Analysis of Salt Tolerance of ScALDH21 Transgenic Cotton

doi:10.7668/hbnxb.20193031

Abstract

Abstract:

In order to analyze the salt tolerance of ScALDH21 transgenic cotton at seedling stage,T₄ transgenic ScALDH21 cotton lines and its recipient Xinnongmian 1 was used as the research material,and the research were executed under field and greenhouse conditions.In field condition,the plant materials were planted with 0.4%,0.5% and 0.6% salt soil in stripe pots,respectively.The growth phenotype,the chlorophyll content and survival rate of each transgenic lines and its recipient under different salt concentrations were observed and monitored,and their net photosynthetic rate,stomatal conductance,transpiration rate and instantaneous water use efficiency were measured.In greenhouse condition,the contents of MDA,POD activity,lignin,Na⁺,K⁺ and Na⁺/K⁺ were determined after 150 mmol/L NaCl and water treatment respectively.Compared with the recipient cotton,the survival rate,chloroplast content,net photosynthetic rate,stomatal conductance,transpiration rate and instantaneous water use efficiency of ScALDH21 transgenic cotton were increased under salt stress.MDA content decreased,POD enzyme activity increased,lignin content increased.From the aspect of morphology and physiological indexes, it was verified that the transgenic ScALDH21 cotton improved the salt tolerance of the plant at the seedling stage.

Key words: Cotton, ALDH21 gene, Salt tolerance, Photosynthesis, Physiological indicators

CHENG Lihua, YANG Honglan, ZHANG Dawei, MA Qingqian, ZHANG Daoyuan. Analysis of Salt Tolerance of ScALDH21 Transgenic Cotton[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 1-7. doi: 10.7668/hbnxb.20193031.

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Figures/Tables 5

Fig.1 PCR detection of different transgenic cotton lines with ScALDH21 gene M.DNA standard molecular weight DL2000;1.Positive control;2.Negative control(779);3 and 4.Cotton lines 92 and 96 with ScALDH21 gene,respectively.

Fig.2 Phenotype,survival rate and relative content of chlorophyll of transgenic cotton with ScALDH21 gene under salt stress A.The growth state of recipient cotton and transgenic in different salt concentrations;B.The planting situation of strip basin in field;C.The survival rate of ScALDH21 transgenic cotton under different salt concentration;D.The relative content of chlorophyll in ScALDH21 transgenic cotton at different salt concentrations.779.Recipient cotton;Different letters represented significant difference between the transgenic line and the recipient cotton at the level of 0.05,P< 0.05.The same as Fig.3-5.

Fig.3 Photosynthetic parameters of ScALDH21 transgenic cotton at different salt concentrations

Fig.4 Changes of MDA content,POD activity and lignin content in transgenic cotton with ScALDH21 gene under salt stress

Fig.5 Changes of Na⁺,K⁺ and Na⁺/K⁺ of transgenic ScALDH21 cotton under salt stress 779.Salt treated non-transgenic plants;L92 and L96 were salt treated transgenic plants;CK.A non-transgenic plant without salt treatment.

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