Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (5): 9-15. doi: 10.7668/hbnxb.20193082

Special Issue: Biotechnology Hot Article

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Cloning of SnRK2.4 Gene and It's Response to Abiotic Stress in Kentucky Bluegrass

JIN Yifeng1,2, GAO Yansong1, WANG Qi1, WANG Mengmeng1, ZHAO Di1, XIONG Yi1, CHEN Yang1,2,   

  1. 1 College of Life Science and Agro-Forestry,Qiqihar Univesity,Qiqihar 161006,China
    2 Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar 161006,China
  • Received:2022-07-17 Published:2022-10-28
  • Contact: CHEN Yang


Protein kinases are important factors in plant defense system,protein kinase SnRK2 is a serine/threonine protein kinase,can play an important role in the plant stress signal transduction pathway through phosphorylation.We analyzed the expression pattern of SnRK2.4 under abiotic stresses,aiming to reveal its role in the regulation of adversity.The SnRK2.4 gene of high-quality cold-season turfgrass Poa pratensis L. was cloned using RT-PCR,the SnRK2.4 gene contained an ORF of 1 092 bp encoding a 363-amino acid protein,and its molecular characteristics were analyzed by bioinformatics.In addition,the expression pattern of this gene in different tissue parts under different abiotic stresses was observed using qPCR.The results showed that the Poa pratensis L.SnRK2.4 gene belonged to the SRK/SAPK superfamily,contained typical STKc_SnRK2 domain,tyrosine kinase phosphorylation site,casein kinase Ⅱ phosphorylation site and serine/threonine-protein kinase activity site,which had the highest homology with Brachypodium distachyon.The qPCR results demonstrated that the Poa pratensis L.SnRK2.4 gene was tissue-specific,highly expressed in panicles,and had no significant differential expression in roots,stems and leaves.Moreover,the Poa pratensis L.SnRK2.4 gene could respond positively to abiotic stresses such as drought,salt,low nitrogen,low phosphorus,ABA and BR.

Key words: Kentucky bluegrass, Protein kinase, SnRK2.4, Gene cloning, Abiotic stress

Cite this article

JIN Yifeng, GAO Yansong, WANG Qi, WANG Mengmeng, ZHAO Di, XIONG Yi, CHEN Yang. Cloning of SnRK2.4 Gene and It's Response to Abiotic Stress in Kentucky Bluegrass[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 9-15. doi: 10.7668/hbnxb.20193082.

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