Effects of Three Exogenous Amino Acids on the Resistance of Tomato Seedlings to High-temperature Stress

doi:10.7668/hbnxb.20195734

Abstract

Abstract:

This study aimed to investigate the impacts of three exogenous amino acids on the growth and development of tomato seedlings under high-temperature stress.Tomato seedlings were selected as the study material.The normal temperature treatment with clean water at 25 ℃/18 ℃(day/night)and the high-temperature treatment with clean water at 40 ℃/30 ℃(day/night)served as control and heat groups,respectively.Under high-temperature,tomato seedlings were sprayed with 5 mmol/L γ-aminobutyric acid(GABA),0.2 mmol/L 5-aminolevulinic acid(ALA),and 5 mmol/L Proline(Pro)on the leaves once every seven days for two times.Growth parameters,antioxidant enzyme activities,and physiological parameters were determined.The results indicated that the plant height,stem diameter,and biomass accumulation of tomato seedlings was significantly reduced under high temperature.Compared with heat treatment,spraying GABA,ALA,and Pro significantly enhanced the plant height and stem thickness of tomato seedlings,promoted root development,increased biomass accumulation and relative growth rate under high-temperature.Moreover,the chlorophyll content and net photosynthetic rate of tomato seedlings declined remarkably.The chlorophyll content in the ALA treatment was higher than that in other treatments,while the net photosynthetic rate in the GABA treatment was the optimal.Compared with heat treatment,spraying GABA and ALA significantly augmented the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT).High-temperature stress substantially elevated the relative electrical conductivity(REC)and malondialdehyde(MDA)content of leaves.Meanwhile,the leaves treated with Pro exhibited the lowest REC and MDA content,nearly returning to the level of control treatment.The outcomes of cluster and principal component analyses revealed that the GABA and ALA treatments mainly enhanced the photosynthetic rate and promoted plant growth by coordinately regulating the chlorophyll content and antioxidant enzyme activities of tomato seedlings,thereby strengthening the capacity to resist high-temperature stress.In contrast,Pro treatment effectively regulated the leaf's osmotic pressure by reducing leaf relative conductivity and MDA content.

Key words: Tomato, High-temperature stress, GABA, ALA, Proline, Growth, Physiological characteristics

CLC Number:

SHI Jianshuo, WANG Liying, JIANG Longgang, LI Ruonan, GUO Li, LI Yihong, PAN Lijia, HOU Shenglin, JIA Zhou. Effects of Three Exogenous Amino Acids on the Resistance of Tomato Seedlings to High-temperature Stress[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 117-126. doi: 10.7668/hbnxb.20195734.

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Figures/Tables 8

Fig.1 Effects of three exogenous amino acids on plant height and stem diameter of tomato seedlings at 7th day and at 15th day after treatments Different letters above the bars indicate significant differences between treatments(P<0.05).The same as Fig.2—7.

Fig.2 Effects of three exogenous amino acids on root growth of tomato seedlings at 15th day after treatments

Fig.3 Effects of three exogenous amino acids on aboveground fresh weight,aboveground dry weight, underground fresh weight,and underground dry weight of tomato seedlings at 7th day and at 15th day after treatments

Fig.4 Effects of three exogenous amino acids on seedling index and relative growth rate of tomato seedlings

Fig.5 Effects of three exogenous amino acids on chlorophyll content and net photosynthetic rate of tomato seedlings at 15th day after treatments

Fig.6 Effects of three exogenous amino acids on antioxidant enzyme activity of tomato seedling leaves at 15th day after treatments

Fig.7 Effects of three exogenous amino acids on REC and MDA content of tomato seedling leaves at 15th day after treatments

Fig.8 Comprehensive evaluation of alleviating high temperature stress by exogenous amino acids Clustering analysis and principal component analysis(PCA)were applied to the collected phenotype data of tomato seedlings at day 15 after treatments.PH.Plant height; MDA.Malondialdehyde; Chl.Total chlorophyll content; RGR.Relative growth rate; BDW.Root dry weight; SI.Sound seedling index; RD.Root average diameter; Chlb.Chlorophyll b content; ADW.Aboveground dry weight; RV.Root volume; Chla.Chlorophyll a content; Pn.Net photosynthetic rate; CAT.CAT activity;SD.Stem diameter; POD.POD activity; RS.Root surface area; SOD.SOD activity; RL.Root length.Data analysis was carried out via R programming.

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