The objective of the study was to screen the candidate genes and SNP markers for the total number born(TNB)and number born alive(NBA)in pigs,so as to prepare for the next step of improving the reproduction traits,and breeding new high-proliferation of Canadian line of large white pigs. Illumina porcine 50K chip was used to do a genome-wide SNP scan on 186 Canadian line of large white sows in one pig farm,followed by quality control,beagle fill and SNP genotyping. Combined with the phenotypic traits and population structure analysis results of these experimental pigs,genome-wide association study(GWAS)was carried out. The results of population structure analysis showed that there was no population stratification in the pig samples used in the experiment. A total of 36 867 effective SNP markers were obtained on 18 pairs(36)of autosomes for GWAS analysis of experimental pigs. The results of GWAS showed that NBA in all parities of these Canadian large white pigs was significantly associated with two SNPs:seq-rs323899658 and seq-rs329781338,respectively. The SNP of seq-rs329781338 was annotated with three genes:SSBP1,WEE2 and KIAA1147,respectively. NBA was significantly associated with 7 SNPs,namely:seq-rs81238474,seq-rs321377412,seq-rs340736313,seq-rs80782154,seq-rs81244816,seq-rs81467772 and seq-rs329781338. Five out of seven SNPs had gene annotation,and a total of 22 genes were annotated:EphA1, TAS2R60, FAM131B, CLCN1, CASP2, TMEM139, TRBV21OR9-2, PRSS2, TRBV19, TRBV24-1, U6, SSBP1, WEE2, KIAA1147, NKX2-8, ALKBH3, HSD17B12, U6, HOMER2, WHAMM, FSD2 and SCARNA15. The results of GO function and KEGG pathway analysis showed that the most significant biological process of GO function of these genes was the regulation of multi biological process,the molecular function was non transmembrane/transmembrane protein tyrosine kinase activity,and the KEGG pathway was mismatch repair.Combined with GWAS and gene function annotation results,WEE2 and EphA1 genes could be used as key candidate genes to increase the TNB and NBA of Canadian large white pigs,and provide some reference for the precise cultivation of high-yield pigs in the future.