ACTA AGRICULTURAE BOREALI-SINICA ›› 2021, Vol. 36 ›› Issue (6): 1-6. doi: 10.7668/hbnxb.20192155

Special Issue: Radish Saline-alkali stress Biotechnology Hot Article

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Effect of Salt Stress on Seedling Growth and Transcription of RsCAT and RsSOD Genes in Radish

WANG Qingbiao1,2, WANG Yanping1,2, LINGHU Bo1,2, QIAN Huihui1,2, ZHAO Qiuju1, ZHANG Li1,2   

  1. 1. Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    2. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing 100097, China
  • Received:2021-09-21 Published:2021-12-28

Abstract: The effect of different concentrations salt stress on seedling growth and expression of related genes were explored in this study. Firstly, salt tolerant variety Yura Hama Daikon and susceptible variety Wujinhong were selected according to germination under salt stress conditions among 11 cultivars.And then the seedling height(SH) and the leaf scorch index(LSI) of Yura Hama Daikon and Wujinhong were investigated under salt stress treatment.The results showed that SH decreased and LSI increased under salt stress in both varieties.Compared with the salt-sensitive variety Wujinhong,the salt-tolerant variety Yura Hama Daikon had a smaller decrease in SH and lower LSI.Under 200 mmol/L salt stress,SH and LSI of salt-tolerant Yura Hama Daikon were 46.18% and 20.56,respectively,while those of salt-sensitive Wujinhong were 75.25% and 56.11.The transcription of RsCAT and RsSOD genes was studied in salt-tolerant and susceptible varieties under different salt concentrations by qPCR.The expression of RsCAT gene was first increased and then decreased under low salt concentration treatment,and reached the maximum at 7 day.When treated with high salt concentration,the transcription of RsCAT in Wujinhong was the highest at 48 h,while the expression level in salt-tolerant varieties increased gradually and maintained for a longer time,reaching the highest at 7 day.After high salt concentration treatment,the transcription of RsSOD gene reached the highest expression level at 24 hours,and then maintained a higher level in salt-tolerant variety.In salt-sensitive varieties,the maximum expression level of RsSOD appeared at 14 day.The above results will lay foundation for revealing the mechanism of salt stress in radish and provide technical support for radish salt-tolerant breeding.

Key words: Radish, Salt stress, RsCAT, RsSOD, Gene expression

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Cite this article

WANG Qingbiao, WANG Yanping, LINGHU Bo, QIAN Huihui, ZHAO Qiuju, ZHANG Li. Effect of Salt Stress on Seedling Growth and Transcription of RsCAT and RsSOD Genes in Radish[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 1-6. doi: 10.7668/hbnxb.20192155.

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