Effect of Different NDF Levels of Starter on Growth Axis and Duodenum-Related Gene Expression in Lambs

doi:10.7668/hbnxb.20194254

Abstract

Abstract:

The aim of this trial was to investigate the effect of different levels of NDF on the expression of genes related to growth axis and duodenal growth in lambs,in order to provide a reference for the appropriate NDF level for lamb starter.Thirty-six male Hu lambs were selected for the trial and randomly divided into 15% and 25% NDF level groups.At 56 days of age,six lambs from each group were selected for slaughter,weighed for tissue and organ,and the expression of relevant genes was measured.The results showed that there was no significant effect(P>0.05)of NDF levels in the starter diet on tissue and organ mass and index;in the 15% group of lambs,hypothalamic growth hormone releasing hormone(GHRH),hepatic insulin-like growth factor-1(IGF-1),duodenal IGF-1 and IGF-1 receptor(IGF-1R)expressions were significantly higher in the 25% group(P<0.05);the expression of pituitary growth hormone(GH)and growth hormone receptor(GHR) in the duodenum were significantly higher in the 25% group than in the 15% group(P<0.01).The average daily gain of lambs was strongly negatively correlated with hypothalamic GHRH expression(P<0.01),significantly negatively correlated with hepatic GHR and duodenal IGF-1 expression(P<0.05),and significantly positively correlated with pituitary GH and duodenal GHR expression(P<0.05);pituitary GH was significantly or highly significantly negatively correlated with hepatic GHR,IGF-1,duodenal IGF-1 and IGF-1R expression(P<0.05 or P<0.01),and strongly significantly positively correlated with duodenal GHR expression(P<0.01);hepatic GHR was strongly significantly positively correlated with hepatic IGF-1,duodenal IGF-1 expression(P<0.01),and strongly significantly negatively correlated with duodenal GHR expression(P<0.01).In conclusion,the expression of genes related to GH-GHR levels in the growth axis of lambs is enhanced by feeding 25% NDF level open feed compared to the 15% level group.

Key words: Lamb, Starter feed, Growth axis, Duodenum, Gene expression

HAN Xiaoxia, ZHAO Haibi, LIU Guohua, LÜ Feng, PANG Xin, YANG Fan, WANG Xiaojuan. Effect of Different NDF Levels of Starter on Growth Axis and Duodenum-Related Gene Expression in Lambs[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(6): 211-217. doi: 10.7668/hbnxb.20194254.

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Figures/Tables 5

References 42

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基因 Genes	引物序列(5'—3') Primer sequence(5'—3')	编号序列 Accession number
生长激素释放激素基因 GHRH	F:ATGCTGCTCTGGGTGTTCTT R:CCTTCCGGTAGCTGTTAGTG	XM_005688533.3
生长激素基因 GH	F:GATACTCCATCCAAAACACCC R:AAGACTCTGCTGAGGAACTGC	NM_001285586.1
生长激素受体基因 GHR	F:GTGAGAACCCGACAAGGAA R:CACTGTTAGCCCAAGTATTCCA	NM_001285648.1
胰岛素样生长因子1基因 IGF-1	F:AAGATGCCAGTCACATCCTCC R:ATAAAAGCCCCTGTCTCCAC	NM_001285697.1
胰岛素生长因子受体基因 IGF-1R	F:AGCAAAGGCGACATAAACAC R:GGCTTCCTTGTAGTAGACGGT	XM_018065947.1
β-肌动蛋白基因 β-actin	F:GCTCTTCCAGCCGTCCTT R:TGAAGGTGGTCTCGTGAATGC	XM_001009784.1

基因 Genes	引物序列(5'—3') Primer sequence(5'—3')	编号序列 Accession number
生长激素释放激素基因 GHRH	F:ATGCTGCTCTGGGTGTTCTT R:CCTTCCGGTAGCTGTTAGTG	XM_005688533.3
生长激素基因 GH	F:GATACTCCATCCAAAACACCC R:AAGACTCTGCTGAGGAACTGC	NM_001285586.1
生长激素受体基因 GHR	F:GTGAGAACCCGACAAGGAA R:CACTGTTAGCCCAAGTATTCCA	NM_001285648.1
胰岛素样生长因子1基因 IGF-1	F:AAGATGCCAGTCACATCCTCC R:ATAAAAGCCCCTGTCTCCAC	NM_001285697.1
胰岛素生长因子受体基因 IGF-1R	F:AGCAAAGGCGACATAAACAC R:GGCTTCCTTGTAGTAGACGGT	XM_018065947.1
β-肌动蛋白基因 β-actin	F:GCTCTTCCAGCCGTCCTT R:TGAAGGTGGTCTCGTGAATGC	XM_001009784.1

项目 Items	15%组 15% group	25%组 25% group
宰前活质量/kg Slaughter weight	8.00±1.05a	8.09±1.60a
组织器官相对宰前活质量/% Relative slaughter weight of tissues and organs
头Head	8.13±0.61a	8.23±1.31a
心脏Heart	0.54±0.07a	0.56±0.10a
肝脏Liver	2.18±0.11a	2.25±0.33a
脾脏Spleen	0.14±0.02a	0.15±0.04a
肺脏Lungs	1.60±0.22a	1.49±0.32a
肾脏Kidneys	0.46±0.04a	0.50±0.09a

项目 Items	15%组 15% group	25%组 25% group
宰前活质量/kg Slaughter weight	8.00±1.05a	8.09±1.60a
组织器官相对宰前活质量/% Relative slaughter weight of tissues and organs
头Head	8.13±0.61a	8.23±1.31a
心脏Heart	0.54±0.07a	0.56±0.10a
肝脏Liver	2.18±0.11a	2.25±0.33a
脾脏Spleen	0.14±0.02a	0.15±0.04a
肺脏Lungs	1.60±0.22a	1.49±0.32a
肾脏Kidneys	0.46±0.04a	0.50±0.09a

项目 Items	下丘脑GHRH Hypothalamus GHRH	垂体GH Pituitarium GH	肝脏GHR Liver GHR	肝脏IGF-1 Liver IGF-1	十二指肠 GHR Duodenum GHR	十二指肠 IGF-1 Duodenum IGF-1	十二指肠 IGF-1R Duodenum IGF-1R
宰前活质量Slaughter live weight	-0.591	0.549	-0.611	-0.712	0.570	-0.445	-0.673
平均日增质量ADG	-0.933^**	0.831^*	-0.820^*	-0.694	0.850^*	-0.913^*	-0.765
采食量Feeding intake	-0.217	0.123	-0.025	0.041	0.062	-0.227	-0.237
头质量Head weight	0.508	-0.449	0.532	0.640	-0.486	0.340	0.639
心脏质量Heart weight	0.579	-0.660	0.768	0.882^*	-0.708	0.485	0.511
肝脏质量Liver weight	0.068	0.072	0.005	-0.089	-0.016	-0.020	0.233
脾脏质量Spleen weight	0.241	-0.427	0.387	0.197	-0.406	0.364	0.167
肺脏质量Lungs weight	0.366	-0.146	0.178	-0.026	-0.222	0.281	0.541
肾脏质量Kidneys weight	-0.242	0.307	-0.238	-0.284	0.231	-0.258	-0.206

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