Effect of Southern Blight Disease on Endogenous Hormone Content and Related Gene Expression Levels in Konjac

doi:10.7668/hbnxb.20195419

Abstract

Abstract:

To understand the changes in endogenous hormones and related gene expression levels after konjac infection with southern blight disease and reveal the main hormone pathways involved in konjac's response to southern blight disease,the ultra performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS) was employed to detect the changes in the contents of phytohormone (auxin,abscisic acid,trans-zeatin nucleoside,jasmonic acid,and salicylic acid) in the three-month-old Amorphophallus muelleri,which was infected with southern blight disease for 0,1,3 and 6 days.Moreover,the gene expression levels of abscisic acid,jasmonic acid,and salicylic acid pathways was analyzed by Real-time Quantitative PCR.The results showed that southern blight disease caused changes in the levels of various endogenous hormones in konjac.The content of indole-3-acetic acid tended to increased and then decreased with the southern blight disease infection,while the content of indole-3-butyric acid showed tended to decrease;the content of trans-zeatin nucleoside significant decreased with the infection of southern blight disease;the content of abscisic acid showed a significant increase followed by a decrease with the infection of southern blight disease;the content of jasmonic acid metabolites and salicylic acid metabolites were significantly increased than the control group with the infection of southern blight disease.The content of jasmonic acid of konjac infected with southern blight disease 1,3,and 6 d was 2.31,2.31,and 5.08 times that of the control group,and the content of salicylic acid was 5.53,4.60,and 7.38 times that of the control group,respectively.Eight genes related to abscisic acid,jasmonic acid,and salicylic acid pathways were activated and participated in the response process of konjac to southern blight disease.The above results indicated that the endogenous hormone homeostasis was disrupted after konjac infection with southern blight disease,activated the expression of plant hormone pathway related genes,and jasmonic acid and salicylic acid might play an important role in konjac's resistance to southern blight disease.

Key words: Amorphophallus muelleri, Southern blight disease, Plant hormone, Gene expression

CLC Number:

S436.32

GAO Penghua, QI Ying, YANG Min, LI Lifang, HUANG Feiyan, LIU Jiani, ZHAO Jianrong, YU Lei. Effect of Southern Blight Disease on Endogenous Hormone Content and Related Gene Expression Levels in Konjac[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 149-156. doi: 10.7668/hbnxb.20195419.

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References 49

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引物名称 Primer name	引物序列(5'—3') Primer sequence (5'—3')	产物大小/bp Product length
PP2C-F	ATGCCTGATCCTTGCGAGTG	140
PP2C-R	CAGGATCAGCTCCACCTCTC
ABF-F	AGAGCAAGCAGAAGGGAACC	107
ABF-R	CGGCTGCTTTACCTCCATCA
AOC-F	ATGGGAACCTGAAGAAGCGG	100
AOC-R	CTGTAGATGGCCTCGTAGCG
OPR4-F	GACAAGGAGGAGGGCAACAG	112
OPR4-R	GCGCGTTGATCTGAAACCTC
ACX-F	AGATTTGCCGCTTTCTTGGC	190
ACX-R	GTAGAAGCCGCCGTTGATGA
NPR-F	ATGAGAACAGTGGAGCTGGG	196
NPR-R	CGCTCTCCGCCTTATCCTTG
JAZ-F	GAGACGGGACTTGACGGAAC	118
JAZ-R	CCAAGAACCTCTGGAGCGAC
TGA1-F	CGAGTTCTTCCGCCAGGTC	162
TGA1-R	GGCGAGAACCCATTGTAGGC
Actin-F	GGTCCTGTTCCAGCCATCTC	142
Actin-R	GAACATGGTAGACCCTCCGC

引物名称 Primer name	引物序列(5'—3') Primer sequence (5'—3')	产物大小/bp Product length
PP2C-F	ATGCCTGATCCTTGCGAGTG	140
PP2C-R	CAGGATCAGCTCCACCTCTC
ABF-F	AGAGCAAGCAGAAGGGAACC	107
ABF-R	CGGCTGCTTTACCTCCATCA
AOC-F	ATGGGAACCTGAAGAAGCGG	100
AOC-R	CTGTAGATGGCCTCGTAGCG
OPR4-F	GACAAGGAGGAGGGCAACAG	112
OPR4-R	GCGCGTTGATCTGAAACCTC
ACX-F	AGATTTGCCGCTTTCTTGGC	190
ACX-R	GTAGAAGCCGCCGTTGATGA
NPR-F	ATGAGAACAGTGGAGCTGGG	196
NPR-R	CGCTCTCCGCCTTATCCTTG
JAZ-F	GAGACGGGACTTGACGGAAC	118
JAZ-R	CCAAGAACCTCTGGAGCGAC
TGA1-F	CGAGTTCTTCCGCCAGGTC	162
TGA1-R	GGCGAGAACCCATTGTAGGC
Actin-F	GGTCCTGTTCCAGCCATCTC	142
Actin-R	GAACATGGTAGACCCTCCGC

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