Identification and Analysis of Resistance Genes to Bacterial Blight in Yuanjiang Common Wild Rice

doi:10.7668/hbnxb.20193594

Abstract

Abstract:

Yuanjiang common wild rice(YP)is a germplasm material known as a living fossil in plants,which also has a large number of resistance(R)genes.Therefore,knowing the R genes in YP will help to apply different R genes to cultivated rice.Here,we identified the bacterial blight(BB)R genes in Yuanjiang common wild rice(YP),progeny of introgression lines(L214 and G252)and its susceptible parent,cultivated rice line 35(HX35).Meanwhile,the resistance of YP and its introgression lines to several Xanthomonas oryzae pv.oryzae (Xoo)strains was determined at the late tillering stage of rice.Under indoor control conditions,YP and its introgression lines showed resistance to 9 different Xoo strains(C1,C2,C3,C4,C5,C6,C7,C9 and PXO99^A),indicating that they had broad-spectrum resistance to BB.The results of bacterial blight resistance gene detection showed that YP contained homologous genes of Xa10,while HX35,L214 and G252 contained susceptible homologous genes of xa23.The sequence alignment results showed that the sequence of the effector binding element (EBE_AvrXa10) region of the disease resistance gene Xa10 was significantly different from that of the Xa10 promoter in YP. Similarly, the recessive susceptible gene xa23 was consistent with the effector binding element (EBE_AvrXa23) region of the xa23 promoter in HX35, L214 and G252. The qRT-PCR results showed that the immune responses in YP,HX35,L214 and G252 were activated after inoculation with strain PXO99^A,but Xa10 in YP and xa23 in HX35,L214 and G252 were not induced to be expressed.It is thus hypothesized that YP,L214 and G252 may contain new genes for resistance to bacterial blight.

Key words: Yuanjiang common wild rice, Infiltration line, Bacterial blight, Resistance gene

LU Yuanda, ZHONG Qiaofang, WANG Bo, ZHANG Dunyu, YIN Fuyou, WANG Lingxian, CHENG Zaiquan, CHEN Ling. Identification and Analysis of Resistance Genes to Bacterial Blight in Yuanjiang Common Wild Rice[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 199-205. doi: 10.7668/hbnxb.20193594.

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References 22

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菌株名称 Strain name	类型 Type
C1	中国1号代表菌株
C2	中国2号代表菌株
C3	中国3号代表菌株
C4	中国4号代表菌株
C5	中国5号代表菌株
C6	中国6号代表菌株
C7	中国7号代表菌株
C9	中国9号代表菌株
PXO99^A	菲律宾6号代表菌株

菌株名称 Strain name	类型 Type
C1	中国1号代表菌株
C2	中国2号代表菌株
C3	中国3号代表菌株
C4	中国4号代表菌株
C5	中国5号代表菌株
C6	中国6号代表菌株
C7	中国7号代表菌株
C9	中国9号代表菌株
PXO99^A	菲律宾6号代表菌株

编号 Code	引物序列(5'-3') Primer sequence (5'-3')	目的 Purpose
Xa7-kelong-F1	TCTACCGGGGTTCAGT	目标片段扩增
Xa7-kelong-R1	GACCCTTCGTATGGAATTGA
Xa10-kelong-F1	CACCAAAAGGTCCAACAC
Xa10-kelong-R1	GCCGGTTTCTCTTTATTAAC
Xa23-kelong-F1	AATTATGCGGCATCACTAAC
Xa23-kelong-R1	TAACAATCAAACCGAGGAGA
Actin-F	CAGGCCGTCCTCTCTCTGTA	实时荧光定量PCR
Actin-R	AAGGATAGCATGGGGGAGAG
OsPR1a-F	TTCATCACCTGCAACTACTCG
OsPR1a-R	TGCATAAACACGTAGCATAGCAT
OsPR10-F	CACCATCTACACCATGAAGC
OsPR10-R	AGCACATCCGACTTTAGGAC
qRT-PCR-Xa10-F	TCTTCTACCTCTCTGCCGCT
qRT-PCR-Xa10-R	CGGCAGCTATACGGGCATAA
qRT-PCR-Xa23-F	CTGTTTGGCTCCATCATGCC
qRT-PCR-Xa23-R	AAGATGAAGGCGTAGGCGAG

编号 Code	引物序列(5'-3') Primer sequence (5'-3')	目的 Purpose
Xa7-kelong-F1	TCTACCGGGGTTCAGT	目标片段扩增
Xa7-kelong-R1	GACCCTTCGTATGGAATTGA
Xa10-kelong-F1	CACCAAAAGGTCCAACAC
Xa10-kelong-R1	GCCGGTTTCTCTTTATTAAC
Xa23-kelong-F1	AATTATGCGGCATCACTAAC
Xa23-kelong-R1	TAACAATCAAACCGAGGAGA
Actin-F	CAGGCCGTCCTCTCTCTGTA	实时荧光定量PCR
Actin-R	AAGGATAGCATGGGGGAGAG
OsPR1a-F	TTCATCACCTGCAACTACTCG
OsPR1a-R	TGCATAAACACGTAGCATAGCAT
OsPR10-F	CACCATCTACACCATGAAGC
OsPR10-R	AGCACATCCGACTTTAGGAC
qRT-PCR-Xa10-F	TCTTCTACCTCTCTGCCGCT
qRT-PCR-Xa10-R	CGGCAGCTATACGGGCATAA
qRT-PCR-Xa23-F	CTGTTTGGCTCCATCATGCC
qRT-PCR-Xa23-R	AAGATGAAGGCGTAGGCGAG

基因 Gene	元江普通野生稻(♂) YP	合系35 (♀) HX35	L214 (IL) L214	G252 (IL) G252
Xa1	-	-	-	-
Xa3/Xa26	-	-	-	-
Xa4	-	-	-	-
xa5	-	-	-	-
Xa7	+-	-	-	+-
Xa10	++	-	-	-
xa13	-	-	-	-
Xa21	-	-	-	-
Xa23	-	++	-	+-
Xa27	-	-	-	-

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