Expression Pattern Analysis of Auxin Response Factor Genes in Tuber Mustard under Stresses Treatment

doi:10.7668/hbnxb.20192377

Abstract

Abstract: To identify the candidate auxin response factors gene(ARF) responsive to salt and Plasmodiophora brassicae stresses in tuber mustard,and provide a basis for further studying their functions in the resistance of tuber mustard to stresses.Bioinformatic assay was used to analyze the cis-acting elements in the promoters of ARF family genes,and the fluorescent quantitative PCR method was used to analyze the expression patterns of ARF in different organs,as well as their expression levels under salt and Plasmodiophora brassicae stresses.The results showed that several cis-acting elements related to auxin,salt and pathogen stresses were found in the promoters of ARF genes. BjARF1A and BjARF4B were highly expressed in the leaves,and BjARF7C was highly expressed in the roots.After 3 h of salt stress treatment,the expression of BjARF1B and BjARF2B in root were severely induced;after 6 h of salt stress treatment,the expression of BjARF9A and BjARF13E were also significantly induced.Under the pathogen Plasmodiophora brassicae treatment,the expression of BjARF3A and BjARF3D were highly induced by the pathogen at 12,24 h,respectively;while the expression level of BjARF13B were continuously decreased,and the degree of decline gradually increased.In together,four salt stress responsive ARF genes and three Plasmodiophora brassicae responsive ARF genes were found,laying a foundation for the further study of ARF family gene functions.

Key words: Tuber mustard, Auxin response factor(ARF), Salt stress, Plasmodiophora brassicae, Gene expression analysis

CLC Number:

S637.03
Q78

CAI Zhaoming, CHENG Chunhong, FU Min, WANG Diandong. Expression Pattern Analysis of Auxin Response Factor Genes in Tuber Mustard under Stresses Treatment[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 7-18. doi: 10.7668/hbnxb.20192377.

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