Genome-wide Identification and Functional Analysis of GlycoltransferaseⅠGene Family in Japonica Rice

doi:10.7668/hbnxb.20193138

Abstract

Abstract:

GlycosyltransferaseⅠ(GT Ⅰ)family is one of the glycosyltransferases,whose main functions involve the glycosyltransferase transfer of flavonoids and the synthesis of starch and sucrose.In order to reveal the role of GTⅠ gene family in the growth and development and high temperature tolerance of japonica rice, and to provide a basis for the functional study of GT Ⅰ gene family members in japonica rice,and provide ideas for rice variety improvement and high temperature tolerance. 30 members of GT Ⅰ gene family were identified by bioinformatics methods.The gene structure,physicochemical properties of proteins,chromosome location,gene evolution,phylogenetic relationship,conserved domain and protein three-dimensional structure modeling of members of GTⅠ genes in japonica rice were analyzed by bioinformatics.The results showed that 30 GT Ⅰ family members of japonica rice were distributed on 12 chromosomes of rice and the promoters of GT Ⅰ family members predicted abscisic acid response.The three-dimensional structure of family proteins was conserved and contained 6 β-folds and 7 α-helix.Transcriptome data and Real-time fluorescence quantitative analysis showed that most members of GT Ⅰ gene family responded to high temperature stress at 6 h after high temperature treatment.The expression level of OS-GT29 was continuously high.GT Ⅰ family was functionally similar in Arabidopsis thaliana and rice.

Key words: Japonica rice, Glycosyltransferase Ⅰ, Gene family, Bioinformatics, High temperature stress response

ZHOU Zhenzhong, LUO Biao, LUO Ying, XIA Min, WANG Qiming, RAO Liqun. Genome-wide Identification and Functional Analysis of GlycoltransferaseⅠGene Family in Japonica Rice[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 50-62. doi: 10.7668/hbnxb.20193138.

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References 51

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基因名 Gene name	基因ID Gene ID	正向引物(F)(5'—3') Forward primer(5'—3')	反向引物(R)(5'—3') Reverse primer(5'—3')
OS-GT01	Os04g0589600	TCCTCTCAATTAACCGGTTTGA	CCACCTGCTACTGTTAAGGTTA
OS-GT03	Os01g0262600	CCTCTCTTCCCCAATGGAC	GAGACGGCGATTAGTGGTTAG
OS-GT07	Os06g0229800	GAATTTGCAGAGGATAAGAGCG	CGATCACGTTCATGACATTCTC
OS-GT09	Os07g0412100	GGTAGATTTGCCAGAGAAGACT	GGACTAACCGTCATAACCAGAT
OS-GT11	Os01g0720600	ACTAGTTCTGGGTTTAATGGGG	CATTCCCACAAAAACTAACGGT
OS-GT14	Os11g0158400	ATTCATTTCCAGCAAGTGAACC	CTTTGCACGACACTAGCTATTC
OS-GT16	Os03g0268300	TTGATGTTCGAGTCTATCCTGG	ATTGTACATGTGGCAGTTAGGA
OS-GT19	Os03g0265100	AATGCCTTCAGAATCTGAGACA	CTCATTGCGTATTGTCTTCGAG
OS-GT22	Os11g0236100	GCTTCACTTCACTCTTGCAAAT	TATTAGTGACACGTCTGCTTGT
OS-GT29	Os03g0401300	TCAGCGTATCGAGGAGAAATAC	AATGCTCTCCTCTTTCAGAGTC

基因名 Gene name	基因ID Gene ID	正向引物(F)(5'—3') Forward primer(5'—3')	反向引物(R)(5'—3') Reverse primer(5'—3')
OS-GT01	Os04g0589600	TCCTCTCAATTAACCGGTTTGA	CCACCTGCTACTGTTAAGGTTA
OS-GT03	Os01g0262600	CCTCTCTTCCCCAATGGAC	GAGACGGCGATTAGTGGTTAG
OS-GT07	Os06g0229800	GAATTTGCAGAGGATAAGAGCG	CGATCACGTTCATGACATTCTC
OS-GT09	Os07g0412100	GGTAGATTTGCCAGAGAAGACT	GGACTAACCGTCATAACCAGAT
OS-GT11	Os01g0720600	ACTAGTTCTGGGTTTAATGGGG	CATTCCCACAAAAACTAACGGT
OS-GT14	Os11g0158400	ATTCATTTCCAGCAAGTGAACC	CTTTGCACGACACTAGCTATTC
OS-GT16	Os03g0268300	TTGATGTTCGAGTCTATCCTGG	ATTGTACATGTGGCAGTTAGGA
OS-GT19	Os03g0265100	AATGCCTTCAGAATCTGAGACA	CTCATTGCGTATTGTCTTCGAG
OS-GT22	Os11g0236100	GCTTCACTTCACTCTTGCAAAT	TATTAGTGACACGTCTGCTTGT
OS-GT29	Os03g0401300	TCAGCGTATCGAGGAGAAATAC	AATGCTCTCCTCTTTCAGAGTC

基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT01	Os04g0589600	418	46.58	7.73	质膜
OS-GT02	Os12g0583000	256	29.06	6.74	线粒体
OS-GT03	Os01g0262600	482	52.73	9.45	线粒体
OS-GT04	Os09g0296700	487	53.96	6.38	叶绿体
OS-GT05	Os02g0744700	677	74.15	6.48	叶绿体
OS-GT06	Os10g0437600	749	83.31	5.96	叶绿体
OS-GT07	Os06g0229800	810	88.37	5.14	叶绿体
OS-GT08	Os06g0160700	641	70.95	6.24	叶绿体
OS-GT09	Os07g0412100	608	67.35	6.69	叶绿体
OS-GT10	Os06g0133000	609	66.47	8.22	叶绿体
基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT11	Os01g0720600	975	109.89	5.92	胞质
OS-GT12	Os05g0533600	915	104.18	6.42	胞质
OS-GT13	Os01g0653200	503	55.85	8.48	线粒体
OS-GT14	Os11g0158400	736	82.01	7.25	叶绿体
OS-GT15	Os03g0214400	476	54.17	9.06	胞质
OS-GT16	Os03g0268300	463	53.30	8.27	胞质
OS-GT17	Os07g0100300	479	53.91	9.91	质膜
OS-GT18	Os01g0142300	436	49.15	7.16	胞质
OS-GT19	Os03g0265100	415	46.56	9.28	胞质
OS-GT20	Os07g0270950	471	53.08	7.19	质膜
OS-GT21	Os08g0301500	1 066	118.72	6.35	胞质
OS-GT22	Os11g0236100	509	53.89	7.19	叶绿体
OS-GT23	Os02g0184400	1 011	113.25	7.94	叶绿体
OS-GT24	Os04g0249500	844	96.47	8.09	胞质
OS-GT25	Os04g0309600	844	96.55	7.93	胞质
OS-GT26	Os02g0831500	846	96.15	6.35	胞质
OS-GT27	Os03g0340500	809	92.21	6.52	胞质
OS-GT28	Os07g0616800	816	93.11	6.59	胞质
OS-GT29	Os03g0401300	816	92.90	6.34	胞质
OS-GT30	Os06g0194900	808	92.12	6.35	胞质

基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT01	Os04g0589600	418	46.58	7.73	质膜
OS-GT02	Os12g0583000	256	29.06	6.74	线粒体
OS-GT03	Os01g0262600	482	52.73	9.45	线粒体
OS-GT04	Os09g0296700	487	53.96	6.38	叶绿体
OS-GT05	Os02g0744700	677	74.15	6.48	叶绿体
OS-GT06	Os10g0437600	749	83.31	5.96	叶绿体
OS-GT07	Os06g0229800	810	88.37	5.14	叶绿体
OS-GT08	Os06g0160700	641	70.95	6.24	叶绿体
OS-GT09	Os07g0412100	608	67.35	6.69	叶绿体
OS-GT10	Os06g0133000	609	66.47	8.22	叶绿体
基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT11	Os01g0720600	975	109.89	5.92	胞质
OS-GT12	Os05g0533600	915	104.18	6.42	胞质
OS-GT13	Os01g0653200	503	55.85	8.48	线粒体
OS-GT14	Os11g0158400	736	82.01	7.25	叶绿体
OS-GT15	Os03g0214400	476	54.17	9.06	胞质
OS-GT16	Os03g0268300	463	53.30	8.27	胞质
OS-GT17	Os07g0100300	479	53.91	9.91	质膜
OS-GT18	Os01g0142300	436	49.15	7.16	胞质
OS-GT19	Os03g0265100	415	46.56	9.28	胞质
OS-GT20	Os07g0270950	471	53.08	7.19	质膜
OS-GT21	Os08g0301500	1 066	118.72	6.35	胞质
OS-GT22	Os11g0236100	509	53.89	7.19	叶绿体
OS-GT23	Os02g0184400	1 011	113.25	7.94	叶绿体
OS-GT24	Os04g0249500	844	96.47	8.09	胞质
OS-GT25	Os04g0309600	844	96.55	7.93	胞质
OS-GT26	Os02g0831500	846	96.15	6.35	胞质
OS-GT27	Os03g0340500	809	92.21	6.52	胞质
OS-GT28	Os07g0616800	816	93.11	6.59	胞质
OS-GT29	Os03g0401300	816	92.90	6.34	胞质
OS-GT30	Os06g0194900	808	92.12	6.35	胞质

组 Group	基因ID组 Gene ID group	非同义突变率 Ka	同义突变率 Ks	核酸分子进化 Ka/Ks	P值 P-value(Fisher)
OS-GT29 vs OS-GT30	Os03g04013 & Os06g01949	0.980	1.067	0.919	0.003
OS-GT29 vs OS-GT25	Os03g04013 & Os04g03096	1.018	0.937	1.087	0.005
OS-GT29 vs OS-GT24	Os03g04013 & Os04g02495	1.019	0.936	1.088	0.005
OS-GT28 vs OS-GT30	Os07g06168 & Os06g01949	0.985	1.051	0.937	0.024
OS-GT28 vs OS-GT25	Os07g06168 & Os04g03096	1.017	0.943	1.078	0.011
OS-GT28 vs OS-GT24	Os07g06168 & Os04g02495	1.016	0.946	1.075	0.015

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