Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (4): 71-81. doi: 10.7668/hbnxb.20192961

Special Issue: Saline-alkali stress Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Identification of Kenaf WRKY Gene Family and Its Expression Analysis under Salt Stress

LI Hui1,2, KANG Zepei1, QIU Caisheng1, DAI Zhigang1, , QIU Huajiao1,   

  1. 1 Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China
    2 College of Life and Environment Science,Hunan University of Arts and Science,Changde 415000,China
  • Received:2022-02-19 Published:2022-08-28
  • Contact: DAI Zhigang, QIU Huajiao


To provide a solid foundation for studying the biological function of WRKY family members in kenaf in response to salt stress,all members of WRKY family were identified and their expression patterns were analyzed.Physical and chemical properties,phylogeny and conserved functional domains of WRKY gene family members were analyzed by bioinformatics method.The expression characteristics of WRKY gene family members under salt stress were analyzed by RT-PCR.The results showed that a total of 33 WRKY family members were identified,which were unevenly distributed on 12 chromosomes.There were certain differences in the physical and chemical properties of each member,such as amino acid number,molecular weight and theoretical isoelectric point.The conserved sequence WRKYGQK of each member did not change.Phylogenetic tree analysis showed that 33 WRKY family members were divided into 3 groups,GroupⅠ,GroupⅡ,Group Ⅲ,of which Group Ⅲ contained 5 subgroups.The real-time fluorescence quantitative RT-PCR results showed that there were 26 WRKY family members induced by salt stress,of which 23 had positive regulation and 3 had negative regulation.A total of 33 WRKY family members of kenaf were identified,of which 26 WRKY family members were involved in the salt stress response of kenaf.

Key words: Kenaf, WRKY, Bioinformatics, Salt stress

Cite this article

LI Hui, KANG Zepei, QIU Caisheng, DAI Zhigang, QIU Huajiao. Identification of Kenaf WRKY Gene Family and Its Expression Analysis under Salt Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 71-81. doi: 10.7668/hbnxb.20192961.

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