Effects of NaCl Stress on Growth and Physiological Characteristics of Sugar Beet at Different Seedling Stages

doi:10.7668/hbnxb.20190029

Abstract

Abstract: In order to investigate the adaptability and physiological response of sugar beet to NaCl stress, the effects of NaCl stress on growth and physiological characteristics of sugar beet seedlings were studied by pot culture. The results showed that with the increase of NaCl concentration at the same seedling stage, seedling reservation rate began to decrease at 0.6% NaCl concentration, and plant height decreased gradually. The dry weight of sugar beet showed a single peak curve change. Low salt stress was beneficial to the increase of sugar beet biomass, and the chlorophyll content increased. The plasmalemma permeability and MDA content of leaves increased, and the cell membrane system was damaged seriously. SOD and POD activities increased, and antioxidant enzyme activities enhanced. With the advance of seedling age, the differences of seedling reservation rate, plant height, dry weight, chlorophyll content, plasmalemma permeability of leaves and MDA content gradually decreased among NaCl stress treatments, while the differences of SOD activity and POD activity gradually increased, and the salt tolerance of sugar beet increased. In conclusion, with the increase of NaCl concentration, SOD and POD activities, chlorophyll content and plasmalemma permeability and MDA content of leaves increased, but seedling reservation rate, plant height and dry weight decreased. With the advance of seedling age, the inhibition of salt on the growth of sugar beet weakened.

Key words: Sugar beet, Seedling age, NaCl stress, Growth, Physiological characteristics

CLC Number:

S566.3

HUANG Chunyan, SU Wenbin, FAN Fuyi, GUO Xiaoxia, LI Zhi, JIAN Caiyuan, TIAN Lu, REN Xiaoyun, GONG Qianheng. Effects of NaCl Stress on Growth and Physiological Characteristics of Sugar Beet at Different Seedling Stages[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 163-169. doi: 10.7668/hbnxb.20190029.

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References

[1] Asish K P, Das A B. Salt tolerance and salinity effects on plants:a review[J]. Ecotoxicology and Environmental Safety, 2005, 60(3):324-349.doi:10.1016/j.ecoenv.2004.06.010.
[2] 史淑芝, 程大友, 马凤鸣, 崔杰. 生物质能源作物-能源甜菜的开发利用[J]. 中国农学通报, 2007, 23(11):416-419.
Si S Z, Chen D Y, Ma F M, Cui J. Exploration and utilization of biomass energy crop-energy beet[J]. Chinese Agricultural Science Bulletin,2007,23(11):416-419.
[3] Rengasamy P. Soil processes affecting crop production in salt-affected soils[J].Functional Plant Biology,2010,37(7):613-620.doi:10.1071/FP09249.
[4] Ashraf M. Some important physiological selection criteria for salt tolerance in plants[J].Flora, 2004,199(5):361-376.doi:10.1078/0367-2530-00165.
[5] Yuan Y H, Shu S, Li Shu H, He L Z, Li H, Du N, Sun J, Cuo S R. Effects of exogenous putrescine on chlorophyll fluorescence imaging and heat dissipation capacity in cucumber (Cucumis sativus L.) under salt stress[J].Journal of Plant Growth Regulation, 2014,33(44):798-808.doi:10.1007/s00344-014-9427-z.
[6] 陈建波, 王全喜, 章洁. 绿豆芽超氧化物歧化酶在胁迫条件下的活性变化[J]. 上海师范大学学报(自然科学版), 2007, 36(1):49-53.doi:10.3969/j.issn.1000-5137.2007.01.010.
Cen J B, Wang Q X, Zhang J. The change of superoxide dismutase's activity in bean sprout under stress circumstance[J]. Journal of Shanghai Normal University (Nature Science), 2007, 36(1):49-53.
[7] 黄春燕, 苏文斌, 张少英,樊福义, 郭晓霞, 李智, 菅彩媛, 任霄云, 宫前恒. 施钾量对膜下滴灌甜菜光合性能以及对产量和品质的影响[J]. 作物学报, 2018,44(10):1496-1505. doi:10.3724/SP.J.1006.2018.01496.
Hang C Y, Su W B, Zhang S Y, Fan F Y, Guo X X, Li Z, Jian C Y, Ren X Y, Gong Q H. Effects of potassium application on photosynthetic performance, yield, and quality of sugar beet with mulching-drip irrigation[J]. Acta Agronomica Sinica,2018,44(10):1496-1505.
[8] 吴晓雷, 田自华, 张家骅, 邵金旺. 甜菜抗盐生理研究进展[J].中国甜菜, 1991(2):46-49.
W X L, Tian Z H, Zhang J H, Shao J W.Advances in salt resistance physiology of sugarbeet[J].Sugar Crops China, 1991(2):46-49.
[9] 李志全.甜菜幼苗的耐盐限度[J].新疆农业科学, 1981(2):17-19.
L Z Q.Salt tolerance limits of sugarbeet seedlings[J].Xinjiang Agricultural Science, 1981(2):17-19.
[10] 惠菲. 盐胁迫下甜菜蛋白质含量测定及特异蛋白的分离与鉴定[D].哈尔滨:黑龙江大学, 2012.doi:10.7666/d.y2122454.
Hi F. Determination of protein content and isolation and identification of specific protein in sugarbeet under salt stress[D]. Harbin:Heilongjiang University,2012.
[11] Parida A K, Das A B. Salt tolerance and salityeffects on plants:a review[J]. Ecotoxicology and Environment Safety, 2005,60(3):324-349.doi:10.1016/j.ecoenv.2004.06.010.
[12] 彭云玲,保杰, 叶龙山, 王永健, 燕利斌. NaCl胁迫对不同耐盐型玉米自交系萌动种子和幼苗离子稳态的影响[J]. 生态学报,2014,34(24):7320-7328.doi:10.5846/stxb201303150431.
Png Y L, Bao J, Ye L S, Wang Y J, Yan L B. Ion homeostasis in germinating seeds and seedlings of three maize inbred lines under salt stress[J].Acta Ecologica Sinica, 2014,34(24):7320-7328.
[13] 刘文瑜, 杨发荣, 黄杰,魏玉明, 金茜. NaCl胁迫对藜麦幼苗生长和抗氧化酶活性的影响[J]. 西北植物学报, 2017,37(9):1797-1804.doi:10.7606/j.issn.1000-4025.2017.09.1797.
Lu W Y, Yang F R, Huang J, Wei Y M, Jin Q. Response of seedling growth and the activities of antioxidant enzymes of Chenopodium quinoa to salt stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017,37(9):1797-1804.
[14] 李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社, 2002:65.
L H S. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing:Higher Education Press, 2002:65.
[15] 钟全林, 程栋梁, 胡松竹, 贺利中, 唐承财,文雅香, 邱剑飞, 李秀花. 刨花楠和华东润楠叶绿素含量分异特征及与净光合速率的关系[J]. 应用生态学报, 2009,20(2):271-276.doi:10.13287/j.1001-9332.2009.0066.
Zong Q L, Cheng D L, Hu S Z, He L Z, Tang C C, Weng Y X, Qiu J F, Li X H. Chlorophyll content and net photosynthetic rate of Machiluspauhoi and M.leptophylla[J]. Chinese Journal of Applied Ecology,2009,20(2):271-276.
[16] Bielinis E, Jóźwiak W, Robakowski P.Modelling of the relationship between the SPAD values and photosynthetic pigments content in Quercus petraea and Prunuss erotina leaves[J].Dendrobiology, 2015, 73:125-134.doi:10.12657/denbio.073.013.
[17] 刘爱荣, 张远兵, 钟泽华, 吴夏, 张明. 盐胁迫对彩叶草生长和渗透调节物质积累的影响[J].草业学报, 2013,22(2):211-218.
Lu A R, Zhang Y B, Zhong Z H, Wu X, Zhang M. Effects of salt stress on the growth and osmotic accumulation of Coleus blumei[J]. Acta Prataculturae Sinica, 2013,22(2):211-218.
[18] Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry, 2010,48(12):909-930.doi:10.1016/j.plaphy.2010.08.016.
[19] 张冠初, 张智猛, 慈敦伟, 丁红, 杨吉顺, 史晓龙, 田家明, 戴良香.干旱和盐胁迫对花生渗透调节和抗氧化酶活性的影响[J].华北农学报, 2018,33(3):176-181.doi:10.7668/hbnxb.2018.03.026.
Zang G C, Zhang Z M, Ci D W, Ding H, Yang J S, Shi X L, Tian J M, Dai L X. Effects of drought and salt stress on osmotic and regulator and antioxidase activities[J]. Acta Abriculturae Boreali-Simica, 2018,33(3):176-181.
[20] 王永慧, 陈建平, 张培通, 高进, 周春霖, 施庆华, 周汝琴.盐胁迫对不同基因型黄秋葵苗期生长及生理生态特征的影响[J]. 华北农学报, 2016,31(6):105-110.doi:10.7668/hbnxb.2016.06.017.
Wng Y H, Chen J P, Zhang P T, Gao J, Zhou P T, Shi Q H, Zhou R Q. Effects of NaCl stress on growth and eco-physiological characteristics of different okra genotype in seedlings[J]. Acta Abriculturae Boreali-Simica, 2016,31(6):105-110.
[21] 刘延吉, 张珊珊, 田晓艳, 任大明. 盐胁迫对NHC牧草叶片保护酶系统、MDA含量及膜透性的影响[J]. 草原与草坪, 2008(2):30-34. doi:10.3969/j.issn.1009-5500.2008.02.007.
Lu Y J, Zhang S S, Tian X Y, Ren D M. Effects of salt stress on protective enzyme system,MDA content and membrane permeability of NHC foliar[J]. Grassland and Turf, 2008(2):30-34.
[22] 单长卷, 付远志, 彭贝贝.盐胁迫下谷胱甘肽对玉米幼苗根系抗氧化能力的影响[J].灌溉排水学报, 2015,34(10):56-59.doi:10.13522/j.cnki.ggps.2015.10.012.
San C J, Fu Z Y, Peng B B. Effects of glutathione on antioxidant properties of maize seedling roots under salt stress[J]. Journal of Irrigation and Drainage, 2015,34(10):56-59.
[23] Zhao F G, Sun C, Liu Y L, Liu Z P. Effects of salinity stress on the levels of covalently and noncovalently conjugated polyamines in plasma membrane and tonoplast isolated from barley seedlings[J]. Acta Botanica Sinica, 2000,42(9):920-926.
[24] 王素平, 李娟, 郭世荣, 胡晓辉, 李璟, 汪天.NaCl胁迫对黄瓜幼苗植株生长和光合特性的影响[J].西北植物学报, 2006,26(3):455-461.
Wng S P, Li J, Guo S R, Hu X H, Li J, Wang T. Effects of NaCl stress on growth and photosynthetic characteristics of cucumber (Cucumber sativus L.)seedlings[J].Acta Botanica Boreali-Occidentalia Sinica, 2006,26(3):455-461.
[25] 郑青松, 刘玲, 刘友良, 刘兆普. 盐分和水分胁迫对芦荟幼苗渗透调节和渗调物质积累的影响[J]. 植物生理与分子生物学学报,2003,29(6):585-588.doi:10.3321/j.issn:1671-3877.2003.06.017.
Zeng Q S, Liu L, Liu Y L, Liu Z P. Effects of salt and water stresses on osmotic adjustment and osmotica accumulation in Aloe vera seedlings[J]. Journal of Plant Physiology and Molecular Biology, 2003,29(6):585-588.
[26] 杨秀艳, 张华新, 张丽, 刘正祥, 杨升, 武香. NaCl胁迫对唐古特白刺幼苗生长及离子吸收、运输与分配的影响[J]. 林业科学, 2013, 49(9):165-171. doi:10.11707/j.1001-7488.20130924.
Yng X Y, Zhang H X, Zhang L, Liu Z X, Yang S, Wu X. Effects of NaCl stress on growth and absorption, transportation and distribution of ions in Nitraria tangutorum[J]. Scientia Silvae Sinicae, 2013, 49(9):165-171.
[27] 于玮玮, 曹波, 龙鸿, 李慧, 李爱, 阎国荣. 新疆野苹果幼苗对盐胁迫的生理响应[J]. 华北农学报, 2016,31(1):170-174.doi:10.7668/hbnxb.2016.01.027.
Y W W, Cao B, Long H, Li H, Li A, Yan G R. Physiological response of Malus ieversii seedling under sality stress[J]. Acta Agriculturae Boreali-Sinica,2016,31(1):170-174.

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