Screening of Salt-tolerant Cultivars of Brassica campestris L. and Its Salt-tolerant Mechanism

doi:10.7668/hbnxb.20194042

Abstract

Abstract:

The cultivation of salt-tolerant varieties is one of the measures to improve the yield reduction caused by secondary salinization. Therefore,the salt-tolerant varieties were screened and their tolerance mechanism were determined.Under control and salt treatment conditions,9 varieties of Brassica Campestris L. were used to compare the differences of salt-tolerance among varieties. Meanwhile,the salt-tolerant varieties and salt-sensitive varieties of Brassica Campestris L. were screened out. The photosynthetic characteristics,MDA content,relative conductivity,histochemical staining,SOD,POD,CAT,APX activity,root activity,nitrate content,and nitrate reductase activity of different varieties were determined under salt stress condition. Finally,the salt tolerance mechanism was preliminarily clarified.The growth indexes of 9 varieties were analyzed,and the salt-tolerant variety Lianzhou F₁ and salt-sensitive variety Jinqiuhong No.2 were selected. Compared with 15 mmol/L Ca(NO₃)₂ treatment (the control),the chlorophyll content,net photosynthetic rate,stomatal conductance,and transpiration rate of Lianzhou F₁ under 80 mmol/L Ca(NO₃)₂ stress were significantly lower than those of Jinqiuhong No.2. The relative conductivity,MDA content,O₂ $- ·$ ,and H₂O₂ content of Lianzhou F₁ had a slower degree of increasing than those of Jinqiuhong No.2 under Ca(NO₃)₂ stress,while the scavenging of oxygen free radicals had an opposite trend. Compared with the control,the increase of nitrate content in the shoot and root of Lianzhou F₁ under 80 mmol/L Ca(NO₃)₂ stress was lower than that of Jinqiuhong No.2,while the activity of nitrate reductase showed an opposite trend. Moreover,the root activity of Lianzhou F₁was less affected by nitrate than Jinqiuhong No.2.Lianzhou F₁ is a salt-tolerant Brassica Campestris L. variety,and Jinqiuhong No.2 is a salt-sensitive variety. Lianzhou F₁ has a low degree of oxidative stress,and a high photosynthetic efficiency and nitrate reducing ability,which can make it have a good growth ability under nitrate stress. Therefore,in the land with high degree of salinization,Lianzhou F₁ was recommended as the main cultivar.

Key words: Brassica Campestris L., Salt stress, Photosynthesis, Reactive oxygen scavenging, Nitrate reductase

ZHANG Ying, LI Haijun, GAO Fucheng, MU Xiaoguo, GAO Hu, WU Xue, YE Lin, CAO Kai. Screening of Salt-tolerant Cultivars of Brassica campestris L. and Its Salt-tolerant Mechanism[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 170-179. doi: 10.7668/hbnxb.20194042.

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Figures/Tables 8

Fig.1 Morphological comparison of nine Brassica campestris L. cultivars under Ca(NO₃)₂ stress Data are presented as means±s of three independent experiments. The different letters represents significant difference at P<0.05.The same as Fig.2—8.

Fig.2 Decline rate of plant height,root length,above ground dry fresh weight and lower ground dry fresh weight in Brassica campestris L. cultivars under Ca(NO₃)₂ stress

Fig.3 Chlorophyll content and photosynthetic characteristics of two Brassica campestris L.cultivars under Ca(NO₃)₂ stress

Fig.4 MDA contents and relative electric conductivity in leaves of two Brassica Campestris L. cultivars under Ca(NO₃)₂ stress

Fig.5

O 2 - ·

,H₂O₂ contents in leaves of two Brassica campestris L. cultivars under Ca(NO₃)₂ stress

Fig.5 $O 2 - ·$ ,H₂O₂ contents in leaves of two Brassica campestris L. cultivars under Ca(NO₃)₂ stress

Fig.6 SOD,CAT,POD and APX activity in leaves of two Brassica campestris L. cultivars under Ca(NO₃)₂ stress

Fig.7 Root activity of two Brassica campestris L. cultivars under Ca(NO₃)₂ stress

Fig.8 $\text{NO}_{3}^{-}$ contents and NR activity of two Brassica campestris L. cultivars under Ca(NO₃)₂ stress

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