Acta Agriculturae Boreali-Sinica ›› 2024, Vol. 39 ›› Issue (5): 52-62. doi: 10.7668/hbnxb.20194755

Special Issue: Sweet pepper Biotechnology Vegetables

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Analysis of MADS-box Transcription Factor Family Based on Transcriptome Sequencing in Peppers

DING Chuqi, WU Peng, GUO Qianqian, WANG Li, GUO Zhanxin, FENG Bailong   

  1. College of Life Sciences and Agriculture and Forestry,Qiqihar University,Heilongjiang Provincial Key Laboratory of Resistance Genetic Engineering and Coldland Biodiversity Conservation,Qiqihar 161006,China
  • Received:2024-05-12 Published:2024-10-28

Abstract:

MADS-box transcription factors are widely found in plants and play important roles in plant growth and development and secondary metabolism.To investigate the expression of MADS-box transcription factor family in different accumulation periods of capsaicin.MADS-box transcription factor family was identified by using transcriptome data from different accumulation periods of capsaicin,and their functions were preliminarily analyzed.Subcellular localization,conserved motifs,phylogenetic tree and chromosomal localization of members of the MADS-box transcription factor family of chili peppers were carried out.The results showed that a total of 95 MADS-box transcription factors were identified in the Capsicum annuum L.transcriptome data;containing 105-395 amino acids;with molecular weight ranging from 11.55-44.46 ku;with theoretical isoelectric points ranging from 5.16-10.01;mainly expressed in the nucleus,and all of them contained MADS conserved structural domains,and phylogenetic analysis showed that MADS proteins could be divided into eight subfamilies.There were 73 CaMADS family members localized to 12 chromosomes.There were 26 differentially expressed MADS-box genes,six of which were up-regulated during C1 vs C2 and down-regulated during C2 vs C3.Based on KEGG enrichment and protein interactions,it was predicted that CaMADS13 might be involved in lignin synthesis in chili peppers.CaMADS24 might be involved in the synthesis of capsaicin and lignin synthesis precursor,coumaroyl-coenzyme A.Bioinformatics analysis was used to identify the MADS-box family of transcription factors in chili peppers,which provides data reference and theoretical basis for in-depth study of the molecular mechanism of action in the secondary metabolism of capsaicin.

Key words: Peppers, MADS-box, Transcription factor, Secondary metabolism

Cite this article

DING Chuqi, WU Peng, GUO Qianqian, WANG Li, GUO Zhanxin, FENG Bailong. Analysis of MADS-box Transcription Factor Family Based on Transcriptome Sequencing in Peppers[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 52-62. doi: 10.7668/hbnxb.20194755.

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