Isolation, Identification, Drug Sensitivity Test and Detection of Drug Resistance Genes of Bovine Acinetobacter calcoaceticus

doi:10.7668/hbnxb.201751717

Abstract

Abstract: In order to study the pathogenic mechanism and prevention and control measures of Acinetobacter calcoaceticus, the pathogen isolation of the diseased dead bovine lung tissue suspected of having Acinetobacter calcoaceticus was obtained, and one dominant bacteria was named 9-1. The isolates were subjected to morphological observation, biochemical test, 16S rDNA gene sequence determination, animal pathogenicity test and drug susceptibility test. The results showed that the isolated strain was Gram-negative bacillus and had strong pathogenicity to the test mice. It was identified as Acinetobacter calcoaceticus by biochemical test and 16S rDNA gene sequence analysis. The drug susceptibility test showed that the isolated strain was resistant to florfenicol, cefotaxime, cephalexin, and azithromycin, and was sensitive to the other eight test drugs. The results of β-lactam (TEM, DHA, OXA-23, OXA-58) drug resistance genes were all negative. This test provides a scientific basis for the selection of reasonable antibacterial drugs for diseases caused by Acinetobacter calcoaceticus, and lays a foundation for further research on its resistance mechanism.

Key words: Acinetobacter calcoaceticus, Isolation and identification, Drug sensitive test, Resistance gene

CLC Number:

S855.1

LI Chen, WANG Siyue, JI Shaoze, LI Xuesong, ZHANG Xiqing, TIAN Jiaqi, MA Hongxia, GAO Yunhang. Isolation, Identification, Drug Sensitivity Test and Detection of Drug Resistance Genes of Bovine Acinetobacter calcoaceticus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 318-323. doi: 10.7668/hbnxb.201751717.

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http://www.hbnxb.net/EN/Y2019/V34/IS1/318

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