Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus in Tianjin Based on Small RNA Sequencing

doi:10.7668/hbnxb.20192006

Abstract

Abstract: In order to clarify the cause of tomato fruit browning in Tianjin, deep sequencing of small RNA and RT-PCR were used.The results showed that this symptom was caused by Tomato mosaic virus (ToMV). The sequences of CP(Coat Protein, CP), MP(Movement Protein, MP), and 126 proteins of the virus were further analyzed. Phylogenetic analysis showed that the three genes of the Tianjin isolate(ToMV-TJ) were the closest to the American isolate(USA_99-1).The similarity analysis showed that the average similarity of ToMV-TJ CP gene to other CP gene isolates was 95.53%.The average similarity of MP gene to other MP gene isolates was 95.21%.The average similarity of 126 protein gene to other 126 protein gene isolates was 92.72%.The proteins encoded by these three genes were analyzed. The results showed that the average similarity was above 99.0%. It is initially shown that ToMV-TJ isolate was imported through international trade.

Key words: Tomato, Tomato mosaic virus, Small RNA deep sequencing, Molecular detection, Gene sequence analysis

CLC Number:

S436.412.1+1

JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus in Tianjin Based on Small RNA Sequencing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183. doi: 10.7668/hbnxb.20192006.

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