Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (3): 186-192. doi: 10.7668/hbnxb.20192715

Special Issue: Tomato Sweet pepper Plant protection Biotechnology

• Resources & Environment · Plant Protection • Previous Articles     Next Articles

Cloning,Subcellular Positioning of Pepper CaWRKY30 Transcription Factor, and Its Expression Analysis by Tomato spotted wilt orthotospovirus Infected

JIA Zhiqiang1, XU Yunyu1, GAO Xue2, TAO Hongzheng1,3, CHEN Zengmin4, LIU Yating1, , LI Yongzhong4,   

  1. 1. College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China
    2. College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China
    3. College of Life Science and Technology,Honghe University,Mengzi 661199,China
    4. College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China
  • Received:2022-02-26 Published:2022-06-30
  • Contact: LIU Yating, LI Yongzhong


In order to study the response mechanism of pepper CaWRKY30 transcription factor and Tomato spotted wilt orthotospovirus,it was experimental materials with pepper Xiangyan 11.The CaWRKY30 coding sequence was obtained by RNA extraction,RT-PCR,split gel and cloning.Biological information analysis results showed that CaWRKY30 full length was 1 122 bp,encoding 373 amino acids,the gene encoded protein contains 1 WRKY conservative domain and 1 C2H2 domain,belonged to a typical Ⅱ(e)subfamily member.System evolution analysis showed that the relative relationship with the potato StWRKY22 amino acid sequence was recently.It was found that CaWRKY30 was positioned in the nucleus and cell membranes in its cigarette seedlings,and leads to cell membranes.The results of Real-time fluorescence quantitative PCR analysis showed that the viral accumulation of Tomato spotted wilt orthotospovirus mechanical friction-vaccination was found that the viral accumulation was gradually increased from 1 to 14 days after inoculation,and virus accumulation reached its maximum in 14 days,after inoculation 14 days,viral accumulation gradually declined.At the same time,CaWRKY30 was induced by Tomato spotted wilt orthotospovirus,when the inoculation 1-14 days,the CaWRKY30 expression was raised,and the peak was reached in 14 days,the expression in 14 days gradually decreased.In summary,it obtained the CaWRKY30 transcription factor gene sequence,which was located in the nucleus and cell membrane,and preliminarily explained the expression trend of CaWRKY30 transcription factors under the stress of Tomato spotted wilt orthotospovirus.

Key words: Pepper, CaWRKY30 gene cloning, Bioinformatics, Tomato spotted wilt orthotospovirus, Expression analysis

Cite this article

JIA Zhiqiang, XU Yunyu, GAO Xue, TAO Hongzheng, CHEN Zengmin, LIU Yating, LI Yongzhong. Cloning,Subcellular Positioning of Pepper CaWRKY30 Transcription Factor, and Its Expression Analysis by Tomato spotted wilt orthotospovirus Infected[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 186-192. doi: 10.7668/hbnxb.20192715.

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