Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (1): 1-8. doi: 10.7668/hbnxb.20192627

Special Issue: Wheat Biotechnology Hot Article

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Cloning and Expression Analysis of Calmodulin-like Protein TaCML8-A Gene in Wheat

JIA Weizhe1,2, JIAO Bo2, WANG Jiao2, CHEN Wenye2, YANG Fan2, LIU Yongwei2, DONG Fushuang2, ZHAO Liqun1, ZHOU Shuo2   

  1. 1. College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China
    2. Institute of Biotechnology and Food Science,Hebei Academy of Agriculture and Forestry Sciences, Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,China
  • Received:2021-11-15 Published:2022-02-28


In order to explore stress resistance genes in wheat and study the molecular mechanism of calmodulin-like genes in plant stress resistance,a calmodulin-like gene TaCML8-A was cloned from wheat by electronic cloning combined with RT-PCR.Bioinformatics analysis showed that the open reading frame length of the gene was 519 bp,encoding 172 amino acid sequences,including PTZ00184 and FRQ1 superfamily,four EF-hand domains,including four calcium binding sites.The molecular weight of the encoded protein was 18.31 ku and the isoelectric point was 4.54.It belonged to acidic protein.Subcellular localization showed that TaCML8-A protein was distributed in the nucleus and cell membrane.Nucleotide sequence alignment showed that TaCML8-A had the closest genetic relationship with rice OsCML14,with a similarity of 79.17%.qRT-PCR results showed that the expression of TaCML8-A gene in shoot and root of wheat increased under salt,osmotic and cold stress,indicating that plants might respond to thoese stresses by increasing the expression of TaCML8-A.During heat shock,TaCML8-A gene was induced and inhibited in root and shoot,respectively,so as to play different functions.The calmodulin-like gene TaCML8-A was successfully cloned from wheat and analyzed for expression.It is preliminarily speculated that the gene might be involved in regulating abiotic stress of plants,so as to provide basic theoretical support for the follow-up study of its biological function.

Key words: Triticum aestivum, Calmodulin-like protein, EF-hand domain, Gene cloning, Gene expression

Cite this article

JIA Weizhe, JIAO Bo, WANG Jiao, CHEN Wenye, YANG Fan, LIU Yongwei, DONG Fushuang, ZHAO Liqun, ZHOU Shuo. Cloning and Expression Analysis of Calmodulin-like Protein TaCML8-A Gene in Wheat[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 1-8. doi: 10.7668/hbnxb.20192627.

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