Relationship Between Stem Microstructure,Biochemical Composition and Stem Strength of Different Wheat Varieties(Lines)

doi:10.7668/hbnxb.20192493

Abstract

Abstract:

In order to explore the key factors affecting stem strength and screen the valuable parent resources for wheat "strong stem breeding" ,72 wheat varieties(lines)were used as materials.Firstly,the strength of stems in flowering stage,filling stage and milk stage of wheat was comprehensively analyzed and all the materials were grouped into clusters.On this basis,the genetic variation of lignin content,cellulose content and microstructure of wheat stem and their relation with stem strength were further analyzed,and the key characters related to stem strength were defined.In addition,principal component analysis(PCA)was used to extract the main factors of stem cross section microstructure,and calculate the score of comprehensive factors,and further cluster analysis was carried out combined with lignin and cellulose content,so as to comprehensively evaluate the performance of stem strength related traits of different wheat varieties(lines).The results showed that the mechanical tissue thickness decreased significantly or extremely significant at the same time as the strength of wheat stem weakened from flowering stage to filling stage.Moreover,parenchyma thickness,mechanical tissue thickness,large vascular bundle area,lignin and cellulose content in high stem strength wheat were much higher than those in low stem strength wheat.Correlation analysis also showed that mechanical tissue thickness,large vascular bundle area,lignin and cellulose content were significantly of extremely significantly correlated with stem strength.Finally,the lignin content,cellulose content and the main component comprehensive factor scores of the stem microstructure were used as three variables for cluster analysis,and a total of 18 wheat materials with outstanding performance in the stem microstructure and biochemical indicators were screened out.These wheat materials can be used as important genetic resources for wheat "strong stem breeding".

Key words: Wheat, Strong stem breeding, Stem strength, Microstructure, Lignin content, Cellulose content

HE Jie, SUN Shaoguang, GE Changbin, SONG Danyang, QIAO Jiliang, LI Suoping, SU Yarui, LIAO Pingan. Relationship Between Stem Microstructure,Biochemical Composition and Stem Strength of Different Wheat Varieties(Lines)[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 68-76. doi: 10.7668/hbnxb.20192493.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192493

http://www.hbnxb.net/EN/Y2022/V37/I1/68

Figures/Tables 7

References 29

[1]	武翠卿,孙静鑫,郭平毅,王宏富,武新慧.农艺措施对谷子产量及抗倒伏力学性能的影响[J].中国农业科学,2021,54(6):1127-1142.doi:10.3864/j.issn.0578-1752.2021.06.005.
	Wu C Q,Sun J X,Guo P Y,Wang H F,Wu X H.Effects of agronomic managements on yield and lodging resistance of millet[J].Scientia Agricultura Sinica,2021, 54(6):1127-1142.
[2]	姚佳延,于国琦,洪益,吕超,许如根.大麦株高类性状的遗传分析与QTL定位[J].华北农学报,2021,36(2):28-32.doi:10.7668/hbnxb.20191631.
	Yao J Y,Yu G Q,Hong Y,Lü C,Xu R G.Genetic analysis and QTL mapping of plant height-related characters in barley[J].Acta Agriculturae Boreali-Sinica,2021,36(2):28-32.
[3]	Zhang J,Li G H,Song Y P,Liu Z G,Yang C D,Tang S,Zheng C Y,Wang S H,Ding Y F.Lodging resistance characteristics of high-yielding rice populations[J].Field Crops Research,2014,161:64-74.doi:10.1016/j.fcr.2014.01.012.
[4]	胡卫国,曹廷杰,杨剑,张玉娥,王西成,曹颖妮,赵虹.小麦新品种(系)抗倒性及产量构成因素评价[J].种子,2021,40(2):110-115.doi:10.16590/j.cnki.1001-4705.2021.02.110.
	Hu W G,Cao T J,Yang J,Zhang Y E,Wang X C,Cao Y N,Zhao H.Evalation of lodging resistance and yield components of new wheat varieties(lines)[J].Seed,2021,40(2):110-115.
[5]	肖世和,张秀英,闫长生,张文祥,海林,郭会君.小麦茎秆强度的鉴定方法研究[J].中国农业科学,2002,35(1):7-11.doi:10.3321/j.issn:0578-1752.2002.01.002.
	Xiao S H,Zhang X Y,Yan C S,Zhang W X,Hai L,Guo H J.Determination of resistance to lodging by stem strength in wheat[J].Scientia Agricultura Sinica,2002,35(1):7-11.doi:10.3321/j.issn:0578-1752.2002.01.002.
[6]	Miller C N,Harper A L,Trick M,Werner P,Waldron K,Bancroft I.Elucidation of the genetic basis of variation for stem strength characteristics in bread wheat by associative transcriptomics[J].BMC Genomics,2016,17(1):500.doi:10.1186/s12864-016-2775-2.
[7]	Kashiwagi T,Togawa E,Hirotsu N,Ishimaru K.Improvement of lodging resistance with QTLs for stem diameter in rice(Oryza sativa L.)[J].Theoretical and Applied Genetics,2008,117(5):749-757.doi:10.1007/s00122-008-0816-1.
[8]	Kelbert A J,Spaner D,Briggs K G,King J R.The association of culm anatomy with lodging susceptibility in modern spring wheat genotypes[J].Euphytica,2004,136(2):211-221.doi:10.1023/B:EUPH.0000030670.36730.a4.
[9]	Dunn G J,Briggs K G.Variation in culm anatomy among barley cultivars differing in lodging resistance[J].Canadian Journal of Botany,1989,67(6):1838-1843.doi:10.1139/b89-232.
[10]	Wang J,Zhu J M,Lin Q Q,Li X J,Teng N J,Li Z S,Li B,Zhang A M,Lin J X.Effects of stem structure and cell wall components on bending strength in wheat[J].Chinese Science Bulletin,2006,51(7):815-823.doi:10.1007/s11434-006-0815-z.
[11]	Muhammad A,Hao H H,Xue Y L,Alam A,Bai S M,Hu W C,Sajid M,Hu Z,Samad R A,Li Z H,Liu P Y,Gong Z Q,Wang L Q.Survey of wheat straw stem characteristics for enhanced resistance to lodging[J].Cellulose,2020,27(5):2469-2484.doi:10.1007/s10570-020-02972-7.
[12]	Spatz H,Köhler L,Speck T.Biomechanics and functional anatomy of hollow-stemmed sphenopsids.I.Equisetum giganteum(Equisetaceae)[J].American Journal of Botany,1998,85(3):305.
[13]	Shah A N,Tanveer M,Rehman A U,Anjum S A,Iqbal J,Ahmad R.Lodging stress in cereal-effects and management:An overview[J].Environmental Science and Pollution Research International,2017,24(6):5222-5237.doi:10.1007/s11356-016-8237-1.
[14]	杨霞,王红娟,徐文静,陈军营.不同抗倒性小麦品种的茎秆结构及其化学成分和力学特性分析[J].河南农业大学学报,2012,46(4):370-373.doi:10.3969/j.issn.1000-2340.2012.04.004.
	Yang X,Wang H J,Xu W J,Chen J Y.Analysis of microstructure of stem and its chemical composition and mechanical property in different lodging resistance wheat cultivars[J].Journal of Henan Agricultural University,2012,46(4):370-373.
[15]	陈晓光,史春余,尹燕枰,王振林,石玉华,彭佃亮,倪英丽,蔡铁.小麦茎秆木质素代谢及其与抗倒性的关系[J].作物学报,2011,37(9):1616-1622.doi:10.3724/SP.J.1006.2011.01616.
	Chen X G,Shi C Y,Yin Y P,Wang Z L,Shi Y H,Peng D L,Ni Y L,Cai T.Relationship between lignin metabolism and lodging resistance in wheat[J].Acta Agronomica Sinica,2011,37(9):1616-1622.
[16]	韩新峰.232份小麦品种资源抗倒伏相关因素分析[D].合肥:安徽农业大学,2014.
	Han X F.Study on lodging resistance in 232 wheat varieties resources[D].Hefei:Anhui Agricultural University,2014.
[17]	Pi$\bar{n}$era-Chavez F J,Berry P M,Foulkes M J,Reynolds M A,Reynolds M P.Avoiding lodging in irrigated spring wheat.I.Stem and root structural requirements[J]. Field Crops Research,2016,196:325-336.doi:10.1016/j.fcr.2016.06.009.
[18]	胡卫国,曹廷杰,王西成,张玉娥,常萍,赵虹.小麦品种抗倒伏能力定性定量研究[J].华北农学报,2018,33(5):160-167.doi:10.7668/hbnxb.2018.05.022.
	Hu W G,Cao T J,Wang X C,Zhang Y E,Chang P,Zhao H.Qualitative and quantitative study on lodging resistance of wheat varieties[J].Acta Agriculturae Boreali-Sinica,2018,33(5):160-167.
[19]	董秀春,韩伟,杨洪宾.倒伏对冬小麦病害发生情况和产量的影响[J].河南农业科学,2016,45(4):27-30.doi:10.15933/j.cnki.1004-3268.2016.04.006.
	Dong X C,Han W,Yang H B.Effects of lodging on winter wheat diseases occurrence and yield[J].Journal of Henan Agricultural Sciences,2016,45(4):27-30.
[20]	Charlotte M.The genetic control of stem mechanical properties in Brassica napus and wheat[D].Norwich:University of East Anglia,2014.
[21]	Yao J B, Ma H X, Zhang P P, Ren L J, Yang X M, Yao G C, Peng Z, Zhou M P.Inheritance of stem strength and its correlations with culm morphological traits in wheat(Triticum aestivum L.)[J].Canadian Journal of Plant Science,2011,91(6):1065-1070.doi:10.4141/cjps2011-033.
[22]	Kong E, Liu D C, Guo X L, Yang W L, Sun J Z, Li X, Zhan K H,Cui D Q,Lin J X,Zhang A M.Anatomical and chemical characteristics associated with lodging resistance in wheat[J].The Crop Journal,2013,1(1):43-49.doi:10.1016/j.cj.2013.07.012.
[23]	Kaack K,Schwarz K U,Brander P E.Variation in morphology,anatomy and chemistry of stems of Miscanthus genotypes differing in mechanical properties[J].Industrial Crops and Products,2003,17(2):131-142.doi:10.1016/S0926-6690(02)00093-6.
[24]	南铭.燕麦抗倒伏生理机制及茎秆木质素合成基因表达研究[D].兰州:甘肃农业大学,2021.doi:10.27025/d.cnki.ggsnu.2021.000011.
	Nan M.Study expression of genes in stem lignin synthesis and physiological mechanism of lodging resistance in oat[D].Lanzhou:Gansu Agricultural University,2021.
[25]	Wu L M,Zhang W J,Ding Y F,Zhang J W,Cambula E D,Weng F,Liu Z H,Ding C Q,Tang S,Chen L,Wang S H,Li G H.Shading contributes to the reduction of stem mechanical strength by decreasing cell wall synthesis in japonica rice(Oryza sativa L.)[J].Frontiers in Plant Science,2017,8:881.doi:10.3389/fpls.2017.00881.
[26]	Liu W G,Hussain S,Liu T,Zou J L,Ren M L,Zhou T,Liu J,Yang F,Yang W Y.Shade stress decreases stem strength of soybean through restraining lignin biosynthesis[J].Journal of Integrative Agriculture,2019,18(1):43-53.doi:10.1016/S2095-3119(18)61905-7.
[27]	范文秀,侯玉霞,冯素伟,朱芳坤,茹振钢.小麦茎秆抗倒伏性能研究[J].河南农业科学,2012,41(9):31-34.doi:10.15933/j.cnki.1004-3268.2012.09.021.
	Fan W X,Hou Y X,Feng S W,Zhu F K,Ru Z G.Study on cellulose and lodging resistance of wheat straw[J].Journal of Henan Agricultural Sciences,2012,41(9):31-34.
[28]	Zhang W J, Wu L M, Ding Y F,Weng F, Wu X R, Li G H, Liu Z H, Tang S, Ding C Q, Wang S H.Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice[J].Journal of Integrative Agriculture,2016, 15(5):992-1004. doi:10.1016/S2095-3119(15)61166-2.
[29]	Peng D L, Chen X G, Yin Y P, Lu K L, Yang W B, Tang Y H, Wang Z L.Lodging resistance of winter wheat(Triticum aestivum L.):Lignin accumulation and its related enzymes activities due to the application of paclobutrazol or gibberellin acid[J].Field Crops Research,2014,157:1-7.doi:10.1016/j.fcr.2013.11.015.

性状 Traits	开花期 Flowering stage		灌浆期 Filling stage
性状 Traits	均值±标准差 Mean±s	变异系数/% CV	均值±标准差 Mean±s	变异系数/% CV
茎秆强度/g SS	2 102.62±632.48a	30.08	1 741.99±583.52b	33.50
薄壁组织厚度/μm TP	1 181.12±160.63a	13.60	1 125.40±256.53a	22.80
机械组织厚度/μm TMT	97.35±12.93a	13.28	84.90±19.36b	22.80
大维管束面积/(×10³μm²)TALVB	912.35±171.55a	18.80	813.57±150.72a	18.53
大维管束数目/个 NLVB	43.00±4.66a	10.84	45.51±5.31a	11.67
小维管束数目/个 NSVB	17.38±5.40a	31.07	18.21±6.05a	33.23
机械组织层数/层 NLMT	6.63±0.35a	5.34	6.42±0.70a	10.87
小维管束比例/% PSVB	0.25±0.09a	35.70	0.28±0.07a	25.33

性状 Traits	开花期 Flowering stage		灌浆期 Filling stage
性状 Traits	均值±标准差 Mean±s	变异系数/% CV	均值±标准差 Mean±s	变异系数/% CV
茎秆强度/g SS	2 102.62±632.48a	30.08	1 741.99±583.52b	33.50
薄壁组织厚度/μm TP	1 181.12±160.63a	13.60	1 125.40±256.53a	22.80
机械组织厚度/μm TMT	97.35±12.93a	13.28	84.90±19.36b	22.80
大维管束面积/(×10³μm²)TALVB	912.35±171.55a	18.80	813.57±150.72a	18.53
大维管束数目/个 NLVB	43.00±4.66a	10.84	45.51±5.31a	11.67
小维管束数目/个 NSVB	17.38±5.40a	31.07	18.21±6.05a	33.23
机械组织层数/层 NLMT	6.63±0.35a	5.34	6.42±0.70a	10.87
小维管束比例/% PSVB	0.25±0.09a	35.70	0.28±0.07a	25.33

性状 Traits	高强度 High strength	低强度 Low strength
薄壁组织厚度/μm TP	1 299.80±253.60Aa	1 020.39±178.53Bb
机械组织厚度/μm TMT	81.69±19.04Aa	64.29±14.83Ab
大维管束面积/(×10³ μm²)TALVB	906.63±188.21Aa	746.45±974.40Ab
大维管束数目/个 NLVB	46.27±4.05Aa	43.17±5.62Aa
小维管束数目/个 NSVB	17.45±5.00Aa	19.42±7.19Aa
机械组织层数/层 NLMT	6.27±0.68Aa	6.17±0.78Aa
小维管束比例/% PSVB	0.27±0.06Aa	0.30±0.07Aa

性状 Traits	高强度 High strength	低强度 Low strength
薄壁组织厚度/μm TP	1 299.80±253.60Aa	1 020.39±178.53Bb
机械组织厚度/μm TMT	81.69±19.04Aa	64.29±14.83Ab
大维管束面积/(×10³ μm²)TALVB	906.63±188.21Aa	746.45±974.40Ab
大维管束数目/个 NLVB	46.27±4.05Aa	43.17±5.62Aa
小维管束数目/个 NSVB	17.45±5.00Aa	19.42±7.19Aa
机械组织层数/层 NLMT	6.27±0.68Aa	6.17±0.78Aa
小维管束比例/% PSVB	0.27±0.06Aa	0.30±0.07Aa

性状 Traits	茎秆强度 SS	薄壁组织厚度 TP	机械组织厚度 TMT	大维管束面积 TALVB	大维管束数目 NLVB	小维管束数目 NSVB	机械组织层数 NLMT	小维管束比例 PSVB
茎秆强度SS		0.601	0.831^**	0.397^**	0.175	0.267	0.114	0.307^*
薄壁组织厚度TP	0.129		0.332	0.074	0.131	0.202	-0.301	0.225
机械组织厚度TMT	0.359^**	0.101		-0.147	-0.236	0.228	-0.057	0.213
大维管束面积TALVB	0.339^**	0.169	0.290		0.543^**	-0.086	-0.252	-0.112
大维管束数目NLVB	0.050	0.016	0.034	0.510^**		-0.212	0.073	-0.253
小维管束数目NSVB	0.053	-0.106	0.264	-0.040	0.039		-0.564^**	0.984^**
机械组织层数NLMT	0.177	0.010	0.457^**	0.281	0.175	0.050		-0.224
小维管束比例PSVB	0.054	-0.095	0.226	-0.188	-0.295^*	0.933^**	0.012

Please choose a citation manager

Content to export