Physiological Responses and Salt Tolerance Mechanisms of Sweet Cherry Scion-Rootstock Combinations under Salt Stress

doi:10.7668/hbnxb.20196271

Abstract

Abstract:

The study aimed to examine the differences in physiological responses to NaCl between these two sweet cherry scion-rootstock combinations,explore the physiological mechanisms governing how different sweet cherry scion-rootstock combinations respond to salt stress,and provide a basis for screening salt-tolerant rootstocks and cultivating sweet cherries in saline-alkali soils.One-year-old plants of the sweet cherry cultivar Meizao(M) grafted onto Gisela 6 (G6) and Colt (KT) rootstocks were used as experimental materials.A pot culture experiment was conducted to simulate NaCl stress,with determinations made on the contents of osmotic adjustment substances,activities of antioxidant enzymes,and photosynthetic gas exchange parameters.The results showed that NaCl treatment promoted the accumulation of osmotic adjustment substances such as soluble sugar,soluble protein,and proline in the leaves of the two sweet cherry scion-rootstock combinations.With the increase in NaCl treatment concentration,the soluble sugar content of M/G6 increased first and then decreased,while that of M/KT continued to increase.Under the 150 mmol/L NaCl treatment,the increase in proline content of M/KT (6.11 times) was significantly higher than that of M/G6 (1.74 times).The contents of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) increased with the increase in NaCl treatment concentration.However,the peroxidase (POD) activity of M/G6 reached its peak at 100 mmol/L,which was significantly higher than that of M/KT.With the increase in NaCl treatment concentration,the net photosynthetic rate (Pn) and stomatal conductance (Gs) of the leaves of the two sweet cherry scion-rootstock combinations gradually decreased,while the intercellular CO₂ concentration (Ci) gradually increased.Non-stomatal limitation was the main factor for the decrease in Pn of the leaves of the two scion-rootstock combinations.M/G6 showed better salt tolerance under moderate salt stress (100 mmol/L),alleviating oxidative damage by relying on the accumulation of soluble sugar and the enhancement of POD activity.M/KT maintained osmotic balance through the accumulation of proline and the increase in SOD activity under high salt stress (150 mmol/L).Based on comprehensive evaluation,in actual production,M/KT can be considered as a reference option for slightly saline soil,and M/G6 is recommended for moderately saline soil.

Key words: Sweet cherry, Salt stress, Scion-rootstock combinations, Physiological characteristics, Photosynthetic characteristics

CLC Number:

S662.5

LI Huanyong, LIAO Fangzhou, WANG Fei, WANG Yaodong, XU Zhigang, ZHANG Yao, YANG Lifang. Physiological Responses and Salt Tolerance Mechanisms of Sweet Cherry Scion-Rootstock Combinations under Salt Stress[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 110-116. doi: 10.7668/hbnxb.20196271.

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Figures/Tables 4

Fig.1 Plant morphological changes and leaf phenotypic changes of sweet cherry seedlings grafted with two rootstocks under salt stress A.M/G6,B.M/KT.0,50,100 and 150 indicate 0,50,100,and 150 mmol/L NaCl treatment,respectively.

Fig.2 Changes of the contents of osmotic adjustment substances in sweet cherry seedlings grafted with two rootstocks under salt stress Different small letters indicate significant differences among different treatments of the same variety (P<0.05).The same as Fig.3,4.

Fig.3 Changes of the MDA content and SOD and POD activity in sweet cherry seedlings grafted with two rootstocks under salt stress

Fig.4 Changes of the photosynthetic gas exchange parameters in sweet cherry seedlings grafted with two rootstocks under salt stress

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