest under the condition of water shortage at 22 ℃;and the expression of DoIPT1 and DoIPT5b genes was the highest in a humid environment;the expression of DOIPTs genes raised with paclobutrazol treatment.These results laid a foundation for further study on the function of DoIPTs in yam.

Key words: Yam, Gene cloning, DoIPT gene, Bulbil, Paclobutrazol(PP333), Relative quantitative expression

Cite this article

TANG Wenfang, XU Shengsheng, DUAN Yanbi, LONG Wenhong, YANG Rongping, ZHANG Xuemei, MENG Jingui. Cloning of DoIPTs Genes in Yam and Its Expression Analysis During Bulbil Germination[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 27-34. doi: 10.7668/hbnxb.20192488.

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