Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (2): 18-27. doi: 10.7668/hbnxb.20192703

Special Issue: Wheat Biotechnology

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Bioinformatics and Expression Pattern Analysis of Senescence-associated Gene TaSAG39 in Wheat

SUN Lili1,2, JIN Xiujuan1,2, ZHAO Kai1,2, Md Ashraful Islam1,2, LU Juan1,2, WANG Shuguang1,2, SUN Daizhen1,2   

  1. 1.College of Agriculture,Shanxi Agricultural University,Taigu 030801,China
    2.The Provincial and Ministerial Cooperative National Key Laboratory of Organic Dry Farming(in Preparation),Taiyuan 030031,China
  • Received:2022-01-23 Published:2022-04-28


Cysteine protease,a kind of hydrolase in the process of protein degradation,participates in the senescence and maturation of plants and plays an important role in the growth and development of plants.In this study,a senesence-specific cysteine protease(SAG39)was screened from the previously obtained transcriptome data of wheat at senescence stage.In order to study the role of TaSAG39 gene in wheat at senescence,the gene structure and expression pattern of TaSAG39 were analyzed by bioinformatics analysis and Real-time fluorescence quantitative(qRT-PCR)technology.The results showed that the amino acid sequence encoded by the cysteine protease gene SAG39 had the closest relationship with the ancestral species of wheat,such as Aegilops tauschii,Triticum dicoccoides and Triticum durum,and it included the unique active site Cys-His-Asn and EFNIN structures of the papain sub-family.The full-length genomes of TaSAG39-5A,TaSAG39-5B and TaSAG39-5D were 1 455,1 435,1 439 bp,respectively,and the length of the coding sequence was 1 041,1 050,1 038 bp,respectively,with each containing two exons and one intron.The TaSAG39s proteins consisted of 346,349,345 amino acids.With relative molecular weight of about 37 ku and isoelectric point of 5.53—5.67,these proteins were stable negatively charged hydrophilic proteins.Its main components were random coils and α-helix,and there were signal peptides in the N segment of the protein.It had the conserved Inhibittor_I29 and Petidase_C1 domains,and contained 35—37 phosphorylation sites,mainly serine and threonine phosphorylation sites.TaSAG39 protein may interact with cysteine protease inhibitors and 14-3-3 protein.The promoter contained cis-acting elements relative to abscisic acid,methyl jasmonate,auxin,light,drought,low temperature and stress response.The results of qRT-PCR confirmed that gene TaSAG39 was induced to express by natural aging,darkness,drought,methyl jasmonate,high temperature and auxin,among which natural aging was the most significant.

Key words: Wheat, TaSAG39, Cysteine protease, Bioinformatics analysis, Expression pattern

Cite this article

SUN Lili, JIN Xiujuan, ZHAO Kai, Md Ashraful Islam, LU Juan, WANG Shuguang, SUN Daizhen. Bioinformatics and Expression Pattern Analysis of Senescence-associated Gene TaSAG39 in Wheat[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 18-27. doi: 10.7668/hbnxb.20192703.

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