Physiological Response of Different Heat-tolerant Watermelon Seedlings to High Temperature Stress

doi:10.7668/hbnxb.20194963

Abstract

Abstract:

In order to explore the effects of high temperature stress on different heat-tolerant watermelon inbred lines,heat-sensitive (D27) and heat-tolerant (K53) watermelon seedlings were treated at 42 ℃ for 48 h,and their phenotype,tissue structure,photosynthetic characteristics,antioxidant enzyme activities and osmotic regulators were measured and analyzed every 12 h.The results showed that the leaf thickness,fence tissue thickness,sponge tissue thickness and tissue compactness of heat-tolerant K53 were larger than those of heat-sensitive D27 after high temperature stress.The proportion of sponge tissue in the palisade tissue of D27 decreased more than that of K53.With the increase of high temperature stress time,the net photosynthetic rate (Pn),transpiration rate (Tr) and stomatal conductance (Gs) of the two inbred lines decreased,and the intercellular CO₂ concentration (Ci) increased.And the change amplitude of D27 was greater than that of K53.Among the four photosynthetic pigment contents,the heat-tolerant type was higher than the heat-sensitive type under high temperature stress at different treatment times.The superoxide dismutase (SOD) and peroxidase (POD) of the two inbred lines increased first and then decreased with the increase of high temperature stress time,and the enzyme activity was the highest at 24 h,and the enzyme activity of K53 was significantly higher than that of D27.After high temperature stress,the relative conductivity of the two inbred lines increased,and the relative conductivity of K53 increased less than that of D27.The malondialdehyde (MDA) content of D27 was reduced;the MDA content of K53 decreased after an increase.With the increase of high temperature stress,the soluble protein content and proline content (Pro) of K53 were significantly higher than those of D27 at 24 h.In summary,the heat-tolerant type K53 had a stronger resistance to high temperature stress than the heat-sensitive type D27.

Key words: Citrullus lanatus, Heat tolerance, High temperature stress, Anatomical structure, Physiological characteristics

ZHANG Liangge, SHI Wenxin, LI Ai, ZHANG Weihua, LIN Ping, XUE Jia. Physiological Response of Different Heat-tolerant Watermelon Seedlings to High Temperature Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(5): 117-127. doi: 10.7668/hbnxb.20194963.

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References 48

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高温胁迫处理时间/h Treatment time of high temperature stress	主脉直径/μm Main pulse diameter
高温胁迫处理时间/h Treatment time of high temperature stress	D27	K53
0	597.71±10.47b	569.43±18.89b
12	785.46±14.78a	781.91±7.98a
24	763.24±31.01a	772.49±17.58a
36	751.11±40.30a	730.63±15.88a
48	757.79±6.67a	754.33±3.96a

高温胁迫处理时间/h Treatment time of high temperature stress	主脉直径/μm Main pulse diameter
高温胁迫处理时间/h Treatment time of high temperature stress	D27	K53
0	597.71±10.47b	569.43±18.89b
12	785.46±14.78a	781.91±7.98a
24	763.24±31.01a	772.49±17.58a
36	751.11±40.30a	730.63±15.88a
48	757.79±6.67a	754.33±3.96a

结构参数 Structural parameters	材料 Material	高温胁迫处理时间/h Treatment time of high temperature stress
结构参数 Structural parameters	材料 Material	0	12	24	36	48
叶片厚度/μm	D27	152.90±3.18c	132.19±2.54d	138.78±1.82d	137.66±5.45d	113.54±3.11e
Vane thickness	K53	180.35±3.22a	169.81±3.33ab	169.06±4.42ab	161.14±4.62bc	160.21±2.58bc
上表皮厚度/μm	D27	13.03±0.27abc	10.91±0.39de	11.28±0.51cde	11.75±0.63bcde	10.42±0.55e
Upper skin thickness	K53	13.76±0.67a	13.32±0.68ab	13.73±0.75a	12.73±0.40abcd	11.57±0.68bcde
下表皮厚度/μm	D27	10.58±0.23a	9.00±0.38bc	9.66±0.48abc	9.37±0.38abc	8.86±0.38c
Lower skin thickness	K53	10.29±0.34ab	10.20±0.39abc	10.09±0.54abc	9.24±0.30bc	9.53±0.48abc
栅栏组织厚度/μm	D27	65.21±1.56ab	54.52±1.18de	58.71±0.95cd	51.95±1.72ef	30.93±1.18g
Palisade tissue thickness	K53	67.93±1.25a	66.91±1.73ab	62.67±2.26bc	47.53±1.30f	50.25±1.22ef
海绵组织厚度/μm	D27	55.57±1.60c	46.56±1.26d	52.09±1.00cd	49.18±2.34d	46.87±1.98d
Sponge tissue thickness	K53	73.41±1.94a	62.77±0.99b	65.06±2.61b	62.89±1.78b	65.50±3.06b
组织紧密度	D27	0.43±0.01a	0.42±0.01a	0.42±0.01ab	0.33±0.02de	0.28±0.01g
Tissue tightness	K53	0.37±0.01cd	0.38±0.01bc	0.33±0.02de	0.29±0.01fg	0.32±0.01ef
组织疏密度	D27	0.37±0.01bcd	0.36±0.01cd	0.37±0.01bcd	0.33±0.02d	0.37±0.02bcd
Tissue looseness	K53	0.45±0.01a	0.36±0.01bcd	0.39±0.02bc	0.41±0.02ab	0.41±0.02b
栅海比	D27	1.18±0.04ab	1.21±0.04a	1.15±0.04ab	1.03±0.06c	0.68±0.04f
Ratio of palisade tissue to	K53	0.85±0.04de	1.08±0.04bc	0.96±0.05cd	0.72±0.02f	0.75±0.05ef
spongy tissue

结构参数 Structural parameters	材料 Material	高温胁迫处理时间/h Treatment time of high temperature stress
结构参数 Structural parameters	材料 Material	0	12	24	36	48
叶片厚度/μm	D27	152.90±3.18c	132.19±2.54d	138.78±1.82d	137.66±5.45d	113.54±3.11e
Vane thickness	K53	180.35±3.22a	169.81±3.33ab	169.06±4.42ab	161.14±4.62bc	160.21±2.58bc
上表皮厚度/μm	D27	13.03±0.27abc	10.91±0.39de	11.28±0.51cde	11.75±0.63bcde	10.42±0.55e
Upper skin thickness	K53	13.76±0.67a	13.32±0.68ab	13.73±0.75a	12.73±0.40abcd	11.57±0.68bcde
下表皮厚度/μm	D27	10.58±0.23a	9.00±0.38bc	9.66±0.48abc	9.37±0.38abc	8.86±0.38c
Lower skin thickness	K53	10.29±0.34ab	10.20±0.39abc	10.09±0.54abc	9.24±0.30bc	9.53±0.48abc
栅栏组织厚度/μm	D27	65.21±1.56ab	54.52±1.18de	58.71±0.95cd	51.95±1.72ef	30.93±1.18g
Palisade tissue thickness	K53	67.93±1.25a	66.91±1.73ab	62.67±2.26bc	47.53±1.30f	50.25±1.22ef
海绵组织厚度/μm	D27	55.57±1.60c	46.56±1.26d	52.09±1.00cd	49.18±2.34d	46.87±1.98d
Sponge tissue thickness	K53	73.41±1.94a	62.77±0.99b	65.06±2.61b	62.89±1.78b	65.50±3.06b
组织紧密度	D27	0.43±0.01a	0.42±0.01a	0.42±0.01ab	0.33±0.02de	0.28±0.01g
Tissue tightness	K53	0.37±0.01cd	0.38±0.01bc	0.33±0.02de	0.29±0.01fg	0.32±0.01ef
组织疏密度	D27	0.37±0.01bcd	0.36±0.01cd	0.37±0.01bcd	0.33±0.02d	0.37±0.02bcd
Tissue looseness	K53	0.45±0.01a	0.36±0.01bcd	0.39±0.02bc	0.41±0.02ab	0.41±0.02b
栅海比	D27	1.18±0.04ab	1.21±0.04a	1.15±0.04ab	1.03±0.06c	0.68±0.04f
Ratio of palisade tissue to	K53	0.85±0.04de	1.08±0.04bc	0.96±0.05cd	0.72±0.02f	0.75±0.05ef
spongy tissue

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