Changes of Physiological Characteristics and Genetic Material Distortion of Naked Oats Seeds with Different Storage Years

doi:10.7668/hbnxb.20193229

Abstract

Abstract:

In order to explore the changes of physiological characteristics and genetic materials of naked oats during storage, the germination rate, germination potential, malondialdehyde(MDA)content, peroxidase dismutase(SOD)activity, catalase(CAT)activity, peroxidase(POD)activity and polyphenol oxidase(PPO)activity of naked oats seeds with different storage years were measured. The number of cells with genetic material distortion was counted by microscopic examination.The results showed that the germination rate and germination potential of naked oats seeds decreased with the extension of storage years.After storage for 8 years,Bayou 1 decreased by 86.42 and 96.92 percentage points;the content of MDA in naked oats seeds increased with the extension of storage years,and the maximum increase of Jinyan 8 was 5.68 times;the activities of SOD,CAT,POD and PPO of naked oats seeds decreased with the extension of storage years.After 8 years of storage,Bayou 1 decreased the most,which were 84.66%,74.38%,54.09% and 81.44% respectively;the number of cells with genetic material distortion in naked oats increased with the extension of storage years,and the average increase of the three varieties for 8 years was 11.87 times.The germination rate was significantly correlated with MDA content,SOD activity,CAT activity,POD activity,PPO activity and the number of aberrant cells(P<0.01),and the number of aberrant cells was significantly correlated with MDA content,SOD activity,CAT activity,POD activity and PPO activity(P<0.01).Bayou 1 had the best storage resistance,Jinyan 17 was the second,and Jinyan 8 was the worst.The germination rate and germination potential of naked oats decreased with the extension of storage years,the activities of SOD,CAT,POD and PPO decreased with the extension of storage years,and the content of MDA and the number of distorted cells increased with the extension of storage years;SOD,CAT and POD activities are essential enzymes for seed survival,but not the key enzymes for seed germination.

Key words: Naked oats, Storage life, Physiological characteristics, Genetic material distortion

ZHANG Qiaoxian, LI Qiang, LIU Xiaojie, TANG Yu. Changes of Physiological Characteristics and Genetic Material Distortion of Naked Oats Seeds with Different Storage Years[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 44-50. doi: 10.7668/hbnxb.20193229.

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References 24

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年限 Years	晋燕8号Jinyan 8
年限 Years	发芽率 Germination percentage	发芽势 Germination potential		发芽率 Germination percentage		发芽势 Germination potential		发芽率 Germination percentage		发芽势 Germination potential
0	90.54±4.15b		90.54±4.15b		96.31±2.42a		96.31±2.42ab		93.54±2.66a		90.88±2.12a
1	97.89±2.67a		97.89±2.67a		99.13±0.85a		99.13±0.85a		92.92±3.87a		92.92±3.87a
2	83.49±3.15b		83.49±3.15c		90.33±2.43b		90.33±2.43b		92.80±3.54a		92.80±3.54ab
3	73.41±5.89c		72.32±5.18d		78.33±3.21c		77.00±2.66c		88.57±2.46a		88.57±2.46b
8	30.54±2.56d		25.21±2.36e		41.39±3.87d		34.91±6.88d		12.70±7.73b		2.86±3.33c

年限 Years	晋燕8号Jinyan 8
年限 Years	发芽率 Germination percentage	发芽势 Germination potential		发芽率 Germination percentage		发芽势 Germination potential		发芽率 Germination percentage		发芽势 Germination potential
0	90.54±4.15b		90.54±4.15b		96.31±2.42a		96.31±2.42ab		93.54±2.66a		90.88±2.12a
1	97.89±2.67a		97.89±2.67a		99.13±0.85a		99.13±0.85a		92.92±3.87a		92.92±3.87a
2	83.49±3.15b		83.49±3.15c		90.33±2.43b		90.33±2.43b		92.80±3.54a		92.80±3.54ab
3	73.41±5.89c		72.32±5.18d		78.33±3.21c		77.00±2.66c		88.57±2.46a		88.57±2.46b
8	30.54±2.56d		25.21±2.36e		41.39±3.87d		34.91±6.88d		12.70±7.73b		2.86±3.33c

品种 Varieties	0		1		2		3		8
品种 Varieties	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential
晋燕8号Jinyan 8	90.54±4.15b	90.54±4.15b	97.89±2.67ab	97.89±2.67a	83.49±3.15b	83.49±3.15c	73.41±5.89c	72.32±5.18d	30.54±2.56d	25.21±2.36e
晋燕17号Jinyan 17	96.31±2.43a	96.31±2.42ab	99.13±0.85a	99.13±0.85a	90.33±2.43b	90.33±2.43b	78.33±3.21c	77.00±2.66c	41.39±3.87d	34.91±6.88d
坝莜1号Bayou 1	93.54±2.66a	93.54±2.66a	92.92±3.87a	92.92±3.87ab	92.80±3.54a	92.80±3.54ab	88.57±2.46a	88.57±2.46b	12.70±7.73b	2.86±3.33c

品种 Varieties	0		1		2		3		8
品种 Varieties	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential	发芽率 Germination percentage	发芽势 Germination potential
晋燕8号Jinyan 8	90.54±4.15b	90.54±4.15b	97.89±2.67ab	97.89±2.67a	83.49±3.15b	83.49±3.15c	73.41±5.89c	72.32±5.18d	30.54±2.56d	25.21±2.36e
晋燕17号Jinyan 17	96.31±2.43a	96.31±2.42ab	99.13±0.85a	99.13±0.85a	90.33±2.43b	90.33±2.43b	78.33±3.21c	77.00±2.66c	41.39±3.87d	34.91±6.88d
坝莜1号Bayou 1	93.54±2.66a	93.54±2.66a	92.92±3.87a	92.92±3.87ab	92.80±3.54a	92.80±3.54ab	88.57±2.46a	88.57±2.46b	12.70±7.73b	2.86±3.33c

因素 Factor	自由度 Degree of freedom	发芽率 Germination percentage	发芽势 Germination potential	MDA含量 MDA content	SOD活性 SOD activity	CAT活性 CAT activity	POD活性 POD activity	PPO活性 PPO activity	畸变细胞 Aberrant cell
品种Varieties	2	13.147^***	10.058^***	19.165^***	12.032^***	8.595^**	22.022^***	0.853ns	3.427^*
年限 Year	4	631.835^***	695.913^***	100.949^***	245.981^***	78.812^***	65.944^***	53.087^***	61.040^***
品种×贮藏年限Varieties×year	8	16.276^***	15.613^***	4.455^**	10.121^***	2.728^*	1.326ns	1.131ns	3.910^**

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