Identification of Kenaf WRKY Gene Family and Its Expression Analysis under Salt Stress

doi:10.7668/hbnxb.20192961

Abstract

Abstract:

To provide a solid foundation for studying the biological function of WRKY family members in kenaf in response to salt stress,all members of WRKY family were identified and their expression patterns were analyzed.Physical and chemical properties,phylogeny and conserved functional domains of WRKY gene family members were analyzed by bioinformatics method.The expression characteristics of WRKY gene family members under salt stress were analyzed by RT-PCR.The results showed that a total of 33 WRKY family members were identified,which were unevenly distributed on 12 chromosomes.There were certain differences in the physical and chemical properties of each member,such as amino acid number,molecular weight and theoretical isoelectric point.The conserved sequence WRKYGQK of each member did not change.Phylogenetic tree analysis showed that 33 WRKY family members were divided into 3 groups,GroupⅠ,GroupⅡ,Group Ⅲ,of which Group Ⅲ contained 5 subgroups.The real-time fluorescence quantitative RT-PCR results showed that there were 26 WRKY family members induced by salt stress,of which 23 had positive regulation and 3 had negative regulation.A total of 33 WRKY family members of kenaf were identified,of which 26 WRKY family members were involved in the salt stress response of kenaf.

Key words: Kenaf, WRKY, Bioinformatics, Salt stress

LI Hui, KANG Zepei, QIU Caisheng, DAI Zhigang, QIU Huajiao. Identification of Kenaf WRKY Gene Family and Its Expression Analysis under Salt Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 71-81. doi: 10.7668/hbnxb.20192961.

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URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192961

http://www.hbnxb.net/EN/Y2022/V37/I4/71

Figures/Tables 13

References 30

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登录号 Gene ID	基因名称 Gene name	引物序列(5'-3') Primer sequence(5'-3')
GWHGACDB035431	HcWRKY2	CTTTCCTTCCAGGGCAACAGTTGATAGATTGACGATGACGAGGC
GWHGACDB047709	HcWRKY3	CTATAGGTGGCGCAAATATGGACACTGTTGTTGACTGTGTTGTGGCT
GWHGACDB008458	HcWRKY4	TTTAGTGTGTTTTCACCTGGTCCCCTGTTTCTGCCCATACTTCCGCC
GWHGACDB001034	HcWRKY7	CTCCGCAGAAGATTGAAGAGTTGATGTGTGGGGCAAAATGGCTT
GWHGACDB042268	HcWRKY9	CAAAGACACCCAAACAACTCAGCCCTCCCACTCTCAATCTCCCTACCAC
GWHGACDB058507	HcWRKY11	GTTGTTGGTCAAAGTTGGGGGTACTTCCTCCAAGAATACTCGTCGG
GWHGACDB054546	HcWRKY13	GACCCCATCTCCTTTCCTTGCGTGTGTTCTTCACCACTTTCTGCC
GWHGACDB030263	HcWRKY17	CGTTTATGTCTTCTGCCATCACCTCACCCTATTCAACCTTCGCTTT
GWHGACDB063393	HcWRKY24	GTTAAGAACAACAAATTCCCAAGGTTTCCAACTCCAAAACATACCATC
GWHGACDB033458	HcWRKY31	CGGCAACAATAAAAATCAGCACAGCATTACACGATACTCCAACAT
GWHGACDB005801	HcWRKY32	GAAGCAAGTGAAGAGTCCAAAAGGTTGAAACAGGAACAGCAGACGATAA
GWHGACDB029256	HcWRKY33	ATCATCAAGCGAAGAGAAGAAAAATTAGAGCCGTGTAGGAATGTGTATCA
GWHGACDB051574	HcWRKY39	CAGGTAAGGGACAAGATGGGAGTACGGATTGAAGGAATGAGAGGGT
GWHGACDB057468	HcWRKY40-1	AAGGATGGATACCAATGGAGGAAATCCACTTGAGAAGGGGGGAGATG
GWHGACDB058543	HcWRKY40-2	CGAAGGAGAACACAACCACACACCGCCATTTGCTGAACCA
GWHGACDB060959	HcWRKY43	AGACCGAGAATGGCAACAGGGACTTGGATAATTGCTGTTTTTGACGGC
GWHGACDB050475	HcWRKY44	TCCCGTCGTATGCTCATTATTTTTAACTTCCTTTCACTTGTTTCTGTCCG
GWHGACDB005326	HcWRKY47	TATGGTCAGAAGATGGCAAAGGGTCGACGATGCGTAAGGTAAGGAA
GWHGACDB038970	HcWRKY53	CCTCGGGAGTGTCAGAAAATCAACACAGGAGGTGAAAAGACAAGGC
GWHGACDB008450	HcWRKY57	TTACCCACCACCCAGTTGCCTGCCACCGCCAATCCATACT
GWHGACDB053829	HcWRKY58	AGCCTCAACCAAATAAGCAAGCACACTTCATCACATCCTTCCTCGC
GWHGACDB062797	HcWRKY65	GGATAACGGGGAGGCGGATTGGTGCCGAAACACAAACGAG
GWHGACDB041115	HcWRKY71-1	GGCTTTTGGTCTGTCACCGTCCTCCCTTTCGTTTTCGCTTTGTTCCCT
GWHGACDB053781	HcWRKY72-1	GCCAATGGAGGAAATACGGACAAAGGTTGTGGTTGCCTTCGTAAGTTGT
GWHGACDB053987	HcWRKY72-2	TACATTTCCAGCGGGTTCCTCTTGCCCCAAACAGATGGTAGAA
GWHGACDB036356	HcWRKY74	AGGTAAGGGACAAGATGGCAGAACCTTGTTACTAATAGCAGGCACT
内参基因	Hcβaction	TTGCAGACCGTATGAGCAAGATCCTCCGATCCAGACACTG
Reference gene

登录号 Gene ID	基因名称 Gene name	引物序列(5'-3') Primer sequence(5'-3')
GWHGACDB035431	HcWRKY2	CTTTCCTTCCAGGGCAACAGTTGATAGATTGACGATGACGAGGC
GWHGACDB047709	HcWRKY3	CTATAGGTGGCGCAAATATGGACACTGTTGTTGACTGTGTTGTGGCT
GWHGACDB008458	HcWRKY4	TTTAGTGTGTTTTCACCTGGTCCCCTGTTTCTGCCCATACTTCCGCC
GWHGACDB001034	HcWRKY7	CTCCGCAGAAGATTGAAGAGTTGATGTGTGGGGCAAAATGGCTT
GWHGACDB042268	HcWRKY9	CAAAGACACCCAAACAACTCAGCCCTCCCACTCTCAATCTCCCTACCAC
GWHGACDB058507	HcWRKY11	GTTGTTGGTCAAAGTTGGGGGTACTTCCTCCAAGAATACTCGTCGG
GWHGACDB054546	HcWRKY13	GACCCCATCTCCTTTCCTTGCGTGTGTTCTTCACCACTTTCTGCC
GWHGACDB030263	HcWRKY17	CGTTTATGTCTTCTGCCATCACCTCACCCTATTCAACCTTCGCTTT
GWHGACDB063393	HcWRKY24	GTTAAGAACAACAAATTCCCAAGGTTTCCAACTCCAAAACATACCATC
GWHGACDB033458	HcWRKY31	CGGCAACAATAAAAATCAGCACAGCATTACACGATACTCCAACAT
GWHGACDB005801	HcWRKY32	GAAGCAAGTGAAGAGTCCAAAAGGTTGAAACAGGAACAGCAGACGATAA
GWHGACDB029256	HcWRKY33	ATCATCAAGCGAAGAGAAGAAAAATTAGAGCCGTGTAGGAATGTGTATCA
GWHGACDB051574	HcWRKY39	CAGGTAAGGGACAAGATGGGAGTACGGATTGAAGGAATGAGAGGGT
GWHGACDB057468	HcWRKY40-1	AAGGATGGATACCAATGGAGGAAATCCACTTGAGAAGGGGGGAGATG
GWHGACDB058543	HcWRKY40-2	CGAAGGAGAACACAACCACACACCGCCATTTGCTGAACCA
GWHGACDB060959	HcWRKY43	AGACCGAGAATGGCAACAGGGACTTGGATAATTGCTGTTTTTGACGGC
GWHGACDB050475	HcWRKY44	TCCCGTCGTATGCTCATTATTTTTAACTTCCTTTCACTTGTTTCTGTCCG
GWHGACDB005326	HcWRKY47	TATGGTCAGAAGATGGCAAAGGGTCGACGATGCGTAAGGTAAGGAA
GWHGACDB038970	HcWRKY53	CCTCGGGAGTGTCAGAAAATCAACACAGGAGGTGAAAAGACAAGGC
GWHGACDB008450	HcWRKY57	TTACCCACCACCCAGTTGCCTGCCACCGCCAATCCATACT
GWHGACDB053829	HcWRKY58	AGCCTCAACCAAATAAGCAAGCACACTTCATCACATCCTTCCTCGC
GWHGACDB062797	HcWRKY65	GGATAACGGGGAGGCGGATTGGTGCCGAAACACAAACGAG
GWHGACDB041115	HcWRKY71-1	GGCTTTTGGTCTGTCACCGTCCTCCCTTTCGTTTTCGCTTTGTTCCCT
GWHGACDB053781	HcWRKY72-1	GCCAATGGAGGAAATACGGACAAAGGTTGTGGTTGCCTTCGTAAGTTGT
GWHGACDB053987	HcWRKY72-2	TACATTTCCAGCGGGTTCCTCTTGCCCCAAACAGATGGTAGAA
GWHGACDB036356	HcWRKY74	AGGTAAGGGACAAGATGGCAGAACCTTGTTACTAATAGCAGGCACT
内参基因	Hcβaction	TTGCAGACCGTATGAGCAAGATCCTCCGATCCAGACACTG
Reference gene

登录号 Gene ID	基因名称 Gene name	起始位置 Start	终止位置 End	定位 Position	结构域 Domain pattern	数目/个 Number	等电点 pI	分子质量/ku Molecular weight
GWHGACDB035431	HcWRKY2	43156889	43163885	Chr2	WRKYGQK	753	6.31	82.1
GWHGACDB047709	HcWRKY3	18360711	18363601	Chr5	WRKYGQK	516	7.75	56.1
GWHGACDB008458	HcWRKY4	57427550	57429557	Chr10	WRKYGQK	487	7.19	52.4
GWHGACDB001034	HcWRKY7	10295072	10296202	Chr1	WRKYGQK	304	9.63	35.2
GWHGACDB042268	HcWRKY9	608483	610180	Chr4	WRKYGQK	442	6.34	48.9
GWHGACDB058507	HcWRKY11	60534662	60535249	Chr7	WRKYGQK	100	9.52	12.1
GWHGACDB054546	HcWRKY13	9913278	9916150	Chr7	WRKYGQK	228	9.35	26.6
GWHGACDB030263	HcWRKY17	1784934	1786015	Chr18	WRKYGQK	296	9.62	32.3
GWHGACDB033127	HcWRKY23	1268099	1269158	Chr2	WRKYGQK	271	9.76	31.3
GWHGACDB063393	HcWRKY24	9047246	9048493	Chr9	WRKYGQK	168	9.58	19.7
GWHGACDB028048	HcWRKY26	8797376	8799519	Chr17	WRKYGQK	511	8.86	57.6
GWHGACDB033458	HcWRKY31	3421809	3424016	Chr2	WRKYGQK	568	10.03	65.1
GWHGACDB005801	HcWRKY32	2994530	2996510	Chr10	WRKYGQK	337	9.41	37.9
GWHGACDB029256	HcWRKY33	38823602	38833317	Chr17	WRKYGQK	1 142	9.26	132.7
GWHGACDB051574	HcWRKY39	41780111	41781511	Chr6	WRKYGQK	335	9.52	38.1
GWHGACDB057468	HcWRKY40-1	53662855	53664154	Chr7	WRKYGQK	298	8.66	33.3
GWHGACDB058543	HcWRKY40-2	60735546	60739890	Chr7	WRKYGQK	521	9.74	58.5
GWHGACDB060959	HcWRKY43	22656914	22657706	Chr8	WRKYGQK	199	9.82	23.5
GWHGACDB050475	HcWRKY44	9983450	9985397	Chr6	WRKYGQK	475	9.37	53.6
GWHGACDB029897	HcWRKY46	44264516	44265589	Chr17	WRKYGQK	294	10.18	33.9
GWHGACDB005326	HcWRKY47	77210538	77212993	Chr1	WRKYGQK	497	9.22	55.9
GWHGACDB063681	HcWRKY51	12928402	12929691	Chr7	WRKYGQK	87	8.55	10.2
GWHGACDB038970	HcWRKY53	19064728	19065886	Chr3	WRKYGQK	329	5.26	37.1
GWHGACDB008450	HcWRKY57	57365676	57372205	Chr10	WRKYGQK	350	8.69	39.0
GWHGACDB053829	HcWRKY58	61029866	61032382	Chr6	WRKYGQK	522	9.01	58.8
GWHGACDB062797	HcWRKY65	3073815	3074868	Chr9	WRKYGQK	279	5.52	30.3
GWHGACDB041115	HcWRKY71-1	64485185	64486562	Chr3	WRKYGQK	311	6.89	34.7
GWHGACDB041095	HcWRKY71-2	64342462	64343984	Chr3	WRKYGQK	356	7.03	40.2
GWHGACDB053781	HcWRKY72-1	60729882	60731531	Chr6	WRKYGQK	474	5.37	51.3
GWHGACDB053987	HcWRKY72-2	61929508	61931157	Chr6	WRKYGQK	461	8.80	51.2
GWHGACDB036356	HcWRKY74	62394117	62395678	Chr2	WRKYGQK	321	9.52	36.2
GWHGACDB032609	HcWRKY75-1	40498829	40500411	Chr18	WRKYGQK	163	9.69	20.3
GWHGACDB043034	HcWRKY75-2	6956269	6958298	Chr4	WRKYGQK	169	9.51	20.1

登录号 Gene ID	基因名称 Gene name	起始位置 Start	终止位置 End	定位 Position	结构域 Domain pattern	数目/个 Number	等电点 pI	分子质量/ku Molecular weight
GWHGACDB035431	HcWRKY2	43156889	43163885	Chr2	WRKYGQK	753	6.31	82.1
GWHGACDB047709	HcWRKY3	18360711	18363601	Chr5	WRKYGQK	516	7.75	56.1
GWHGACDB008458	HcWRKY4	57427550	57429557	Chr10	WRKYGQK	487	7.19	52.4
GWHGACDB001034	HcWRKY7	10295072	10296202	Chr1	WRKYGQK	304	9.63	35.2
GWHGACDB042268	HcWRKY9	608483	610180	Chr4	WRKYGQK	442	6.34	48.9
GWHGACDB058507	HcWRKY11	60534662	60535249	Chr7	WRKYGQK	100	9.52	12.1
GWHGACDB054546	HcWRKY13	9913278	9916150	Chr7	WRKYGQK	228	9.35	26.6
GWHGACDB030263	HcWRKY17	1784934	1786015	Chr18	WRKYGQK	296	9.62	32.3
GWHGACDB033127	HcWRKY23	1268099	1269158	Chr2	WRKYGQK	271	9.76	31.3
GWHGACDB063393	HcWRKY24	9047246	9048493	Chr9	WRKYGQK	168	9.58	19.7
GWHGACDB028048	HcWRKY26	8797376	8799519	Chr17	WRKYGQK	511	8.86	57.6
GWHGACDB033458	HcWRKY31	3421809	3424016	Chr2	WRKYGQK	568	10.03	65.1
GWHGACDB005801	HcWRKY32	2994530	2996510	Chr10	WRKYGQK	337	9.41	37.9
GWHGACDB029256	HcWRKY33	38823602	38833317	Chr17	WRKYGQK	1 142	9.26	132.7
GWHGACDB051574	HcWRKY39	41780111	41781511	Chr6	WRKYGQK	335	9.52	38.1
GWHGACDB057468	HcWRKY40-1	53662855	53664154	Chr7	WRKYGQK	298	8.66	33.3
GWHGACDB058543	HcWRKY40-2	60735546	60739890	Chr7	WRKYGQK	521	9.74	58.5
GWHGACDB060959	HcWRKY43	22656914	22657706	Chr8	WRKYGQK	199	9.82	23.5
GWHGACDB050475	HcWRKY44	9983450	9985397	Chr6	WRKYGQK	475	9.37	53.6
GWHGACDB029897	HcWRKY46	44264516	44265589	Chr17	WRKYGQK	294	10.18	33.9
GWHGACDB005326	HcWRKY47	77210538	77212993	Chr1	WRKYGQK	497	9.22	55.9
GWHGACDB063681	HcWRKY51	12928402	12929691	Chr7	WRKYGQK	87	8.55	10.2
GWHGACDB038970	HcWRKY53	19064728	19065886	Chr3	WRKYGQK	329	5.26	37.1
GWHGACDB008450	HcWRKY57	57365676	57372205	Chr10	WRKYGQK	350	8.69	39.0
GWHGACDB053829	HcWRKY58	61029866	61032382	Chr6	WRKYGQK	522	9.01	58.8
GWHGACDB062797	HcWRKY65	3073815	3074868	Chr9	WRKYGQK	279	5.52	30.3
GWHGACDB041115	HcWRKY71-1	64485185	64486562	Chr3	WRKYGQK	311	6.89	34.7
GWHGACDB041095	HcWRKY71-2	64342462	64343984	Chr3	WRKYGQK	356	7.03	40.2
GWHGACDB053781	HcWRKY72-1	60729882	60731531	Chr6	WRKYGQK	474	5.37	51.3
GWHGACDB053987	HcWRKY72-2	61929508	61931157	Chr6	WRKYGQK	461	8.80	51.2
GWHGACDB036356	HcWRKY74	62394117	62395678	Chr2	WRKYGQK	321	9.52	36.2
GWHGACDB032609	HcWRKY75-1	40498829	40500411	Chr18	WRKYGQK	163	9.69	20.3
GWHGACDB043034	HcWRKY75-2	6956269	6958298	Chr4	WRKYGQK	169	9.51	20.1

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