Detection of Wheat Leaf Rust Resistance Gene Lr21 and Resistance Evaluation to Leaf Rust and Powdery Mildew

doi:10.7668/hbnxb.20195867

Abstract

Abstract:

Wheat leaf rust and wheat powdery mildew are two important diseases in the world.Cultivating and planting disease resistant varieties is the most economical and effective method to control these two diseases.In order to test the distribution of wheat leaf rust resistance gene Lr21,the molecular marker closely linked to Lr21 was used to detect 1 200 wheat varieties(lines),and 23 wheat varieties(lines)were detected to contain Lr21.The molecular markers related to other leaf rust resistance genes were used to detect 23 wheat materials,Lr20 gene was detected in Gaoyou 2018,Lr37 gene was detected in Aifeng 8,Jingdong 22,Zhongmai 175,Luyuan 205 and Tang Y958,and Lr46 gene was detected in Jinan 17.No wheat varietie(line)was detected to contain Lr9,Lr10,Lr19,Lr24 or Lr34.Resistance evaluation of 23 wheat varieties(lines)containing Lr21 was identified using the epidemic races of Puccinia triticina(Pt)and Blumeria graminis f.sp.tritici(Bgt),respectively.The results showed that seven wheat varieties(lines)were resistant to both Pt and Bgt,with a frequency of 30.43%,and six wheat varieties(lines)were susceptible to Pt and Bgt mildew at the same time,and the frequency was 26.09%.Taken together,Lr21 was detected in 1 200 wheat cultivars(lines),as well as the presence of other rust resistance genes,and further evaluated their resistance to Pt and Bgt,which will provide a theoretical basis for the selection of multi-resistant wheat.

Key words: Wheat leaf rust, Wheat powdery mildew, Resistance gene, Molecular detection

CLC Number:

LIU Yuanxia, SONG Maoxing, DING Dashuo, JIN Yuqing, LIU Changshan, WANG Haiyan, WU Zhihui. Detection of Wheat Leaf Rust Resistance Gene Lr21 and Resistance Evaluation to Leaf Rust and Powdery Mildew[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 240-247. doi: 10.7668/hbnxb.20195867.

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Figures/Tables 5

Tab.1 Primers used in this study

基因 Gene	标记引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	退火温度/℃ Annealing temperature
Lr9	J13/1	TCCTTTTATTCCGCACGCCGG	68.5
	J13/2	CCACACTACCCCAAAGAGACG
Lr10	Fl.2245	GTGTAATGCATGCAGGTTCC	60.0
	Lr10-6/r2	AGGTGTGAGTGAGTTATGTT
Lr19⁽⁺⁾	SCS265-F	GGCGGATAAGCAGAGCAGAG	65.0
	SCS265-R	GGCGGATAAGTGGGTTATGG
Lr19^(-)	SCS253-F	GCTGGTTCCACAAAGCAAA	60.0
	SCS253-R	GGCTGGTTCCTTAGATAGGTG
Lr20	STS638-L	ACAGCGATGAAGCAATGAAA	60.0
	STS638-R	GTCCAGTTGGTTGATGGAAT
Lr21	D14-L	CGCTTTTACCGAGATTGGTC	65.0
	D14-R	TCTGGTATCTCACGAAGCCTT
Lr24	J09/1	TCTAGTCTGTACATGGGGGC	60.0
	J09/2	TGGCACATGAACTCCATACG
Lr34	csLv34F	GTTGGTTAAGACTGGTGATGG	55.0
	csLv34R	TGCTTGCTATTGCTGAATAGT
Lr37	VENTRIUP	AGGGGCTACTGACCAAGGCT	65.0
	LN2	TGCAGCTACAGCAGTATGTACACAAAA
Lr46	cs LV46G22-F	TCGACTTTGGAATGGAGTTGC	60.0
	cs LV46G22-R	GGCGAAGATGCCATCATCCACCAG

Fig.1 Mixed pool detection results of wheat leaf rust resistance gene Lr21 in 1 200 wheat cultivars (lines) M.DL2000 DNA Marker;A.TcLr21;B.Thatcher;1-120.PCR detection of 120 DNA mixed pool.

Fig.2 Detection results of leaf rust resistance gene Lr21 in some wheat cultivars (lines) M.DL2000 DNA Marker;A.TcLr21;B.Thatcher;1.Aifeng 8;2.Hongmai 15-1;3.Lunxuan 103;4.Tang Y2;5.Huamai 007;6.Jinhuafeng 1;7.Fengchuanjiu;8.Jing 9428;9.Huamai 158;10.Shijiazhuang 11;11.Zhengmai 136;12.Huamai 9518;13.Zhongxinmai 48;14.Jimai 23;15.Liangxing 77;16.Xinmai 807;17.Yudancheng 339;18.Hengmai 30;19.Liangxing 99;20.Jingdong 20;21.Jimai 765;22.Hannong 1412;23.Jingdong 22;24.Jimai 22;25.Lunxuan 145;26.Gaoyou 2018;27.Jimai 38;28.Ketengmai 16;29.Shixin 633;30.Longtang 2;31.Jingdong 23;32.Hemai 30;33.Shixin 811;34.Tiancimai 1;35.Wanfeng 505;36.Zhongmai 175;37.Malan 6;38.Malan 2;39.Malan 1;40.Jingdong 11;41.Jimai 55;42.Nongda 399;43.Ningtai 833;44.Heng 4399;45.Yannong 1212;46.Lande 856;47.Womai 33;48.Shannong 42;49.Zhongxinmai 998;50.Yannong 999;51.Baofeng 104;52.Lunxuan 987;53.Jinzuan 9;54.Shinong 958;55.Shannong 28;56.Jingdong 18;57.Jingdong 12;58.Lunxuan 103;59.Guan 35;60.Luyuan 128;61.Hanmai 25;62.Lunxuan 45;63.Luyuan 502;64.Jimai U80;65.Jimai U87;66.Jimai 479;67.Jimai 665;68.Luyuan 205;69.Jimai 713;70.Jizimai 3;71.Jizimai 439;72.Tang Y605;73.Cangmai 6002;74.Cangmai 6005;75.Jiemai 19;76.Nongda 135;77.Jimai 21;78.Xinmai 296;79.Jinan 17;80.Shimai 22;81.Tang Y958, 82.Zhengyou 6;83.Xinong 857;84.Tang Y959;85.Qimin 21;86.Xindong 20;87.Kenong 2011;88.Shannong 1355;89.Liangxing 66;90.Lunxuan 310.

Fig.3 Detection results of other leaf rust resistance gene in 23 wheat cultivars (lines) A.Molecular Marker detection of Lr9 gene;B.Molecular Marker detection of Lr10 gene;C.Molecular Marker detection of Lr19⁽⁺⁾ gene;D.Molecular Marker detection of Lr19^(-) gene;E.Molecular Marker detection of Lr24 gene;F.Molecular marker detection of Lr34 gene;G.Molecular Marker detection of Lr20 gene;H.Molecular Marker detection of Lr37 gene;I.Molecular Marker detection of Lr46 gene.M.DL2000 DNA Marker;A1-A9.TcLr9,TcLr10,TcLr19,TcLr19,TcLr20,TcLr24,TcLr34,TcLr37,TcLr46;B.Thatcher;1.Hongmai 15-1;2.Aifeng 8;3.Tang Y2;4.Jing 9428;5.Gaoyou 2018;6.Shijiazhuang11;7.Jingdong 22;8.Jingdong 20;9.Jingdong 23;10.Zhongmai 175;11.Jingdong 11;12.Heng 4399;13.Baofeng 104;14.Lunxuan 987;15.Jingdong 18;16.Jingdong 12;17.Luyuan 502;18.Luyuan 205;19.Tang Y605;20.Jimai 21;21.Jinan 17;22.Tang Y958;23.Tang Y959.

Tab.2 Identification of wheat resistant to leaf rust and powdery mildew

小麦品种 Wheat cultivars	小麦叶锈病 Wheat leaf rust		小麦白粉病 Wheat powdery mildew
小麦品种 Wheat cultivars	反应型 Reaction type	抗性评价 Evaluation of resistance	反应型 Reaction type	抗性评价 Evaluation of resistance
矮丰8号 Aifeng 8	3	中感	1	高抗
弘麦15-1 Hongmai 15-1	1	高抗	3	中感
唐Y2 Tang Y2	2	中抗	3	中感
京9428 Jing 9428	3	中感	2	中抗
石家庄11号 Shijiazhuang 11	2	中抗	3	中感
京冬20 Jingdong 20	4	高感	4	高感
京冬22 Jingdong 22	2	中抗	4	高感
藁优2018 Gaoyou 2018	1	高抗	3	中感
京冬23 Jingdong 23	2	中抗	3	中感
中麦175 Zhongmai 175	3	中感	4	高感
京冬11 Jingdong 11	2	中抗	2	中抗
衡4399 Heng 4399	1	高抗	1	高抗
保丰104 Baofeng 104	1	高抗	1	高抗
轮选987 Lunxuan 987	1	高抗	2	中抗
京冬18 Jingdong 18	4	高感	4	高感
京冬12 Jingdong 12	4	高感	4	高感
鲁原502 Luyuan 502	2	中抗	2	中抗
鲁原205 Luyuan 205	2	中抗	3	中感
唐Y605 Tang Y605	2	中抗	2	中抗
济麦21 Jimai 21	3	中感	3	中感
济南17 Jinan 17	3	中感	3	中感
唐Y958 Tang Y958	2	中抗	2	中抗
唐Y959 Tang Y959	2	中抗	3	中感

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